Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean
Proteorhodopsin (PR), a retinal‐containing seven transmembrane helix protein, functions as a light‐driven proton pump. Using PCR, we isolated 18 PR variants originating from the surface of the Arctic Ocean. Their absorption maxima were between 517 and 546 nm at pH 7. One of the isolates turned out t...
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crwiley:10.1016/j.febslet.2008.04.025 2024-09-15T17:53:13+00:00 Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean Jung, Jae Yong Choi, Ah Reum Lee, Yoo Kyung Lee, Hong Kum Jung, Kwang-Hwan 2008 http://dx.doi.org/10.1016/j.febslet.2008.04.025 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2008.04.025 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2008.04.025 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor FEBS Letters volume 582, issue 12, page 1679-1684 ISSN 0014-5793 1873-3468 journal-article 2008 crwiley https://doi.org/10.1016/j.febslet.2008.04.025 2024-08-30T04:11:39Z Proteorhodopsin (PR), a retinal‐containing seven transmembrane helix protein, functions as a light‐driven proton pump. Using PCR, we isolated 18 PR variants originating from the surface of the Arctic Ocean. Their absorption maxima were between 517 and 546 nm at pH 7. One of the isolates turned out to be identical to GPR (green light‐absorbing proteorhodopsin) from Monterey Bay. Interestingly, 10 isolates had replaced a tyrosine in the retinal‐binding site (Tyr200 in GPR) with Asn. They showed a slower photocycle, more blue‐shifted absorption maxima at pH 10, and relatively larger Δ H and Δ S of activation of the transition between the O intermediate and the ground state compared to GPR. Article in Journal/Newspaper Arctic Ocean Wiley Online Library FEBS Letters 582 12 1679 1684 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Proteorhodopsin (PR), a retinal‐containing seven transmembrane helix protein, functions as a light‐driven proton pump. Using PCR, we isolated 18 PR variants originating from the surface of the Arctic Ocean. Their absorption maxima were between 517 and 546 nm at pH 7. One of the isolates turned out to be identical to GPR (green light‐absorbing proteorhodopsin) from Monterey Bay. Interestingly, 10 isolates had replaced a tyrosine in the retinal‐binding site (Tyr200 in GPR) with Asn. They showed a slower photocycle, more blue‐shifted absorption maxima at pH 10, and relatively larger Δ H and Δ S of activation of the transition between the O intermediate and the ground state compared to GPR. |
format |
Article in Journal/Newspaper |
author |
Jung, Jae Yong Choi, Ah Reum Lee, Yoo Kyung Lee, Hong Kum Jung, Kwang-Hwan |
spellingShingle |
Jung, Jae Yong Choi, Ah Reum Lee, Yoo Kyung Lee, Hong Kum Jung, Kwang-Hwan Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean |
author_facet |
Jung, Jae Yong Choi, Ah Reum Lee, Yoo Kyung Lee, Hong Kum Jung, Kwang-Hwan |
author_sort |
Jung, Jae Yong |
title |
Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean |
title_short |
Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean |
title_full |
Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean |
title_fullStr |
Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean |
title_full_unstemmed |
Spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the Arctic Ocean |
title_sort |
spectroscopic and photochemical analysis of proteorhodopsin variants from the surface of the arctic ocean |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1016/j.febslet.2008.04.025 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2008.04.025 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2008.04.025 |
genre |
Arctic Ocean |
genre_facet |
Arctic Ocean |
op_source |
FEBS Letters volume 582, issue 12, page 1679-1684 ISSN 0014-5793 1873-3468 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/j.febslet.2008.04.025 |
container_title |
FEBS Letters |
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582 |
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12 |
container_start_page |
1679 |
op_container_end_page |
1684 |
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1810295228608806912 |