Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate
DNA methylation within promoter regions (PRDM) controls vertebrate early gene transcription and thereby development, but is neglected outside this group. However, epigenetic features in the oyster Crassostrea gigas suggest functional significance of PDRM in invertebrates. To investigate this, report...
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crwiley:10.1016/j.febslet.2015.04.043 2024-06-09T07:45:30+00:00 Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate Saint-Carlier, Emma Riviere, Guillaume Lower Normandy Regional Council 2015 http://dx.doi.org/10.1016/j.febslet.2015.04.043 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2015.04.043 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2015.04.043 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor FEBS Letters volume 589, issue 13, page 1459-1466 ISSN 0014-5793 1873-3468 journal-article 2015 crwiley https://doi.org/10.1016/j.febslet.2015.04.043 2024-05-16T14:24:40Z DNA methylation within promoter regions (PRDM) controls vertebrate early gene transcription and thereby development, but is neglected outside this group. However, epigenetic features in the oyster Crassostrea gigas suggest functional significance of PDRM in invertebrates. To investigate this, reporter constructs containing in vitro methylated oyster Hox gene promoters were transfected into oyster embryos. The influence of in vivo methylation was studied using bisulfite sequencing and DNA methyltransferase inhibition during development. Our results demonstrate that methylation controls the transcriptional activity of the promoters investigated, unraveling a functional role for PRDM in a lophotrochozoan, an important finding regarding the evolution of epigenetic regulation. Article in Journal/Newspaper Crassostrea gigas Wiley Online Library FEBS Letters 589 13 1459 1466 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
DNA methylation within promoter regions (PRDM) controls vertebrate early gene transcription and thereby development, but is neglected outside this group. However, epigenetic features in the oyster Crassostrea gigas suggest functional significance of PDRM in invertebrates. To investigate this, reporter constructs containing in vitro methylated oyster Hox gene promoters were transfected into oyster embryos. The influence of in vivo methylation was studied using bisulfite sequencing and DNA methyltransferase inhibition during development. Our results demonstrate that methylation controls the transcriptional activity of the promoters investigated, unraveling a functional role for PRDM in a lophotrochozoan, an important finding regarding the evolution of epigenetic regulation. |
author2 |
Lower Normandy Regional Council |
format |
Article in Journal/Newspaper |
author |
Saint-Carlier, Emma Riviere, Guillaume |
spellingShingle |
Saint-Carlier, Emma Riviere, Guillaume Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate |
author_facet |
Saint-Carlier, Emma Riviere, Guillaume |
author_sort |
Saint-Carlier, Emma |
title |
Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate |
title_short |
Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate |
title_full |
Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate |
title_fullStr |
Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate |
title_full_unstemmed |
Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate |
title_sort |
regulation of hox orthologues in the oyster crassostrea gigas evidences a functional role for promoter dna methylation in an invertebrate |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1016/j.febslet.2015.04.043 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2015.04.043 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2015.04.043 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
FEBS Letters volume 589, issue 13, page 1459-1466 ISSN 0014-5793 1873-3468 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/j.febslet.2015.04.043 |
container_title |
FEBS Letters |
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589 |
container_issue |
13 |
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1459 |
op_container_end_page |
1466 |
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1801374877727326208 |