Production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: Optimization of parameters by response surface methodology
Abstract 6‐ O ‐β‐ D (+)‐Glucose stearate serving as a model product of glucose fatty acid monoesters was synthesized using lipase B from Candiada antarctica (Chirazyme ® L‐2) in a mainly solid‐phase system in a stirred‐tank membrane reactor. Esterification was performed in the presence of a small am...
Published in: | Journal of the American Oil Chemists' Society |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2001
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1007/s11746-001-0235-x https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-001-0235-x |
id |
crwiley:10.1007/s11746-001-0235-x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1007/s11746-001-0235-x 2024-09-15T17:44:25+00:00 Production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: Optimization of parameters by response surface methodology Yan, Youchun Bornscheuer, Uwe T. Stadler, Gerhard Lutz‐Wahl, Sabine Reuss, Matthias Schmid, Rolf D. 2001 http://dx.doi.org/10.1007/s11746-001-0235-x https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-001-0235-x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 78, issue 2, page 147-153 ISSN 0003-021X 1558-9331 journal-article 2001 crwiley https://doi.org/10.1007/s11746-001-0235-x 2024-08-09T04:30:09Z Abstract 6‐ O ‐β‐ D (+)‐Glucose stearate serving as a model product of glucose fatty acid monoesters was synthesized using lipase B from Candiada antarctica (Chirazyme ® L‐2) in a mainly solid‐phase system in a stirred‐tank membrane reactor. Esterification was performed in the presence of a small amount of ethyl methylketone (EMK), maintaining a catalytic liquid phase as well as forming an azeotrope with the reaction water. The azeotrope was evaporated and broken by membrane vapor permeation, then the dried EMK was returned to the reaction medium. The process was optimized by response surface methodology based on five major reaction parameters [time ( T ), substrate ratio (acyl donor to glucose, S r , temperature ( R t ), amount of solvent [solvent to substrates, [w/w], S a ), and enzyme load ( E l )] varied at three levels, resulting in higher yields. Thus, under optimized conditions [ T r =58 h; S r =2.7; E l =8.9% (w/w); R t =78°C; S a =1.9], up to 93% yields of glucose stearate were achieved. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 78 2 147 153 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract 6‐ O ‐β‐ D (+)‐Glucose stearate serving as a model product of glucose fatty acid monoesters was synthesized using lipase B from Candiada antarctica (Chirazyme ® L‐2) in a mainly solid‐phase system in a stirred‐tank membrane reactor. Esterification was performed in the presence of a small amount of ethyl methylketone (EMK), maintaining a catalytic liquid phase as well as forming an azeotrope with the reaction water. The azeotrope was evaporated and broken by membrane vapor permeation, then the dried EMK was returned to the reaction medium. The process was optimized by response surface methodology based on five major reaction parameters [time ( T ), substrate ratio (acyl donor to glucose, S r , temperature ( R t ), amount of solvent [solvent to substrates, [w/w], S a ), and enzyme load ( E l )] varied at three levels, resulting in higher yields. Thus, under optimized conditions [ T r =58 h; S r =2.7; E l =8.9% (w/w); R t =78°C; S a =1.9], up to 93% yields of glucose stearate were achieved. |
format |
Article in Journal/Newspaper |
author |
Yan, Youchun Bornscheuer, Uwe T. Stadler, Gerhard Lutz‐Wahl, Sabine Reuss, Matthias Schmid, Rolf D. |
spellingShingle |
Yan, Youchun Bornscheuer, Uwe T. Stadler, Gerhard Lutz‐Wahl, Sabine Reuss, Matthias Schmid, Rolf D. Production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: Optimization of parameters by response surface methodology |
author_facet |
Yan, Youchun Bornscheuer, Uwe T. Stadler, Gerhard Lutz‐Wahl, Sabine Reuss, Matthias Schmid, Rolf D. |
author_sort |
Yan, Youchun |
title |
Production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: Optimization of parameters by response surface methodology |
title_short |
Production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: Optimization of parameters by response surface methodology |
title_full |
Production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: Optimization of parameters by response surface methodology |
title_fullStr |
Production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: Optimization of parameters by response surface methodology |
title_full_unstemmed |
Production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: Optimization of parameters by response surface methodology |
title_sort |
production of sugar fatty acid estrs by enzymatic esterification in a stirred‐tank membrane reactor: optimization of parameters by response surface methodology |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1007/s11746-001-0235-x https://onlinelibrary.wiley.com/doi/full/10.1007/s11746-001-0235-x |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 78, issue 2, page 147-153 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11746-001-0235-x |
container_title |
Journal of the American Oil Chemists' Society |
container_volume |
78 |
container_issue |
2 |
container_start_page |
147 |
op_container_end_page |
153 |
_version_ |
1810491998101045248 |