Activity of immobilized Thermomyces lanuginosus and Candida antarctica B Lipases in Interesterification Reactions: Effect of the Aqueous Microenvironment
Abstract Lipases are enzymes used in numerous reactions of industrial interest. Depending on their aqueous microenvironment, lipases can catalyze hydrolysis or, conversely, organic synthesis like interesterification. This reaction can be used as a method to modify the physical and chemical propertie...
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crwiley:10.1007/s11746-013-2256-6 2024-06-02T07:57:04+00:00 Activity of immobilized Thermomyces lanuginosus and Candida antarctica B Lipases in Interesterification Reactions: Effect of the Aqueous Microenvironment Pérignon, Marlène Lecomte, Jérôme Pina, Michel Renault, Anne Simonneau‐Deve, Camille Villeneuve, Pierre 2013 http://dx.doi.org/10.1007/s11746-013-2256-6 http://link.springer.com/content/pdf/10.1007/s11746-013-2256-6 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 90, issue 8, page 1151-1156 ISSN 0003-021X 1558-9331 journal-article 2013 crwiley https://doi.org/10.1007/s11746-013-2256-6 2024-05-03T10:43:49Z Abstract Lipases are enzymes used in numerous reactions of industrial interest. Depending on their aqueous microenvironment, lipases can catalyze hydrolysis or, conversely, organic synthesis like interesterification. This reaction can be used as a method to modify the physical and chemical properties of fats and oils, a basic process for production of “structured lipids”. For such synthesis reactions, thermodynamic water activity (a w ) of the catalyst is generally the most important parameter to control. Actually, it will directly determine the performance of the synthesis, namely its yield, selectivity and stability. Effect of the a w on the activity of immobilized Thermomyces lanuginosus and Candida antarctica B lipases in interesterification reactions was studied. Water sorption and desorption isotherms were determined, showing a phenomenon of hysteresis for the Thermomyces lanuginosus lipase. Evaluation of the influence of a w on reaction yields revealed that the IE activity tends to increase with the water activity of immobilized Thermomyces lanuginosus lipase. In contrast, a w had little influence in the case of the Candida antarctica B lipase. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 90 8 1151 1156 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Lipases are enzymes used in numerous reactions of industrial interest. Depending on their aqueous microenvironment, lipases can catalyze hydrolysis or, conversely, organic synthesis like interesterification. This reaction can be used as a method to modify the physical and chemical properties of fats and oils, a basic process for production of “structured lipids”. For such synthesis reactions, thermodynamic water activity (a w ) of the catalyst is generally the most important parameter to control. Actually, it will directly determine the performance of the synthesis, namely its yield, selectivity and stability. Effect of the a w on the activity of immobilized Thermomyces lanuginosus and Candida antarctica B lipases in interesterification reactions was studied. Water sorption and desorption isotherms were determined, showing a phenomenon of hysteresis for the Thermomyces lanuginosus lipase. Evaluation of the influence of a w on reaction yields revealed that the IE activity tends to increase with the water activity of immobilized Thermomyces lanuginosus lipase. In contrast, a w had little influence in the case of the Candida antarctica B lipase. |
format |
Article in Journal/Newspaper |
author |
Pérignon, Marlène Lecomte, Jérôme Pina, Michel Renault, Anne Simonneau‐Deve, Camille Villeneuve, Pierre |
spellingShingle |
Pérignon, Marlène Lecomte, Jérôme Pina, Michel Renault, Anne Simonneau‐Deve, Camille Villeneuve, Pierre Activity of immobilized Thermomyces lanuginosus and Candida antarctica B Lipases in Interesterification Reactions: Effect of the Aqueous Microenvironment |
author_facet |
Pérignon, Marlène Lecomte, Jérôme Pina, Michel Renault, Anne Simonneau‐Deve, Camille Villeneuve, Pierre |
author_sort |
Pérignon, Marlène |
title |
Activity of immobilized Thermomyces lanuginosus and Candida antarctica B Lipases in Interesterification Reactions: Effect of the Aqueous Microenvironment |
title_short |
Activity of immobilized Thermomyces lanuginosus and Candida antarctica B Lipases in Interesterification Reactions: Effect of the Aqueous Microenvironment |
title_full |
Activity of immobilized Thermomyces lanuginosus and Candida antarctica B Lipases in Interesterification Reactions: Effect of the Aqueous Microenvironment |
title_fullStr |
Activity of immobilized Thermomyces lanuginosus and Candida antarctica B Lipases in Interesterification Reactions: Effect of the Aqueous Microenvironment |
title_full_unstemmed |
Activity of immobilized Thermomyces lanuginosus and Candida antarctica B Lipases in Interesterification Reactions: Effect of the Aqueous Microenvironment |
title_sort |
activity of immobilized thermomyces lanuginosus and candida antarctica b lipases in interesterification reactions: effect of the aqueous microenvironment |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1007/s11746-013-2256-6 http://link.springer.com/content/pdf/10.1007/s11746-013-2256-6 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 90, issue 8, page 1151-1156 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11746-013-2256-6 |
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Journal of the American Oil Chemists' Society |
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90 |
container_issue |
8 |
container_start_page |
1151 |
op_container_end_page |
1156 |
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1800738484177076224 |