Metabolism of n−3 and n−6 fatty acids in Atlantic salmon liver: Stimulation by essential fatty acid deficiency
Abstract Oxidation, esterification, desaturation, and elongation of [1‐ 14 C]18∶2n−6 and [1‐ 14 C]18∶3n−3 were studied using hepatocytes from Atlantic salmon ( Salmo salar I.) maintained on diets deficient in n−3 and n−6 polyunsaturated fatty acids (PUFA) or supplemented with n−3 PUFA. For both diet...
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crwiley:10.1007/s11745-999-0468-3 2024-09-15T17:56:06+00:00 Metabolism of n−3 and n−6 fatty acids in Atlantic salmon liver: Stimulation by essential fatty acid deficiency Ruyter, B. Thomassen, M. S. 1999 http://dx.doi.org/10.1007/s11745-999-0468-3 https://onlinelibrary.wiley.com/doi/full/10.1007/s11745-999-0468-3 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Lipids volume 34, issue 11, page 1167-1176 ISSN 0024-4201 1558-9307 journal-article 1999 crwiley https://doi.org/10.1007/s11745-999-0468-3 2024-09-05T05:07:57Z Abstract Oxidation, esterification, desaturation, and elongation of [1‐ 14 C]18∶2n−6 and [1‐ 14 C]18∶3n−3 were studied using hepatocytes from Atlantic salmon ( Salmo salar I.) maintained on diets deficient in n−3 and n−6 polyunsaturated fatty acids (PUFA) or supplemented with n−3 PUFA. For both dietary groups, radioactivity from 18∶3n−3 was incoporated into lipid fractions two to three times faster than from 18∶2n−6, and essential fatty acids (FFA) deficiency doubled the incorporation. Oxidation to CO 2 was very low and was independent of substrate or diet, whereas oxidation to acid‐soluble products was stimulated by EFA deficiency. Products from 18∶2n−6 were mainly 18∶3n−6, 20∶3n−6, and 20∶4n−6, with minor amounts of 20∶2n−6 and 22∶5n−6. Products from 18∶3n−3 were mainly 18∶4n−3, 20∶5n−3, and 22∶6n−3, with small amounts of 20∶3n−3. The percentage of 22∶6n−3 in the polar lipid fraction of EFA‐deficient hepatocytes was fourfold higher than in n−3 PUFA‐supplemented cells. This correlated well with our other results obtained after abdominal injection of [1‐ 14 C]18∶3n−3 and [1‐ 14 C]18∶2n−6. In hepatocytes incubated with [4,5‐ 3 H]‐22∶6n−3, 20∶5n−3 was the main product. This retrocon‐version was increased by EFA deficiency, as was peroxisomal β‐oxidation activity. This study shows that 18∶2n−6 and 18∶3n−3 can be elongated and desaturated in Atlantic salmon liver, and that this conversion and the activity of retroconversion of very long chain PUFA is markedly enhanced by FFA deficiency. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Lipids 34 11 1167 1176 |
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Wiley Online Library |
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English |
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Abstract Oxidation, esterification, desaturation, and elongation of [1‐ 14 C]18∶2n−6 and [1‐ 14 C]18∶3n−3 were studied using hepatocytes from Atlantic salmon ( Salmo salar I.) maintained on diets deficient in n−3 and n−6 polyunsaturated fatty acids (PUFA) or supplemented with n−3 PUFA. For both dietary groups, radioactivity from 18∶3n−3 was incoporated into lipid fractions two to three times faster than from 18∶2n−6, and essential fatty acids (FFA) deficiency doubled the incorporation. Oxidation to CO 2 was very low and was independent of substrate or diet, whereas oxidation to acid‐soluble products was stimulated by EFA deficiency. Products from 18∶2n−6 were mainly 18∶3n−6, 20∶3n−6, and 20∶4n−6, with minor amounts of 20∶2n−6 and 22∶5n−6. Products from 18∶3n−3 were mainly 18∶4n−3, 20∶5n−3, and 22∶6n−3, with small amounts of 20∶3n−3. The percentage of 22∶6n−3 in the polar lipid fraction of EFA‐deficient hepatocytes was fourfold higher than in n−3 PUFA‐supplemented cells. This correlated well with our other results obtained after abdominal injection of [1‐ 14 C]18∶3n−3 and [1‐ 14 C]18∶2n−6. In hepatocytes incubated with [4,5‐ 3 H]‐22∶6n−3, 20∶5n−3 was the main product. This retrocon‐version was increased by EFA deficiency, as was peroxisomal β‐oxidation activity. This study shows that 18∶2n−6 and 18∶3n−3 can be elongated and desaturated in Atlantic salmon liver, and that this conversion and the activity of retroconversion of very long chain PUFA is markedly enhanced by FFA deficiency. |
format |
Article in Journal/Newspaper |
author |
Ruyter, B. Thomassen, M. S. |
spellingShingle |
Ruyter, B. Thomassen, M. S. Metabolism of n−3 and n−6 fatty acids in Atlantic salmon liver: Stimulation by essential fatty acid deficiency |
author_facet |
Ruyter, B. Thomassen, M. S. |
author_sort |
Ruyter, B. |
title |
Metabolism of n−3 and n−6 fatty acids in Atlantic salmon liver: Stimulation by essential fatty acid deficiency |
title_short |
Metabolism of n−3 and n−6 fatty acids in Atlantic salmon liver: Stimulation by essential fatty acid deficiency |
title_full |
Metabolism of n−3 and n−6 fatty acids in Atlantic salmon liver: Stimulation by essential fatty acid deficiency |
title_fullStr |
Metabolism of n−3 and n−6 fatty acids in Atlantic salmon liver: Stimulation by essential fatty acid deficiency |
title_full_unstemmed |
Metabolism of n−3 and n−6 fatty acids in Atlantic salmon liver: Stimulation by essential fatty acid deficiency |
title_sort |
metabolism of n−3 and n−6 fatty acids in atlantic salmon liver: stimulation by essential fatty acid deficiency |
publisher |
Wiley |
publishDate |
1999 |
url |
http://dx.doi.org/10.1007/s11745-999-0468-3 https://onlinelibrary.wiley.com/doi/full/10.1007/s11745-999-0468-3 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Lipids volume 34, issue 11, page 1167-1176 ISSN 0024-4201 1558-9307 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11745-999-0468-3 |
container_title |
Lipids |
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34 |
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11 |
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1167 |
op_container_end_page |
1176 |
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1810432316567191552 |