Molecular analysis of intact preen waxes of Calidris canutus (Aves: Scolopacidae) by gas chromatography/mass spectrometry
Abstract The intact preen wax esters of the red knot Calidris canutus were studied with gas chromatography/mass spectrometry (GC/MS) and GC/MS/MS. In this latter technique, transitions from the molecular ion to fragment ions representing the fatty acid moiety of the wax esters were measured, providi...
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crwiley:10.1007/s11745-000-553-7 2024-06-02T08:04:47+00:00 Molecular analysis of intact preen waxes of Calidris canutus (Aves: Scolopacidae) by gas chromatography/mass spectrometry Dekker, Marlèn H. A. Piersma, Theunis Sinninghe Damsté, Jaap S. 2000 http://dx.doi.org/10.1007/s11745-000-553-7 https://onlinelibrary.wiley.com/doi/full/10.1007/s11745-000-553-7 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Lipids volume 35, issue 5, page 533-541 ISSN 0024-4201 1558-9307 journal-article 2000 crwiley https://doi.org/10.1007/s11745-000-553-7 2024-05-03T11:02:38Z Abstract The intact preen wax esters of the red knot Calidris canutus were studied with gas chromatography/mass spectrometry (GC/MS) and GC/MS/MS. In this latter technique, transitions from the molecular ion to fragment ions representing the fatty acid moiety of the wax esters were measured, providing additional resolution to the analysis of wax esters. The C 21 −C 32 wax esters are composed of complex mixtures of hundreds of individual isomers. The odd carbon‐numbered wax esters are predominantly composed of even carbon‐numbered n ‐alcohols (C 14 , C 16 , and C 18 ) esterified predominantly with odd carbon‐numbered 2‐methyl fatty acids (C 7 , C 9 , C 11 , and C 13 ), resulting in relatively simple distributions. The even carbon‐numbered wax esters show a far more complex distribution due to a number of factors: (i) Their n ‐alcohol moieties are not dominated by even carbon‐numbered n ‐alcohol moieties are not dominated by even carbon‐numbered n ‐alcohols esterified with odd carbon‐numbered 2‐methyl fatty acids, but odd and even carbon‐numbered n ‐alcohols participate in approximately equal amounts; (ii) odd carbon‐numbered methyl‐branched alcohols participate abundantly in these wax ester clusters; and (iii) with increasing molecular weight, various isomers of the 2,6‐, 2,8‐, and 2,10‐dimethyl branched fatty acids also participate in the even carbon‐numbered wax esters. The data demonstrate that there is a clear biosynthetic control on the wax ester composition although the reasons for the complex chemistry of the waxes are not yet understood. Article in Journal/Newspaper Calidris canutus Red Knot Wiley Online Library Lipids 35 5 533 541 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The intact preen wax esters of the red knot Calidris canutus were studied with gas chromatography/mass spectrometry (GC/MS) and GC/MS/MS. In this latter technique, transitions from the molecular ion to fragment ions representing the fatty acid moiety of the wax esters were measured, providing additional resolution to the analysis of wax esters. The C 21 −C 32 wax esters are composed of complex mixtures of hundreds of individual isomers. The odd carbon‐numbered wax esters are predominantly composed of even carbon‐numbered n ‐alcohols (C 14 , C 16 , and C 18 ) esterified predominantly with odd carbon‐numbered 2‐methyl fatty acids (C 7 , C 9 , C 11 , and C 13 ), resulting in relatively simple distributions. The even carbon‐numbered wax esters show a far more complex distribution due to a number of factors: (i) Their n ‐alcohol moieties are not dominated by even carbon‐numbered n ‐alcohol moieties are not dominated by even carbon‐numbered n ‐alcohols esterified with odd carbon‐numbered 2‐methyl fatty acids, but odd and even carbon‐numbered n ‐alcohols participate in approximately equal amounts; (ii) odd carbon‐numbered methyl‐branched alcohols participate abundantly in these wax ester clusters; and (iii) with increasing molecular weight, various isomers of the 2,6‐, 2,8‐, and 2,10‐dimethyl branched fatty acids also participate in the even carbon‐numbered wax esters. The data demonstrate that there is a clear biosynthetic control on the wax ester composition although the reasons for the complex chemistry of the waxes are not yet understood. |
format |
Article in Journal/Newspaper |
author |
Dekker, Marlèn H. A. Piersma, Theunis Sinninghe Damsté, Jaap S. |
spellingShingle |
Dekker, Marlèn H. A. Piersma, Theunis Sinninghe Damsté, Jaap S. Molecular analysis of intact preen waxes of Calidris canutus (Aves: Scolopacidae) by gas chromatography/mass spectrometry |
author_facet |
Dekker, Marlèn H. A. Piersma, Theunis Sinninghe Damsté, Jaap S. |
author_sort |
Dekker, Marlèn H. A. |
title |
Molecular analysis of intact preen waxes of Calidris canutus (Aves: Scolopacidae) by gas chromatography/mass spectrometry |
title_short |
Molecular analysis of intact preen waxes of Calidris canutus (Aves: Scolopacidae) by gas chromatography/mass spectrometry |
title_full |
Molecular analysis of intact preen waxes of Calidris canutus (Aves: Scolopacidae) by gas chromatography/mass spectrometry |
title_fullStr |
Molecular analysis of intact preen waxes of Calidris canutus (Aves: Scolopacidae) by gas chromatography/mass spectrometry |
title_full_unstemmed |
Molecular analysis of intact preen waxes of Calidris canutus (Aves: Scolopacidae) by gas chromatography/mass spectrometry |
title_sort |
molecular analysis of intact preen waxes of calidris canutus (aves: scolopacidae) by gas chromatography/mass spectrometry |
publisher |
Wiley |
publishDate |
2000 |
url |
http://dx.doi.org/10.1007/s11745-000-553-7 https://onlinelibrary.wiley.com/doi/full/10.1007/s11745-000-553-7 |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_source |
Lipids volume 35, issue 5, page 533-541 ISSN 0024-4201 1558-9307 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/s11745-000-553-7 |
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Lipids |
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35 |
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5 |
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533 |
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541 |
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1800749443227582464 |