Lipase‐Catalyzed Synthesis of Medium‐Long‐Medium Type Structured Lipids Using Tricaprylin and Trilinolenin as Substrate Models
Abstract The synthesis of medium‐long‐medium type structured lipids (SL) by the interesterification of tricaprylin (TC) and trilinolenin (TLN), using selected commercial lipases from Rhizomucor miehei (Lipozyme RM IM) and Candida antarctica (Novozym 435) was investigated. Although the bioconversion...
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crwiley:10.1007/s11746-012-2185-9 2024-06-02T07:57:10+00:00 Lipase‐Catalyzed Synthesis of Medium‐Long‐Medium Type Structured Lipids Using Tricaprylin and Trilinolenin as Substrate Models Bai, Shan Aziz, Sarya Khodadadi, Maryam Bou Mitri, Christelle St‐Louis, Richard Kermasha, Selim Natural Sciences and Engineering Research Council of Canada 2012 http://dx.doi.org/10.1007/s11746-012-2185-9 https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-012-2185-9 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 90, issue 3, page 377-389 ISSN 0003-021X 1558-9331 journal-article 2012 crwiley https://doi.org/10.1007/s11746-012-2185-9 2024-05-03T10:45:19Z Abstract The synthesis of medium‐long‐medium type structured lipids (SL) by the interesterification of tricaprylin (TC) and trilinolenin (TLN), using selected commercial lipases from Rhizomucor miehei (Lipozyme RM IM) and Candida antarctica (Novozym 435) was investigated. Although the bioconversion yield (BY) for Lipozyme RM IM (24.7 %) was close to that for Novozym 435 (24.0 %), the initial enzyme activity was 6.3 μmol CLnC/g enzyme/min and 1.6 μmol CLnC/g enzyme/min, respectively. Lipozyme RM IM was subsequently selected for further investigation. The structural analyses of SL indicated that the major products were 1,3‐dicapryl‐2‐linolenyl glycerol (CLnC) and 1(3)‐capryl‐2,3(1)‐dilinolenyl glycerol (CLnLn). In order to optimize the BY, selected parameters were investigated. The experimental results showed that using hexane as the reaction medium, at an initial water activity ( a w ) of 0.06, 10 mg solid enzyme/mL, substrate molar ratio of TC to TLN of 6:1 and a reaction time of 9 h, resulted in the highest BY (73.2 %). Using the optimized conditions, the effects of TLN concentration and other selective parameters, including the denaturation of the enzyme, controlling the a w and the addition of silica gel, on the mass productivity ( P M ), enzymatic productivity ( P E ) and volumetric productivity ( P V ) of the interesterification reaction, were also investigated. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 90 3 377 389 |
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Wiley Online Library |
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English |
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Abstract The synthesis of medium‐long‐medium type structured lipids (SL) by the interesterification of tricaprylin (TC) and trilinolenin (TLN), using selected commercial lipases from Rhizomucor miehei (Lipozyme RM IM) and Candida antarctica (Novozym 435) was investigated. Although the bioconversion yield (BY) for Lipozyme RM IM (24.7 %) was close to that for Novozym 435 (24.0 %), the initial enzyme activity was 6.3 μmol CLnC/g enzyme/min and 1.6 μmol CLnC/g enzyme/min, respectively. Lipozyme RM IM was subsequently selected for further investigation. The structural analyses of SL indicated that the major products were 1,3‐dicapryl‐2‐linolenyl glycerol (CLnC) and 1(3)‐capryl‐2,3(1)‐dilinolenyl glycerol (CLnLn). In order to optimize the BY, selected parameters were investigated. The experimental results showed that using hexane as the reaction medium, at an initial water activity ( a w ) of 0.06, 10 mg solid enzyme/mL, substrate molar ratio of TC to TLN of 6:1 and a reaction time of 9 h, resulted in the highest BY (73.2 %). Using the optimized conditions, the effects of TLN concentration and other selective parameters, including the denaturation of the enzyme, controlling the a w and the addition of silica gel, on the mass productivity ( P M ), enzymatic productivity ( P E ) and volumetric productivity ( P V ) of the interesterification reaction, were also investigated. |
author2 |
Natural Sciences and Engineering Research Council of Canada |
format |
Article in Journal/Newspaper |
author |
Bai, Shan Aziz, Sarya Khodadadi, Maryam Bou Mitri, Christelle St‐Louis, Richard Kermasha, Selim |
spellingShingle |
Bai, Shan Aziz, Sarya Khodadadi, Maryam Bou Mitri, Christelle St‐Louis, Richard Kermasha, Selim Lipase‐Catalyzed Synthesis of Medium‐Long‐Medium Type Structured Lipids Using Tricaprylin and Trilinolenin as Substrate Models |
author_facet |
Bai, Shan Aziz, Sarya Khodadadi, Maryam Bou Mitri, Christelle St‐Louis, Richard Kermasha, Selim |
author_sort |
Bai, Shan |
title |
Lipase‐Catalyzed Synthesis of Medium‐Long‐Medium Type Structured Lipids Using Tricaprylin and Trilinolenin as Substrate Models |
title_short |
Lipase‐Catalyzed Synthesis of Medium‐Long‐Medium Type Structured Lipids Using Tricaprylin and Trilinolenin as Substrate Models |
title_full |
Lipase‐Catalyzed Synthesis of Medium‐Long‐Medium Type Structured Lipids Using Tricaprylin and Trilinolenin as Substrate Models |
title_fullStr |
Lipase‐Catalyzed Synthesis of Medium‐Long‐Medium Type Structured Lipids Using Tricaprylin and Trilinolenin as Substrate Models |
title_full_unstemmed |
Lipase‐Catalyzed Synthesis of Medium‐Long‐Medium Type Structured Lipids Using Tricaprylin and Trilinolenin as Substrate Models |
title_sort |
lipase‐catalyzed synthesis of medium‐long‐medium type structured lipids using tricaprylin and trilinolenin as substrate models |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1007/s11746-012-2185-9 https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-012-2185-9 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 90, issue 3, page 377-389 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11746-012-2185-9 |
container_title |
Journal of the American Oil Chemists' Society |
container_volume |
90 |
container_issue |
3 |
container_start_page |
377 |
op_container_end_page |
389 |
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1800739466196811776 |