The use of immobilized Candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds
Abstract cis ‐Vaccenate was determined in milkweed ( Ascelpias ) seed employing supercritical fluid extraction (SFE) with in ‐situ enzymatic methanolysis. Candida antarctica lipase type B immobilized onto two different types of carrier materials—a hydrophobic polymer (Novozyme 435) and a silica‐base...
Published in: | Journal of the American Oil Chemists' Society |
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crwiley:10.1007/s11746-002-0508-4 2024-06-02T07:57:48+00:00 The use of immobilized Candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds Turner, Charlotta McKeon, Thomas 2002 http://dx.doi.org/10.1007/s11746-002-0508-4 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1007%2Fs11746-002-0508-4 https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-002-0508-4 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 79, issue 5, page 473-478 ISSN 0003-021X 1558-9331 journal-article 2002 crwiley https://doi.org/10.1007/s11746-002-0508-4 2024-05-03T11:51:01Z Abstract cis ‐Vaccenate was determined in milkweed ( Ascelpias ) seed employing supercritical fluid extraction (SFE) with in ‐situ enzymatic methanolysis. Candida antarctica lipase type B immobilized onto two different types of carrier materials—a hydrophobic polymer (Novozyme 435) and a silica‐based material (NovoSample 40013)—was investigated. Several SFE parameters were investigated to find the optimal conditions, including extraction time, temperature, methanol concentration, and water level. It was shown that 90 min of dynamic extraction/reaction time was required, and that the optimal methanol and water concentrations differed for the two enzyme preparations studied. Overall, Novozyme 435 demonstrated the fastest reaction kinetics and consistently gave the highest vaccenate yields. The optimized methodology was applied on 15 species of milkweed seeds, giving an average recovery of 105±7% when compared to results obtained using a conventional methodology. The average relative standard deviations were 8% for the enzyme‐based method and 4% for the reference method. The proposed methodology was faster, was less laborious, and consumed less organic solvent than the reference method. Hence, SFE with in‐situ enzymatic methanolysis is a promising methodology for the analysis of FA in oilseeds. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 79 5 473 478 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract cis ‐Vaccenate was determined in milkweed ( Ascelpias ) seed employing supercritical fluid extraction (SFE) with in ‐situ enzymatic methanolysis. Candida antarctica lipase type B immobilized onto two different types of carrier materials—a hydrophobic polymer (Novozyme 435) and a silica‐based material (NovoSample 40013)—was investigated. Several SFE parameters were investigated to find the optimal conditions, including extraction time, temperature, methanol concentration, and water level. It was shown that 90 min of dynamic extraction/reaction time was required, and that the optimal methanol and water concentrations differed for the two enzyme preparations studied. Overall, Novozyme 435 demonstrated the fastest reaction kinetics and consistently gave the highest vaccenate yields. The optimized methodology was applied on 15 species of milkweed seeds, giving an average recovery of 105±7% when compared to results obtained using a conventional methodology. The average relative standard deviations were 8% for the enzyme‐based method and 4% for the reference method. The proposed methodology was faster, was less laborious, and consumed less organic solvent than the reference method. Hence, SFE with in‐situ enzymatic methanolysis is a promising methodology for the analysis of FA in oilseeds. |
format |
Article in Journal/Newspaper |
author |
Turner, Charlotta McKeon, Thomas |
spellingShingle |
Turner, Charlotta McKeon, Thomas The use of immobilized Candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds |
author_facet |
Turner, Charlotta McKeon, Thomas |
author_sort |
Turner, Charlotta |
title |
The use of immobilized Candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds |
title_short |
The use of immobilized Candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds |
title_full |
The use of immobilized Candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds |
title_fullStr |
The use of immobilized Candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds |
title_full_unstemmed |
The use of immobilized Candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds |
title_sort |
use of immobilized candida antarctica lipase for simultaneous supercritical fluid extraction and in‐situ methanolysis of cis‐vaccenic acid in milkweed seeds |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1007/s11746-002-0508-4 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1007%2Fs11746-002-0508-4 https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-002-0508-4 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 79, issue 5, page 473-478 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11746-002-0508-4 |
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Journal of the American Oil Chemists' Society |
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79 |
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5 |
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473 |
op_container_end_page |
478 |
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1800740993356529664 |