Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes
Hepatocytes of Salmo salar in primary culture form confluent monolayers and can be maintained at 11 °C in serum‐free medium for 8 days with minimal cell loss. Cultured hepatocytes from immature male salmon contain estrogen receptor both in nuclear and cytosol fractions (2000 and 2400 sites/cell, res...
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1986
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Online Access: | http://dx.doi.org/10.1016/0014-5793(86)80174-0 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2886%2980174-0 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(86)80174-0 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2886%2980174-0 |
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crwiley:10.1016/0014-5793(86)80174-0 2024-06-02T08:13:59+00:00 Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes Mommsen, Thomas P. Lazier, Catherine B. 1986 http://dx.doi.org/10.1016/0014-5793(86)80174-0 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2886%2980174-0 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(86)80174-0 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2886%2980174-0 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor FEBS Letters volume 195, issue 1-2, page 269-271 ISSN 0014-5793 1873-3468 journal-article 1986 crwiley https://doi.org/10.1016/0014-5793(86)80174-0 2024-05-03T11:51:50Z Hepatocytes of Salmo salar in primary culture form confluent monolayers and can be maintained at 11 °C in serum‐free medium for 8 days with minimal cell loss. Cultured hepatocytes from immature male salmon contain estrogen receptor both in nuclear and cytosol fractions (2000 and 2400 sites/cell, respectively). A single addition of estradiol results in an increase in the nuclear receptor to a level of 23 000 sites/cell after 24 h. This nuclear receptor concentration is similar to that in liver of estrogen‐treated salmon in vivo, and is much higher than has been found for any other egg‐laying vertebrate. The cultured salmon hepatocytes thus represent a highly sensitive system for the study of estrogen receptor dynamics and vitellogenesis in vitro. Article in Journal/Newspaper Salmo salar Wiley Online Library FEBS Letters 195 1-2 269 271 |
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Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Hepatocytes of Salmo salar in primary culture form confluent monolayers and can be maintained at 11 °C in serum‐free medium for 8 days with minimal cell loss. Cultured hepatocytes from immature male salmon contain estrogen receptor both in nuclear and cytosol fractions (2000 and 2400 sites/cell, respectively). A single addition of estradiol results in an increase in the nuclear receptor to a level of 23 000 sites/cell after 24 h. This nuclear receptor concentration is similar to that in liver of estrogen‐treated salmon in vivo, and is much higher than has been found for any other egg‐laying vertebrate. The cultured salmon hepatocytes thus represent a highly sensitive system for the study of estrogen receptor dynamics and vitellogenesis in vitro. |
format |
Article in Journal/Newspaper |
author |
Mommsen, Thomas P. Lazier, Catherine B. |
spellingShingle |
Mommsen, Thomas P. Lazier, Catherine B. Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes |
author_facet |
Mommsen, Thomas P. Lazier, Catherine B. |
author_sort |
Mommsen, Thomas P. |
title |
Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes |
title_short |
Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes |
title_full |
Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes |
title_fullStr |
Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes |
title_full_unstemmed |
Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes |
title_sort |
stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes |
publisher |
Wiley |
publishDate |
1986 |
url |
http://dx.doi.org/10.1016/0014-5793(86)80174-0 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2886%2980174-0 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(86)80174-0 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2886%2980174-0 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
FEBS Letters volume 195, issue 1-2, page 269-271 ISSN 0014-5793 1873-3468 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1016/0014-5793(86)80174-0 |
container_title |
FEBS Letters |
container_volume |
195 |
container_issue |
1-2 |
container_start_page |
269 |
op_container_end_page |
271 |
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1800737672167161856 |