Are microtubules cold‐stable in the Atlantic cod, Gadus morhua?

• Published in: FEBS Letters
• Main Authors: Strömberg, E., Jönsson, A.-C., Wallin, M.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1986
• Subjects: atlantic cod; Gadus morhua
• Online Access: http://dx.doi.org/10.1016/0014-5793(86)81396-5; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2886%2981396-5; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(86)81396-5; https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2886%2981396-5

The amount of axonally transported proteins in the nervus splanchnicus of cod ( Gadus morhua ) was found to be temperature‐dependent in vitro, with an optimum at 8°C. The transport was markedly reduced at 2°C, probably caused by decreased protein synthesis rather than disassembly of microtubules. Microtubules were isolated from cod brain by cycles of assembly‐disassembly. These microtubules were cold‐labile, had a low amount of microtubule‐associated proteins and a high critical concentration for assembly. The possibilities exist that a cold‐stabilizing factor or cold‐stable microtubules are lost during the preparation, or that cold‐stable microtubules are components of the peripheral axons only.