Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 Å resolution
The three‐dimensional structure of sperm whale myoglobin His 64 (E7)→Val,Thr 64 (E10)→Arg double mutant has been studied by X‐ray crystallography at 1.6 Å resolution, and refined to a crystallographic R ‐factor of 0.197. The Arg 67 (E10) side chain is extended in the direction of the ligand binding...
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crwiley:10.1016/0014-5793(93)81647-i 2024-06-02T08:14:52+00:00 Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 Å resolution Rizzi, Menico Bolognesi, Martino Coda, Alessandro Cutruzzolà, Francesca Allocatelli, Carlo Travaglini Brancaccio, Andrea Brunori, Maurizio 1993 http://dx.doi.org/10.1016/0014-5793(93)81647-i https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2893%2981647-I https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(93)81647-I https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2893%2981647-I en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor FEBS Letters volume 320, issue 1, page 13-16 ISSN 0014-5793 1873-3468 journal-article 1993 crwiley https://doi.org/10.1016/0014-5793(93)81647-i 2024-05-03T11:23:39Z The three‐dimensional structure of sperm whale myoglobin His 64 (E7)→Val,Thr 64 (E10)→Arg double mutant has been studied by X‐ray crystallography at 1.6 Å resolution, and refined to a crystallographic R ‐factor of 0.197. The Arg 67 (E10) side chain is extended in the direction of the ligand binding site, and its NH1 atom is at a distance of 3.11 Å from the NH1 atom of Arg 45 (CD3), which is also pointing towards the distal site. Both are kept in this position by hydrogen bonding and electrostatic interactions with a solvent sulfate ion, located amongst the two, on the protein surface. No liganded water molecule is present at the sixth coordination position of the Fe(III) heme. Article in Journal/Newspaper Sperm whale Wiley Online Library FEBS Letters 320 1 13 16 |
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Wiley Online Library |
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crwiley |
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English |
description |
The three‐dimensional structure of sperm whale myoglobin His 64 (E7)→Val,Thr 64 (E10)→Arg double mutant has been studied by X‐ray crystallography at 1.6 Å resolution, and refined to a crystallographic R ‐factor of 0.197. The Arg 67 (E10) side chain is extended in the direction of the ligand binding site, and its NH1 atom is at a distance of 3.11 Å from the NH1 atom of Arg 45 (CD3), which is also pointing towards the distal site. Both are kept in this position by hydrogen bonding and electrostatic interactions with a solvent sulfate ion, located amongst the two, on the protein surface. No liganded water molecule is present at the sixth coordination position of the Fe(III) heme. |
format |
Article in Journal/Newspaper |
author |
Rizzi, Menico Bolognesi, Martino Coda, Alessandro Cutruzzolà, Francesca Allocatelli, Carlo Travaglini Brancaccio, Andrea Brunori, Maurizio |
spellingShingle |
Rizzi, Menico Bolognesi, Martino Coda, Alessandro Cutruzzolà, Francesca Allocatelli, Carlo Travaglini Brancaccio, Andrea Brunori, Maurizio Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 Å resolution |
author_facet |
Rizzi, Menico Bolognesi, Martino Coda, Alessandro Cutruzzolà, Francesca Allocatelli, Carlo Travaglini Brancaccio, Andrea Brunori, Maurizio |
author_sort |
Rizzi, Menico |
title |
Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 Å resolution |
title_short |
Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 Å resolution |
title_full |
Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 Å resolution |
title_fullStr |
Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 Å resolution |
title_full_unstemmed |
Crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 Å resolution |
title_sort |
crystal structure of a distal site double mutant of sperm whale myoglobin at 1.6 å resolution |
publisher |
Wiley |
publishDate |
1993 |
url |
http://dx.doi.org/10.1016/0014-5793(93)81647-i https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2893%2981647-I https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(93)81647-I https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2893%2981647-I |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
FEBS Letters volume 320, issue 1, page 13-16 ISSN 0014-5793 1873-3468 |
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http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/0014-5793(93)81647-i |
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FEBS Letters |
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320 |
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13 |
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16 |
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1800738861153779712 |