Elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( Scophthalmus maximus) brain astroglial cells in primary culture
Abstract The origin of docosahexaenoic acid (DHA, 22∶6n−3) that accumulates in turbot brain during development was investigated by studying the incorporation and metabolism via the desaturase/elongase pathways of [1‐ 14 C]‐labelled polyunsaturated fatty acids (PUFA) in primary cultures of brain astr...
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crwiley:10.1007/bf02536309 2024-06-02T08:14:11+00:00 Elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( Scophthalmus maximus) brain astroglial cells in primary culture Tocher, Dauglas R. 1993 http://dx.doi.org/10.1007/bf02536309 https://onlinelibrary.wiley.com/doi/full/10.1007/BF02536309 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Lipids volume 28, issue 4, page 267-272 ISSN 0024-4201 1558-9307 journal-article 1993 crwiley https://doi.org/10.1007/bf02536309 2024-05-03T11:28:15Z Abstract The origin of docosahexaenoic acid (DHA, 22∶6n−3) that accumulates in turbot brain during development was investigated by studying the incorporation and metabolism via the desaturase/elongase pathways of [1‐ 14 C]‐labelled polyunsaturated fatty acids (PUFA) in primary cultures of brain astrocytic glial cells. There was little specificity evident in the total incorporation of PUFAs into the turbot astrocytes. However, specificity was apparent in the distribution of the various PUFAs among the individual lipid classes. In particular, there was very specific incorporation of [ 14 C]arachidonic acid (AA, 20∶4n−6) into phosphatidylinositol balanced by a lower incorporation of this acid into total diradyl glycerophosphocholines. [ 14 C]‐Linolenic acid (LNA, 18∶3n−3) and [ 14 C]eicosapentaenoic acid (EPA, 20∶5n−3) were metabolized via the desaturase/elongase pathways to a significantly greater extent than [ 14 C]linoleic acid (18∶2n−6) and [ 14 C]AA. The turbot astrocytes expressed very little Δ5 desaturase activity and only low levels of Δ4 desaturation activity. Although the percentages were small, approximately 4–5 times as much labelled DHA was produced from [ 14 C]EPA compared with [ 14 C]LNA. However, it was concluded that very little DHA in the turbot brain could result from the metabolism of LNA and EPA in astrocytic glial cells. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Lipids 28 4 267 272 |
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Wiley Online Library |
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English |
description |
Abstract The origin of docosahexaenoic acid (DHA, 22∶6n−3) that accumulates in turbot brain during development was investigated by studying the incorporation and metabolism via the desaturase/elongase pathways of [1‐ 14 C]‐labelled polyunsaturated fatty acids (PUFA) in primary cultures of brain astrocytic glial cells. There was little specificity evident in the total incorporation of PUFAs into the turbot astrocytes. However, specificity was apparent in the distribution of the various PUFAs among the individual lipid classes. In particular, there was very specific incorporation of [ 14 C]arachidonic acid (AA, 20∶4n−6) into phosphatidylinositol balanced by a lower incorporation of this acid into total diradyl glycerophosphocholines. [ 14 C]‐Linolenic acid (LNA, 18∶3n−3) and [ 14 C]eicosapentaenoic acid (EPA, 20∶5n−3) were metabolized via the desaturase/elongase pathways to a significantly greater extent than [ 14 C]linoleic acid (18∶2n−6) and [ 14 C]AA. The turbot astrocytes expressed very little Δ5 desaturase activity and only low levels of Δ4 desaturation activity. Although the percentages were small, approximately 4–5 times as much labelled DHA was produced from [ 14 C]EPA compared with [ 14 C]LNA. However, it was concluded that very little DHA in the turbot brain could result from the metabolism of LNA and EPA in astrocytic glial cells. |
format |
Article in Journal/Newspaper |
author |
Tocher, Dauglas R. |
spellingShingle |
Tocher, Dauglas R. Elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( Scophthalmus maximus) brain astroglial cells in primary culture |
author_facet |
Tocher, Dauglas R. |
author_sort |
Tocher, Dauglas R. |
title |
Elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( Scophthalmus maximus) brain astroglial cells in primary culture |
title_short |
Elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( Scophthalmus maximus) brain astroglial cells in primary culture |
title_full |
Elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( Scophthalmus maximus) brain astroglial cells in primary culture |
title_fullStr |
Elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( Scophthalmus maximus) brain astroglial cells in primary culture |
title_full_unstemmed |
Elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( Scophthalmus maximus) brain astroglial cells in primary culture |
title_sort |
elongation predominates over desaturation in the metabolism of 18∶3n−3 and 20∶5n−3 in turbot ( scophthalmus maximus) brain astroglial cells in primary culture |
publisher |
Wiley |
publishDate |
1993 |
url |
http://dx.doi.org/10.1007/bf02536309 https://onlinelibrary.wiley.com/doi/full/10.1007/BF02536309 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Lipids volume 28, issue 4, page 267-272 ISSN 0024-4201 1558-9307 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/bf02536309 |
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Lipids |
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28 |
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4 |
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267 |
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272 |
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1800737908051673088 |