Isolation and Characterization of a Stress‐Dependent Plastidial Δ 12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7
Abstract An acclimation to the changing physicochemical conditions and high amount of Δ 12 ‐unsaturated fatty acids of the Antarctic Chlorella vulgaris NJ‐7 prompted us to speculate about the involvement of Δ 12 ‐fatty acid desaturases (FAD) in its adaptation to the extremely unfavorable ambience. A...
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crwiley:10.1007/s11745-009-3381-8 2024-09-09T19:11:06+00:00 Isolation and Characterization of a Stress‐Dependent Plastidial Δ 12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7 Lu, Yandu Chi, Xiaoyuan Li, Zhaoxin Yang, Qingli Li, Fuchao Liu, Shaofang Gan, Qinhua Qin, Song National Natural Science Foundation of China 2010 http://dx.doi.org/10.1007/s11745-009-3381-8 https://onlinelibrary.wiley.com/doi/full/10.1007/s11745-009-3381-8 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Lipids volume 45, issue 2, page 179-187 ISSN 0024-4201 1558-9307 journal-article 2010 crwiley https://doi.org/10.1007/s11745-009-3381-8 2024-06-20T04:27:22Z Abstract An acclimation to the changing physicochemical conditions and high amount of Δ 12 ‐unsaturated fatty acids of the Antarctic Chlorella vulgaris NJ‐7 prompted us to speculate about the involvement of Δ 12 ‐fatty acid desaturases (FAD) in its adaptation to the extremely unfavorable ambience. A full‐length cDNA sequence, designated CvFAD6, was isolated from C. vulgaris NJ‐7 via RT‐PCR and RACE methods. Sequence alignment showed that the gene was homologous to corresponding Δ 12 ‐FAD from other eukaryotes. Phylogenetic analysis showed that it was grouped with plastidial Δ 12 ‐FAD with conserved histidine boxes. Yeast cells transformed with a plasmid construct containing CvFAD6 coding region accumulated a considerable amount of linoleic acid (18:2Δ 9,12 ), normally not present in wild‐type yeast cells, suggesting that the isolated gene encodes a functional Δ 12 enzyme. The correlation between the accumulation of CvFAD6 and temperature has been examined by real time PCR. The analysis showed a constant expression of CvFAD6 from 25 to 15 °C whereas a fourfold increased from 25 to 4 °C. Moreover, CvFAD6 transcription was more sensitive to saline stress since a 20‐fold increase at 6% NaCl was detected. Our data demonstrate that CvFAD6 is the enzyme responsible for the Δ 12 fatty acids desaturation involved in low temperature and high salinity acclimation for Antarctic C. vulgaris NJ‐7. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic Lipids 45 2 179 187 |
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Wiley Online Library |
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English |
description |
Abstract An acclimation to the changing physicochemical conditions and high amount of Δ 12 ‐unsaturated fatty acids of the Antarctic Chlorella vulgaris NJ‐7 prompted us to speculate about the involvement of Δ 12 ‐fatty acid desaturases (FAD) in its adaptation to the extremely unfavorable ambience. A full‐length cDNA sequence, designated CvFAD6, was isolated from C. vulgaris NJ‐7 via RT‐PCR and RACE methods. Sequence alignment showed that the gene was homologous to corresponding Δ 12 ‐FAD from other eukaryotes. Phylogenetic analysis showed that it was grouped with plastidial Δ 12 ‐FAD with conserved histidine boxes. Yeast cells transformed with a plasmid construct containing CvFAD6 coding region accumulated a considerable amount of linoleic acid (18:2Δ 9,12 ), normally not present in wild‐type yeast cells, suggesting that the isolated gene encodes a functional Δ 12 enzyme. The correlation between the accumulation of CvFAD6 and temperature has been examined by real time PCR. The analysis showed a constant expression of CvFAD6 from 25 to 15 °C whereas a fourfold increased from 25 to 4 °C. Moreover, CvFAD6 transcription was more sensitive to saline stress since a 20‐fold increase at 6% NaCl was detected. Our data demonstrate that CvFAD6 is the enzyme responsible for the Δ 12 fatty acids desaturation involved in low temperature and high salinity acclimation for Antarctic C. vulgaris NJ‐7. |
author2 |
National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Lu, Yandu Chi, Xiaoyuan Li, Zhaoxin Yang, Qingli Li, Fuchao Liu, Shaofang Gan, Qinhua Qin, Song |
spellingShingle |
Lu, Yandu Chi, Xiaoyuan Li, Zhaoxin Yang, Qingli Li, Fuchao Liu, Shaofang Gan, Qinhua Qin, Song Isolation and Characterization of a Stress‐Dependent Plastidial Δ 12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7 |
author_facet |
Lu, Yandu Chi, Xiaoyuan Li, Zhaoxin Yang, Qingli Li, Fuchao Liu, Shaofang Gan, Qinhua Qin, Song |
author_sort |
Lu, Yandu |
title |
Isolation and Characterization of a Stress‐Dependent Plastidial Δ 12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7 |
title_short |
Isolation and Characterization of a Stress‐Dependent Plastidial Δ 12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7 |
title_full |
Isolation and Characterization of a Stress‐Dependent Plastidial Δ 12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7 |
title_fullStr |
Isolation and Characterization of a Stress‐Dependent Plastidial Δ 12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7 |
title_full_unstemmed |
Isolation and Characterization of a Stress‐Dependent Plastidial Δ 12 Fatty Acid Desaturase from the Antarctic Microalga Chlorella vulgaris NJ‐7 |
title_sort |
isolation and characterization of a stress‐dependent plastidial δ 12 fatty acid desaturase from the antarctic microalga chlorella vulgaris nj‐7 |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1007/s11745-009-3381-8 https://onlinelibrary.wiley.com/doi/full/10.1007/s11745-009-3381-8 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Lipids volume 45, issue 2, page 179-187 ISSN 0024-4201 1558-9307 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11745-009-3381-8 |
container_title |
Lipids |
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45 |
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2 |
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179 |
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187 |
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1809826765902708736 |