Cg‐TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms 1
We have cloned and characterized a cDNA encoding Cg ‐TIMP, the first tissue inhibitor of metalloproteinase identified in mollusks. The isolated cDNA encodes a protein of 221 residues that has a domain organization similar to that of vertebrate TIMPs including a signal sequence, and the 12 cysteines...
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crwiley:10.1016/s0014-5793(01)02559-5 2024-06-02T08:05:36+00:00 Cg‐TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms 1 Montagnani, Caroline Le Roux, Frédérique Berthe, Franck Escoubas, Jean-Michel 2001 http://dx.doi.org/10.1016/s0014-5793(01)02559-5 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2801%2902559-5 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(01)02559-5 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2801%2902559-5 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor FEBS Letters volume 500, issue 1-2, page 64-70 ISSN 0014-5793 1873-3468 journal-article 2001 crwiley https://doi.org/10.1016/s0014-5793(01)02559-5 2024-05-03T11:31:42Z We have cloned and characterized a cDNA encoding Cg ‐TIMP, the first tissue inhibitor of metalloproteinase identified in mollusks. The isolated cDNA encodes a protein of 221 residues that has a domain organization similar to that of vertebrate TIMPs including a signal sequence, and the 12 cysteines characteristic of the TIMP signature. Analysis of Cg‐TIMP expression in adult oyster tissues, by Northern blot and in situ hybridization, indicates that Cg‐TIMP was only expressed in hemocytes which are the key components of defense mechanisms in mollusks. We also observed that Cg‐TIMP mRNA accumulated during shell damage and bacterial challenge. This pattern of expression suggests that Cg ‐TIMP may be an important factor in wound healing and defense mechanisms. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific FEBS Letters 500 1-2 64 70 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
We have cloned and characterized a cDNA encoding Cg ‐TIMP, the first tissue inhibitor of metalloproteinase identified in mollusks. The isolated cDNA encodes a protein of 221 residues that has a domain organization similar to that of vertebrate TIMPs including a signal sequence, and the 12 cysteines characteristic of the TIMP signature. Analysis of Cg‐TIMP expression in adult oyster tissues, by Northern blot and in situ hybridization, indicates that Cg‐TIMP was only expressed in hemocytes which are the key components of defense mechanisms in mollusks. We also observed that Cg‐TIMP mRNA accumulated during shell damage and bacterial challenge. This pattern of expression suggests that Cg ‐TIMP may be an important factor in wound healing and defense mechanisms. |
format |
Article in Journal/Newspaper |
author |
Montagnani, Caroline Le Roux, Frédérique Berthe, Franck Escoubas, Jean-Michel |
spellingShingle |
Montagnani, Caroline Le Roux, Frédérique Berthe, Franck Escoubas, Jean-Michel Cg‐TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms 1 |
author_facet |
Montagnani, Caroline Le Roux, Frédérique Berthe, Franck Escoubas, Jean-Michel |
author_sort |
Montagnani, Caroline |
title |
Cg‐TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms 1 |
title_short |
Cg‐TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms 1 |
title_full |
Cg‐TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms 1 |
title_fullStr |
Cg‐TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms 1 |
title_full_unstemmed |
Cg‐TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms 1 |
title_sort |
cg‐timp, an inducible tissue inhibitor of metalloproteinase from the pacific oyster crassostrea gigas with a potential role in wound healing and defense mechanisms 1 |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1016/s0014-5793(01)02559-5 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2801%2902559-5 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(01)02559-5 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2801%2902559-5 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
FEBS Letters volume 500, issue 1-2, page 64-70 ISSN 0014-5793 1873-3468 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/s0014-5793(01)02559-5 |
container_title |
FEBS Letters |
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500 |
container_issue |
1-2 |
container_start_page |
64 |
op_container_end_page |
70 |
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1800750469213061120 |