Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures

Abstract The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80–100 °C to measure the residual activity as a function of time. The glycerol‐containing water stabilized the immobilized CALB, especially at 30–60 wt% water contents...

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Published in:Journal of the American Oil Chemists' Society
Main Authors: Kobayashi, Takashi, Matsuo, Takemasa, Kimura, Yukitaka, Adachi, Shuji
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2008
Subjects:
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.1007/s11746-008-1285-z
https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-008-1285-z
id crwiley:10.1007/s11746-008-1285-z
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spelling crwiley:10.1007/s11746-008-1285-z 2024-09-09T19:10:25+00:00 Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures Kobayashi, Takashi Matsuo, Takemasa Kimura, Yukitaka Adachi, Shuji 2008 http://dx.doi.org/10.1007/s11746-008-1285-z https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-008-1285-z en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 85, issue 11, page 1041-1044 ISSN 0003-021X 1558-9331 journal-article 2008 crwiley https://doi.org/10.1007/s11746-008-1285-z 2024-07-09T04:15:01Z Abstract The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80–100 °C to measure the residual activity as a function of time. The glycerol‐containing water stabilized the immobilized CALB, especially at 30–60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 85 11 1041 1044
institution Open Polar
collection Wiley Online Library
op_collection_id crwiley
language English
description Abstract The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80–100 °C to measure the residual activity as a function of time. The glycerol‐containing water stabilized the immobilized CALB, especially at 30–60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution.
format Article in Journal/Newspaper
author Kobayashi, Takashi
Matsuo, Takemasa
Kimura, Yukitaka
Adachi, Shuji
spellingShingle Kobayashi, Takashi
Matsuo, Takemasa
Kimura, Yukitaka
Adachi, Shuji
Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures
author_facet Kobayashi, Takashi
Matsuo, Takemasa
Kimura, Yukitaka
Adachi, Shuji
author_sort Kobayashi, Takashi
title Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures
title_short Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures
title_full Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures
title_fullStr Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures
title_full_unstemmed Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures
title_sort thermal stability of immobilized lipase from candida antarctica in glycerols with various water contents at elevated temperatures
publisher Wiley
publishDate 2008
url http://dx.doi.org/10.1007/s11746-008-1285-z
https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-008-1285-z
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Journal of the American Oil Chemists' Society
volume 85, issue 11, page 1041-1044
ISSN 0003-021X 1558-9331
op_rights http://onlinelibrary.wiley.com/termsAndConditions#vor
op_doi https://doi.org/10.1007/s11746-008-1285-z
container_title Journal of the American Oil Chemists' Society
container_volume 85
container_issue 11
container_start_page 1041
op_container_end_page 1044
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