Formate binding to ferric wild type and mutant myoglobins thermodynamic and X‐ray crystallographic study
The X‐ray crystal structure of the formate derivative of ferric loggerhead sea turtle ( Caretta caretta ) Mb has been determined at 2.0 Å resolution ( R = 0.164) by difference Fourier techniques. Formate, sitting in the central part of the heme distal site, is coordinated to the heme iron as unident...
Published in: | FEBS Letters |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1995
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1016/0014-5793(94)01324-t https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2894%2901324-T https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(94)01324-T https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2894%2901324-T |
id |
crwiley:10.1016/0014-5793(94)01324-t |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1016/0014-5793(94)01324-t 2024-06-02T08:14:52+00:00 Formate binding to ferric wild type and mutant myoglobins thermodynamic and X‐ray crystallographic study Leci, Edlira Brancaccio, Andrea Cutruzzolà, Francesca Allocatelli, Carlo Travaglini Tarricone, Cataldo Bolognesi, Martino Desideri, Alessandro Ascenzi, Paolo 1995 http://dx.doi.org/10.1016/0014-5793(94)01324-t https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2894%2901324-T https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(94)01324-T https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2894%2901324-T en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor FEBS Letters volume 357, issue 3, page 227-229 ISSN 0014-5793 1873-3468 journal-article 1995 crwiley https://doi.org/10.1016/0014-5793(94)01324-t 2024-05-03T11:31:08Z The X‐ray crystal structure of the formate derivative of ferric loggerhead sea turtle ( Caretta caretta ) Mb has been determined at 2.0 Å resolution ( R = 0.164) by difference Fourier techniques. Formate, sitting in the central part of the heme distal site, is coordinated to the heme iron as unidentate ligand, through the O1 oxygen atom, and is hydrogen bonded to the distal His 64 (E7) NE2 atom through O2. Thermodynamics for formate binding to ferric loggerhead sea turtle Mb, sperm whale Mb, Aplysia limacina Mb, as well as to the VR and VRS mutants of sperm whale Mb were obtained between pH 4.5 and 8.5, at 20.0°C. These results, representing the first structure of a ferric hemoprotein:formate complex solved by X‐ray crystallography, outline the role of amino acid residues at positions E7, F8 and E10 in modulating ligand binding properties of oxygen carrying proteins. Article in Journal/Newspaper Sperm whale Wiley Online Library FEBS Letters 357 3 227 229 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
The X‐ray crystal structure of the formate derivative of ferric loggerhead sea turtle ( Caretta caretta ) Mb has been determined at 2.0 Å resolution ( R = 0.164) by difference Fourier techniques. Formate, sitting in the central part of the heme distal site, is coordinated to the heme iron as unidentate ligand, through the O1 oxygen atom, and is hydrogen bonded to the distal His 64 (E7) NE2 atom through O2. Thermodynamics for formate binding to ferric loggerhead sea turtle Mb, sperm whale Mb, Aplysia limacina Mb, as well as to the VR and VRS mutants of sperm whale Mb were obtained between pH 4.5 and 8.5, at 20.0°C. These results, representing the first structure of a ferric hemoprotein:formate complex solved by X‐ray crystallography, outline the role of amino acid residues at positions E7, F8 and E10 in modulating ligand binding properties of oxygen carrying proteins. |
format |
Article in Journal/Newspaper |
author |
Leci, Edlira Brancaccio, Andrea Cutruzzolà, Francesca Allocatelli, Carlo Travaglini Tarricone, Cataldo Bolognesi, Martino Desideri, Alessandro Ascenzi, Paolo |
spellingShingle |
Leci, Edlira Brancaccio, Andrea Cutruzzolà, Francesca Allocatelli, Carlo Travaglini Tarricone, Cataldo Bolognesi, Martino Desideri, Alessandro Ascenzi, Paolo Formate binding to ferric wild type and mutant myoglobins thermodynamic and X‐ray crystallographic study |
author_facet |
Leci, Edlira Brancaccio, Andrea Cutruzzolà, Francesca Allocatelli, Carlo Travaglini Tarricone, Cataldo Bolognesi, Martino Desideri, Alessandro Ascenzi, Paolo |
author_sort |
Leci, Edlira |
title |
Formate binding to ferric wild type and mutant myoglobins thermodynamic and X‐ray crystallographic study |
title_short |
Formate binding to ferric wild type and mutant myoglobins thermodynamic and X‐ray crystallographic study |
title_full |
Formate binding to ferric wild type and mutant myoglobins thermodynamic and X‐ray crystallographic study |
title_fullStr |
Formate binding to ferric wild type and mutant myoglobins thermodynamic and X‐ray crystallographic study |
title_full_unstemmed |
Formate binding to ferric wild type and mutant myoglobins thermodynamic and X‐ray crystallographic study |
title_sort |
formate binding to ferric wild type and mutant myoglobins thermodynamic and x‐ray crystallographic study |
publisher |
Wiley |
publishDate |
1995 |
url |
http://dx.doi.org/10.1016/0014-5793(94)01324-t https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2894%2901324-T https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(94)01324-T https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2894%2901324-T |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
FEBS Letters volume 357, issue 3, page 227-229 ISSN 0014-5793 1873-3468 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/0014-5793(94)01324-t |
container_title |
FEBS Letters |
container_volume |
357 |
container_issue |
3 |
container_start_page |
227 |
op_container_end_page |
229 |
_version_ |
1800738865520050176 |