Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10
The contribution to oxygen stabilization of a tyrosine residue in topological position (B10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (B10), (E7) and (E10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that...
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1994
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crwiley:10.1016/0014-5793(94)00918-x 2024-06-02T08:14:51+00:00 Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10 Allocatelli, Carlo Travaglini Cutruzzolà, Francesca Brancaccio, Andrea Vallone, Beatrice Brunori, Maurizio 1994 http://dx.doi.org/10.1016/0014-5793(94)00918-x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2894%2900918-X https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(94)00918-X https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2894%2900918-X en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor FEBS Letters volume 352, issue 1, page 63-66 ISSN 0014-5793 1873-3468 journal-article 1994 crwiley https://doi.org/10.1016/0014-5793(94)00918-x 2024-05-03T11:08:44Z The contribution to oxygen stabilization of a tyrosine residue in topological position (B10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (B10), (E7) and (E10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that of the nematode Ascaris suum . Kinetic and equilibrium experiments with the gaseous ligands oxygen and carbon monoxide show that indeed the introduction of tyrosine (B10), together with replacement of the distal histidine (E7) with glutamine, is associated with a large decrease in the oxygen dissociation rate constant. Our results are consistent with the possible formation in the distal pocket of two hydrogen bonds with the iron‐bound oxygen. Article in Journal/Newspaper Sperm whale Wiley Online Library FEBS Letters 352 1 63 66 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
The contribution to oxygen stabilization of a tyrosine residue in topological position (B10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (B10), (E7) and (E10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that of the nematode Ascaris suum . Kinetic and equilibrium experiments with the gaseous ligands oxygen and carbon monoxide show that indeed the introduction of tyrosine (B10), together with replacement of the distal histidine (E7) with glutamine, is associated with a large decrease in the oxygen dissociation rate constant. Our results are consistent with the possible formation in the distal pocket of two hydrogen bonds with the iron‐bound oxygen. |
format |
Article in Journal/Newspaper |
author |
Allocatelli, Carlo Travaglini Cutruzzolà, Francesca Brancaccio, Andrea Vallone, Beatrice Brunori, Maurizio |
spellingShingle |
Allocatelli, Carlo Travaglini Cutruzzolà, Francesca Brancaccio, Andrea Vallone, Beatrice Brunori, Maurizio Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10 |
author_facet |
Allocatelli, Carlo Travaglini Cutruzzolà, Francesca Brancaccio, Andrea Vallone, Beatrice Brunori, Maurizio |
author_sort |
Allocatelli, Carlo Travaglini |
title |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10 |
title_short |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10 |
title_full |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10 |
title_fullStr |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10 |
title_full_unstemmed |
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10 |
title_sort |
engineering ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine b10 |
publisher |
Wiley |
publishDate |
1994 |
url |
http://dx.doi.org/10.1016/0014-5793(94)00918-x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2894%2900918-X https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(94)00918-X https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2894%2900918-X |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
FEBS Letters volume 352, issue 1, page 63-66 ISSN 0014-5793 1873-3468 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/0014-5793(94)00918-x |
container_title |
FEBS Letters |
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352 |
container_issue |
1 |
container_start_page |
63 |
op_container_end_page |
66 |
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1800738859002101760 |