Lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein
Abstract The objective of this study was to find the optimal parameters for lipase‐catalyzed methanolysis of triricinolein to produce 1,2(2,3)‐diricinolein. Four different immobilized lipases were tested, Candida antarctica type B (CALB), Rhizomucor miehei (RML), Pseudomonas cepacia (PCL), and Penic...
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crwiley:10.1007/s11745-003-1179-5 2024-06-02T07:58:14+00:00 Lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein Turner, Charlotta He, Xiaohua Nguyen, Tasha Lin, Jiann‐Tsyh Wong, Rosalind Y. Lundin, Robert E. Harden, Leslie McKeon, Thomas 2003 http://dx.doi.org/10.1007/s11745-003-1179-5 https://onlinelibrary.wiley.com/doi/full/10.1007/s11745-003-1179-5 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Lipids volume 38, issue 11, page 1197-1206 ISSN 0024-4201 1558-9307 journal-article 2003 crwiley https://doi.org/10.1007/s11745-003-1179-5 2024-05-03T11:47:08Z Abstract The objective of this study was to find the optimal parameters for lipase‐catalyzed methanolysis of triricinolein to produce 1,2(2,3)‐diricinolein. Four different immobilized lipases were tested, Candida antarctica type B (CALB), Rhizomucor miehei (RML), Pseudomonas cepacia (PCL), and Penicillium roquefortii (PRL). n ‐Hexane and diisopropyl ether (DIPE) were examined as reaction media at three different water activities ( a w ), 0.11, 0.53, and 0.97. The consumption of triricinolein and the formation of 1,2(2,3)‐diricinolein, methyl ricinoleate, and ricinoleic acid were followed for up to 48 h. PRL gave the highest yield of 1,2(2,3)‐diricinolein. Moreover, this lipase showed the highest specificity for the studied reaction, i.e., high selectivity for the reaction with triricinolein but low for 1,2(2,3)‐diricinolein. Recoveries of 93 and 88% DAG were obtained using PRL in DIPE at a w of 0.11 and 0.53, respectively. Further, NMR studies showed that a higher purity of the 1,2(2,3)‐isomer vs. the 1,3‐isomer was achieved at higher a w (88% at a w =0.53), compared to lower a w (71% at a w =0.11). The DAG obtained was acylated by the DAG acyltransferase from Arabidopsis thaliana . Therefore, this enzymatic product is a useful enzyme substrate for lipid biosynthesis. Accordingly, the use of PRL in DIPE at a w 0.53 is considered optimal for the synthesis of 1,2(2,3)‐diricinolein from triricinolein. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Lipids 38 11 1197 1206 |
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Wiley Online Library |
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English |
description |
Abstract The objective of this study was to find the optimal parameters for lipase‐catalyzed methanolysis of triricinolein to produce 1,2(2,3)‐diricinolein. Four different immobilized lipases were tested, Candida antarctica type B (CALB), Rhizomucor miehei (RML), Pseudomonas cepacia (PCL), and Penicillium roquefortii (PRL). n ‐Hexane and diisopropyl ether (DIPE) were examined as reaction media at three different water activities ( a w ), 0.11, 0.53, and 0.97. The consumption of triricinolein and the formation of 1,2(2,3)‐diricinolein, methyl ricinoleate, and ricinoleic acid were followed for up to 48 h. PRL gave the highest yield of 1,2(2,3)‐diricinolein. Moreover, this lipase showed the highest specificity for the studied reaction, i.e., high selectivity for the reaction with triricinolein but low for 1,2(2,3)‐diricinolein. Recoveries of 93 and 88% DAG were obtained using PRL in DIPE at a w of 0.11 and 0.53, respectively. Further, NMR studies showed that a higher purity of the 1,2(2,3)‐isomer vs. the 1,3‐isomer was achieved at higher a w (88% at a w =0.53), compared to lower a w (71% at a w =0.11). The DAG obtained was acylated by the DAG acyltransferase from Arabidopsis thaliana . Therefore, this enzymatic product is a useful enzyme substrate for lipid biosynthesis. Accordingly, the use of PRL in DIPE at a w 0.53 is considered optimal for the synthesis of 1,2(2,3)‐diricinolein from triricinolein. |
format |
Article in Journal/Newspaper |
author |
Turner, Charlotta He, Xiaohua Nguyen, Tasha Lin, Jiann‐Tsyh Wong, Rosalind Y. Lundin, Robert E. Harden, Leslie McKeon, Thomas |
spellingShingle |
Turner, Charlotta He, Xiaohua Nguyen, Tasha Lin, Jiann‐Tsyh Wong, Rosalind Y. Lundin, Robert E. Harden, Leslie McKeon, Thomas Lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein |
author_facet |
Turner, Charlotta He, Xiaohua Nguyen, Tasha Lin, Jiann‐Tsyh Wong, Rosalind Y. Lundin, Robert E. Harden, Leslie McKeon, Thomas |
author_sort |
Turner, Charlotta |
title |
Lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein |
title_short |
Lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein |
title_full |
Lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein |
title_fullStr |
Lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein |
title_full_unstemmed |
Lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein |
title_sort |
lipase‐catalyzed methanolysis of triricinolein in organic solvent to produce 1,2(2,3)‐diricinolein |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1007/s11745-003-1179-5 https://onlinelibrary.wiley.com/doi/full/10.1007/s11745-003-1179-5 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Lipids volume 38, issue 11, page 1197-1206 ISSN 0024-4201 1558-9307 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11745-003-1179-5 |
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Lipids |
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38 |
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11 |
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1197 |
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1206 |
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1800741519177547776 |