FA selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media
Abstract FA reaction selectivity of Burkholderia cepacia, Rhizomucor miehei , and Candida antarctica fraction B lipases was compared between acyl‐transfer and esterification reactions. Multicompetitive reaction mixtures containing a series of n ‐chain FA (a C 4 –C 18 series; and a C 18∶x series, whe...
Published in: | Journal of the American Oil Chemists' Society |
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crwiley:10.1007/s11746-003-0682-4 2024-06-02T07:57:06+00:00 FA selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media Lee, Chen‐Hsien Parkin, Kirk L. 2003 http://dx.doi.org/10.1007/s11746-003-0682-4 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1007%2Fs11746-003-0682-4 https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-003-0682-4 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 80, issue 3 ISSN 0003-021X 1558-9331 journal-article 2003 crwiley https://doi.org/10.1007/s11746-003-0682-4 2024-05-03T11:09:21Z Abstract FA reaction selectivity of Burkholderia cepacia, Rhizomucor miehei , and Candida antarctica fraction B lipases was compared between acyl‐transfer and esterification reactions. Multicompetitive reaction mixtures containing a series of n ‐chain FA (a C 4 –C 18 series; and a C 18∶x series, where X=0‐3 double bonds) and a single acetate ester co‐substrate [triacetin, 1,2‐propanediol (1,2‐PD)diacetate, and 1,3‐PD diacetate] were studied in tert ‐butyl methyl ether at an a w of 0.69. For B. cepacia lipase, FA optima for C 8 , C 16 , and C 18∶2 were observed in all reactions with 1.0‐ to 5.9‐fold differences in FA selectivity. For R. miehei lipase, an optimum for C 8 FA was observed in all reactions with 1.2‐ to 6.7‐fold differences in FA selectivity. For C. antarctica lipase, FA optima for C 8 /C 10 were observed in all reactions with 1.0‐ to 2.8‐fold differences in FA selectivity. FA selectivities were broadly modulated upon changing from free polyol to acetate ester co‐substrates for B. cepacia and R miehei lipases, whereas FA selectivity modulations were more specific upon this change in reaction configuration for C. antarctica B lipase. For all lipases, reactivity toward unsaturated C 18∶x FA was enhanced in acyl‐transfer relative to esterification reactions with these polyol co‐substrates. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 80 3 |
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Wiley Online Library |
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language |
English |
description |
Abstract FA reaction selectivity of Burkholderia cepacia, Rhizomucor miehei , and Candida antarctica fraction B lipases was compared between acyl‐transfer and esterification reactions. Multicompetitive reaction mixtures containing a series of n ‐chain FA (a C 4 –C 18 series; and a C 18∶x series, where X=0‐3 double bonds) and a single acetate ester co‐substrate [triacetin, 1,2‐propanediol (1,2‐PD)diacetate, and 1,3‐PD diacetate] were studied in tert ‐butyl methyl ether at an a w of 0.69. For B. cepacia lipase, FA optima for C 8 , C 16 , and C 18∶2 were observed in all reactions with 1.0‐ to 5.9‐fold differences in FA selectivity. For R. miehei lipase, an optimum for C 8 FA was observed in all reactions with 1.2‐ to 6.7‐fold differences in FA selectivity. For C. antarctica lipase, FA optima for C 8 /C 10 were observed in all reactions with 1.0‐ to 2.8‐fold differences in FA selectivity. FA selectivities were broadly modulated upon changing from free polyol to acetate ester co‐substrates for B. cepacia and R miehei lipases, whereas FA selectivity modulations were more specific upon this change in reaction configuration for C. antarctica B lipase. For all lipases, reactivity toward unsaturated C 18∶x FA was enhanced in acyl‐transfer relative to esterification reactions with these polyol co‐substrates. |
format |
Article in Journal/Newspaper |
author |
Lee, Chen‐Hsien Parkin, Kirk L. |
spellingShingle |
Lee, Chen‐Hsien Parkin, Kirk L. FA selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media |
author_facet |
Lee, Chen‐Hsien Parkin, Kirk L. |
author_sort |
Lee, Chen‐Hsien |
title |
FA selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media |
title_short |
FA selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media |
title_full |
FA selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media |
title_fullStr |
FA selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media |
title_full_unstemmed |
FA selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media |
title_sort |
fa selectivity of lipases in acyl‐transfer reactions with acetate esters of polyols in organic media |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1007/s11746-003-0682-4 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1007%2Fs11746-003-0682-4 https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-003-0682-4 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 80, issue 3 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11746-003-0682-4 |
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Journal of the American Oil Chemists' Society |
container_volume |
80 |
container_issue |
3 |
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1800738645757394944 |