Bioimprinted Immobilization of Candida antarctica Lipase A for Concentration of Omega‐3 Polyunsaturated Fatty Acids
Abstract Bioimprinting and immobilization of Candida antarctica lipase A (CALA) was optimized by response surface methodology to improve its selectivity for concentration of omega‐3 polyunsaturated fatty acids (PUFA) by lipase‐catalyzed ethanolysis of salmon oil obtained from salmon by‐products. Enz...
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crwiley:10.1007/s11746-012-2090-2 2024-06-23T07:47:13+00:00 Bioimprinted Immobilization of Candida antarctica Lipase A for Concentration of Omega‐3 Polyunsaturated Fatty Acids Kahveci, Derya Xu, Xuebing 2012 http://dx.doi.org/10.1007/s11746-012-2090-2 https://onlinelibrary.wiley.com/doi/pdf/10.1007/s11746-012-2090-2 https://onlinelibrary.wiley.com/doi/full-xml/10.1007/s11746-012-2090-2 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 89, issue 10, page 1839-1845 ISSN 0003-021X 1558-9331 journal-article 2012 crwiley https://doi.org/10.1007/s11746-012-2090-2 2024-06-04T06:43:23Z Abstract Bioimprinting and immobilization of Candida antarctica lipase A (CALA) was optimized by response surface methodology to improve its selectivity for concentration of omega‐3 polyunsaturated fatty acids (PUFA) by lipase‐catalyzed ethanolysis of salmon oil obtained from salmon by‐products. Enzyme‐to‐support ratio, oleic acid concentration, and immobilization time were the factors investigated. Total omega‐3 PUFA lost to the ethyl esters (EE) fraction was monitored in relation to the varying levels of the factors. Results showed that the second‐order polynomial model generated adequately fitted the experimental data. The most critical factor was the enzyme‐to‐support ratio, which led to decreased recovery of omega‐3 PUFA in the glycerides fraction with increasing levels of enzyme amount. Optimum conditions were selected as follows: enzyme‐to‐support ratio, 0.2 ( w/w ); oleic acid concentration, 50 mM; immobilization time, 85 min. The total omega‐3 PUFA lost to the EE fraction through ethanolysis of salmon oil decreased from 3.76 to 1.47 (mol%) under the optimum conditions. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 89 10 1839 1845 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Bioimprinting and immobilization of Candida antarctica lipase A (CALA) was optimized by response surface methodology to improve its selectivity for concentration of omega‐3 polyunsaturated fatty acids (PUFA) by lipase‐catalyzed ethanolysis of salmon oil obtained from salmon by‐products. Enzyme‐to‐support ratio, oleic acid concentration, and immobilization time were the factors investigated. Total omega‐3 PUFA lost to the ethyl esters (EE) fraction was monitored in relation to the varying levels of the factors. Results showed that the second‐order polynomial model generated adequately fitted the experimental data. The most critical factor was the enzyme‐to‐support ratio, which led to decreased recovery of omega‐3 PUFA in the glycerides fraction with increasing levels of enzyme amount. Optimum conditions were selected as follows: enzyme‐to‐support ratio, 0.2 ( w/w ); oleic acid concentration, 50 mM; immobilization time, 85 min. The total omega‐3 PUFA lost to the EE fraction through ethanolysis of salmon oil decreased from 3.76 to 1.47 (mol%) under the optimum conditions. |
format |
Article in Journal/Newspaper |
author |
Kahveci, Derya Xu, Xuebing |
spellingShingle |
Kahveci, Derya Xu, Xuebing Bioimprinted Immobilization of Candida antarctica Lipase A for Concentration of Omega‐3 Polyunsaturated Fatty Acids |
author_facet |
Kahveci, Derya Xu, Xuebing |
author_sort |
Kahveci, Derya |
title |
Bioimprinted Immobilization of Candida antarctica Lipase A for Concentration of Omega‐3 Polyunsaturated Fatty Acids |
title_short |
Bioimprinted Immobilization of Candida antarctica Lipase A for Concentration of Omega‐3 Polyunsaturated Fatty Acids |
title_full |
Bioimprinted Immobilization of Candida antarctica Lipase A for Concentration of Omega‐3 Polyunsaturated Fatty Acids |
title_fullStr |
Bioimprinted Immobilization of Candida antarctica Lipase A for Concentration of Omega‐3 Polyunsaturated Fatty Acids |
title_full_unstemmed |
Bioimprinted Immobilization of Candida antarctica Lipase A for Concentration of Omega‐3 Polyunsaturated Fatty Acids |
title_sort |
bioimprinted immobilization of candida antarctica lipase a for concentration of omega‐3 polyunsaturated fatty acids |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1007/s11746-012-2090-2 https://onlinelibrary.wiley.com/doi/pdf/10.1007/s11746-012-2090-2 https://onlinelibrary.wiley.com/doi/full-xml/10.1007/s11746-012-2090-2 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 89, issue 10, page 1839-1845 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11746-012-2090-2 |
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Journal of the American Oil Chemists' Society |
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89 |
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10 |
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1839 |
op_container_end_page |
1845 |
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1802651305558147072 |