Lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin
Abstract Three commercially available immobilized lipases, Novozym 435 from Candida antarctica , Lipozyme IM from Rhizomucor miehei , and Lipase PS‐C from Pseudomonas cepacia , were used as biocatalysts for the interesterification of conjugated linoleic acid (CLA) ethyl ester and tricaprylin. The re...
Published in: | Journal of the American Oil Chemists' Society |
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crwiley:10.1007/s11746-001-0301-4 2024-06-02T07:58:31+00:00 Lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin Kim, In‐Hwan Yoon, Chil‐Surk Cho, Soo‐Hee Lee, Kwang‐Won Chung, Soo‐Hyun Tae, Beom‐Seok 2001 http://dx.doi.org/10.1007/s11746-001-0301-4 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1007%2Fs11746-001-0301-4 https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-001-0301-4 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 78, issue 5, page 547-551 ISSN 0003-021X 1558-9331 journal-article 2001 crwiley https://doi.org/10.1007/s11746-001-0301-4 2024-05-03T10:49:21Z Abstract Three commercially available immobilized lipases, Novozym 435 from Candida antarctica , Lipozyme IM from Rhizomucor miehei , and Lipase PS‐C from Pseudomonas cepacia , were used as biocatalysts for the interesterification of conjugated linoleic acid (CLA) ethyl ester and tricaprylin. The reactions were carried out in hexane, and the products were analyzed by gas‐liquid chromatography. The effects of molar ratio, enzyme load, incubation time, and temperature on CLA incorporation were investigated. Novozym 435, as compared to Lipozyme IM and Lipase PC‐C, showed the highest degree of CLA incorporation into tricaprylin. By hydrolysis with pancreatic lipase, it was found that Lipozyme IM and Lipase PS‐C exhibited high selectivity for the sn ‐1,3 position of the triacylglycerol early in the interesterification, with small extents of incorporation of CLA into the sn ‐2 position, probably due to acyl migration, at later reaction times. A small extent of sn ‐1,3 selectivity during interesterification by Novozym 435 was observed. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 78 5 547 551 |
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Wiley Online Library |
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language |
English |
description |
Abstract Three commercially available immobilized lipases, Novozym 435 from Candida antarctica , Lipozyme IM from Rhizomucor miehei , and Lipase PS‐C from Pseudomonas cepacia , were used as biocatalysts for the interesterification of conjugated linoleic acid (CLA) ethyl ester and tricaprylin. The reactions were carried out in hexane, and the products were analyzed by gas‐liquid chromatography. The effects of molar ratio, enzyme load, incubation time, and temperature on CLA incorporation were investigated. Novozym 435, as compared to Lipozyme IM and Lipase PC‐C, showed the highest degree of CLA incorporation into tricaprylin. By hydrolysis with pancreatic lipase, it was found that Lipozyme IM and Lipase PS‐C exhibited high selectivity for the sn ‐1,3 position of the triacylglycerol early in the interesterification, with small extents of incorporation of CLA into the sn ‐2 position, probably due to acyl migration, at later reaction times. A small extent of sn ‐1,3 selectivity during interesterification by Novozym 435 was observed. |
format |
Article in Journal/Newspaper |
author |
Kim, In‐Hwan Yoon, Chil‐Surk Cho, Soo‐Hee Lee, Kwang‐Won Chung, Soo‐Hyun Tae, Beom‐Seok |
spellingShingle |
Kim, In‐Hwan Yoon, Chil‐Surk Cho, Soo‐Hee Lee, Kwang‐Won Chung, Soo‐Hyun Tae, Beom‐Seok Lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin |
author_facet |
Kim, In‐Hwan Yoon, Chil‐Surk Cho, Soo‐Hee Lee, Kwang‐Won Chung, Soo‐Hyun Tae, Beom‐Seok |
author_sort |
Kim, In‐Hwan |
title |
Lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin |
title_short |
Lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin |
title_full |
Lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin |
title_fullStr |
Lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin |
title_full_unstemmed |
Lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin |
title_sort |
lipase‐catalyzed incorporation of conjugated linoleic acid into tricaprylin |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1007/s11746-001-0301-4 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1007%2Fs11746-001-0301-4 https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-001-0301-4 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 78, issue 5, page 547-551 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11746-001-0301-4 |
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Journal of the American Oil Chemists' Society |
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78 |
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5 |
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547 |
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551 |
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1800741890756182016 |