Lipase Immobilized Methacrylate Polymer Monolith Microreactor for Lipid Transformations and Online Analytics
Abstract This communication entails development of a facile continuous flow lipase immobilized poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate (poly(GMA‐ co ‐EDMA)) monolith microreactor for application in lipid transformations. Candida antarctica lipase B was covalently immobilized on poly(...
Published in: | Journal of the American Oil Chemists' Society |
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crwiley:10.1007/s11746-012-2139-2 2024-06-02T07:58:26+00:00 Lipase Immobilized Methacrylate Polymer Monolith Microreactor for Lipid Transformations and Online Analytics Mugo, Samuel M. Ayton, Karl 2012 http://dx.doi.org/10.1007/s11746-012-2139-2 https://onlinelibrary.wiley.com/doi/pdf/10.1007/s11746-012-2139-2 https://onlinelibrary.wiley.com/doi/full-xml/10.1007/s11746-012-2139-2 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 90, issue 1, page 65-72 ISSN 0003-021X 1558-9331 journal-article 2012 crwiley https://doi.org/10.1007/s11746-012-2139-2 2024-05-03T10:46:02Z Abstract This communication entails development of a facile continuous flow lipase immobilized poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate (poly(GMA‐ co ‐EDMA)) monolith microreactor for application in lipid transformations. Candida antarctica lipase B was covalently immobilized on poly(GMA‐ co ‐EDMA) monolith prepared in a 700‐μm (internal diameter) silica capillary. The specific activity of the immobilized lipase was calculated to be 30 ± 0.03 U/mg, where U refers to μg p ‐nitrophenol generated/min from 3.5 mM p ‐nitrophenyl butyrate solution. The microreactor performance was further tested for synthesis of lauryl laurate via esterification. Conversions of up to 97 % were realized at a flow rate of 10 μL/min of a mixture of 0.1 M in both lauric acid and lauryl alcohol. These microreactors could be reused at least 15 times over a 1 month time period, stored at room temperature, with minimal to no reduction in the activity of the enzyme. We have also demonstrated microreactors to be useful for facile transesterification of castor oil triglycerides with online ESI–MS analytics key in lipidomics applications. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Monolith ENVELOPE(163.283,163.283,-66.950,-66.950) Journal of the American Oil Chemists' Society 90 1 65 72 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract This communication entails development of a facile continuous flow lipase immobilized poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate (poly(GMA‐ co ‐EDMA)) monolith microreactor for application in lipid transformations. Candida antarctica lipase B was covalently immobilized on poly(GMA‐ co ‐EDMA) monolith prepared in a 700‐μm (internal diameter) silica capillary. The specific activity of the immobilized lipase was calculated to be 30 ± 0.03 U/mg, where U refers to μg p ‐nitrophenol generated/min from 3.5 mM p ‐nitrophenyl butyrate solution. The microreactor performance was further tested for synthesis of lauryl laurate via esterification. Conversions of up to 97 % were realized at a flow rate of 10 μL/min of a mixture of 0.1 M in both lauric acid and lauryl alcohol. These microreactors could be reused at least 15 times over a 1 month time period, stored at room temperature, with minimal to no reduction in the activity of the enzyme. We have also demonstrated microreactors to be useful for facile transesterification of castor oil triglycerides with online ESI–MS analytics key in lipidomics applications. |
format |
Article in Journal/Newspaper |
author |
Mugo, Samuel M. Ayton, Karl |
spellingShingle |
Mugo, Samuel M. Ayton, Karl Lipase Immobilized Methacrylate Polymer Monolith Microreactor for Lipid Transformations and Online Analytics |
author_facet |
Mugo, Samuel M. Ayton, Karl |
author_sort |
Mugo, Samuel M. |
title |
Lipase Immobilized Methacrylate Polymer Monolith Microreactor for Lipid Transformations and Online Analytics |
title_short |
Lipase Immobilized Methacrylate Polymer Monolith Microreactor for Lipid Transformations and Online Analytics |
title_full |
Lipase Immobilized Methacrylate Polymer Monolith Microreactor for Lipid Transformations and Online Analytics |
title_fullStr |
Lipase Immobilized Methacrylate Polymer Monolith Microreactor for Lipid Transformations and Online Analytics |
title_full_unstemmed |
Lipase Immobilized Methacrylate Polymer Monolith Microreactor for Lipid Transformations and Online Analytics |
title_sort |
lipase immobilized methacrylate polymer monolith microreactor for lipid transformations and online analytics |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1007/s11746-012-2139-2 https://onlinelibrary.wiley.com/doi/pdf/10.1007/s11746-012-2139-2 https://onlinelibrary.wiley.com/doi/full-xml/10.1007/s11746-012-2139-2 |
long_lat |
ENVELOPE(163.283,163.283,-66.950,-66.950) |
geographic |
Monolith |
geographic_facet |
Monolith |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 90, issue 1, page 65-72 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11746-012-2139-2 |
container_title |
Journal of the American Oil Chemists' Society |
container_volume |
90 |
container_issue |
1 |
container_start_page |
65 |
op_container_end_page |
72 |
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1800741782310354944 |