Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids
Abstract Biodiesel is conventionally produced by alkaline‐catalyzed transesterification, which requires high‐purity oils. However, low‐quality oils can be used as feedstocks for the production of biodiesel by enzyme‐catalyzed reactions. The use of enzymes has several advantages, such as the absence...
Published in: | Journal of the American Oil Chemists' Society |
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Online Access: | http://dx.doi.org/10.1007/s11746-016-2901-y http://link.springer.com/content/pdf/10.1007/s11746-016-2901-y.pdf http://link.springer.com/article/10.1007/s11746-016-2901-y/fulltext.html |
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crwiley:10.1007/s11746-016-2901-y 2024-09-09T19:10:16+00:00 Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids Vescovi, Vinicius Rojas, Mayerlenis Jimenez Baraldo, Anderson Botta, Daniel Carrero Santana, Felipe Augusto Montes Costa, Julia Piva Machado, Mariana Stefani Honda, Vitor Kasuyoshi de Lima Camargo Giordano, Raquel Tardioli, Paulo Waldir São Paulo Research Foundation (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 2016 http://dx.doi.org/10.1007/s11746-016-2901-y http://link.springer.com/content/pdf/10.1007/s11746-016-2901-y.pdf http://link.springer.com/article/10.1007/s11746-016-2901-y/fulltext.html en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://www.springer.com/tdm Journal of the American Oil Chemists' Society volume 93, issue 12, page 1615-1624 ISSN 0003-021X 1558-9331 journal-article 2016 crwiley https://doi.org/10.1007/s11746-016-2901-y 2024-08-06T04:13:57Z Abstract Biodiesel is conventionally produced by alkaline‐catalyzed transesterification, which requires high‐purity oils. However, low‐quality oils can be used as feedstocks for the production of biodiesel by enzyme‐catalyzed reactions. The use of enzymes has several advantages, such as the absence of saponification side reactions, production of high‐purity glycerol co‐product, and low‐cost downstream processing. In this work, biodiesel was produced from lipase‐catalyzed hydrolysis of waste cooking oil (WCO) followed by esterification of the hydrolyzed WCO (HWCO). The hydrolysis of acylglycerols was carried out at 30 °C in salt‐free water (WCO/water ratio of 1:4, v/v) and the esterification of HWCO was carried out at 40 °C with ethanol in a solvent‐free medium (HWCO/ethanol molar ratio of 1:7). The hydrolysis and esterification steps were carried out using immobilized Thermomyces lanuginosus lipase (TLL/WCO ratio of 1:5.6, w/w) and immobilized Candida antarctica lipase B (10 wt%, CALB/HWCO) as biocatalysts, respectively. The hydrolysis of acylglycerols was almost complete after 12 h (ca. 94 %), and in the esterification step, the conversion was around 90 % after 6 h. The purified biodiesel had 91.8 wt% of fatty acid ethyl esters, 0.53 wt% of acylglycerols, 0.003 wt% of free glycerol, viscosity of 4.59 cP, and acid value of 10.88 mg KOH/g. Reuse hydrolysis and esterification assays showed that the immobilized enzymes could be recycled five times in 10‐h batches, under the conditions described above. TLL was greatly inactivated under the assay conditions, whereas CALB remained fully active. The results showed that WCO is a promising feedstock for use in the production of biodiesel. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 93 12 1615 1624 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Biodiesel is conventionally produced by alkaline‐catalyzed transesterification, which requires high‐purity oils. However, low‐quality oils can be used as feedstocks for the production of biodiesel by enzyme‐catalyzed reactions. The use of enzymes has several advantages, such as the absence of saponification side reactions, production of high‐purity glycerol co‐product, and low‐cost downstream processing. In this work, biodiesel was produced from lipase‐catalyzed hydrolysis of waste cooking oil (WCO) followed by esterification of the hydrolyzed WCO (HWCO). The hydrolysis of acylglycerols was carried out at 30 °C in salt‐free water (WCO/water ratio of 1:4, v/v) and the esterification of HWCO was carried out at 40 °C with ethanol in a solvent‐free medium (HWCO/ethanol molar ratio of 1:7). The hydrolysis and esterification steps were carried out using immobilized Thermomyces lanuginosus lipase (TLL/WCO ratio of 1:5.6, w/w) and immobilized Candida antarctica lipase B (10 wt%, CALB/HWCO) as biocatalysts, respectively. The hydrolysis of acylglycerols was almost complete after 12 h (ca. 94 %), and in the esterification step, the conversion was around 90 % after 6 h. The purified biodiesel had 91.8 wt% of fatty acid ethyl esters, 0.53 wt% of acylglycerols, 0.003 wt% of free glycerol, viscosity of 4.59 cP, and acid value of 10.88 mg KOH/g. Reuse hydrolysis and esterification assays showed that the immobilized enzymes could be recycled five times in 10‐h batches, under the conditions described above. TLL was greatly inactivated under the assay conditions, whereas CALB remained fully active. The results showed that WCO is a promising feedstock for use in the production of biodiesel. |
author2 |
São Paulo Research Foundation (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
format |
Article in Journal/Newspaper |
author |
Vescovi, Vinicius Rojas, Mayerlenis Jimenez Baraldo, Anderson Botta, Daniel Carrero Santana, Felipe Augusto Montes Costa, Julia Piva Machado, Mariana Stefani Honda, Vitor Kasuyoshi de Lima Camargo Giordano, Raquel Tardioli, Paulo Waldir |
spellingShingle |
Vescovi, Vinicius Rojas, Mayerlenis Jimenez Baraldo, Anderson Botta, Daniel Carrero Santana, Felipe Augusto Montes Costa, Julia Piva Machado, Mariana Stefani Honda, Vitor Kasuyoshi de Lima Camargo Giordano, Raquel Tardioli, Paulo Waldir Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids |
author_facet |
Vescovi, Vinicius Rojas, Mayerlenis Jimenez Baraldo, Anderson Botta, Daniel Carrero Santana, Felipe Augusto Montes Costa, Julia Piva Machado, Mariana Stefani Honda, Vitor Kasuyoshi de Lima Camargo Giordano, Raquel Tardioli, Paulo Waldir |
author_sort |
Vescovi, Vinicius |
title |
Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids |
title_short |
Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids |
title_full |
Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids |
title_fullStr |
Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids |
title_full_unstemmed |
Lipase‐Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids |
title_sort |
lipase‐catalyzed production of biodiesel by hydrolysis of waste cooking oil followed by esterification of free fatty acids |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1007/s11746-016-2901-y http://link.springer.com/content/pdf/10.1007/s11746-016-2901-y.pdf http://link.springer.com/article/10.1007/s11746-016-2901-y/fulltext.html |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 93, issue 12, page 1615-1624 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://www.springer.com/tdm |
op_doi |
https://doi.org/10.1007/s11746-016-2901-y |
container_title |
Journal of the American Oil Chemists' Society |
container_volume |
93 |
container_issue |
12 |
container_start_page |
1615 |
op_container_end_page |
1624 |
_version_ |
1809825016114577408 |