Enzymatic synthesis of structured lipids: Transesterification of triolein and caprylic acid ethyl ester
Abstract Structured lipids were successfully synthesized by lipase‐catalyzed transesterification (ester interchange) of caprylic acid ethyl ester and triolein. The transesterification reaction was carried out in organic solvent as reaction media. Eight commercially‐available lipases (10% w/w substra...
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crwiley:10.1007/bf02523903 2024-06-02T07:57:05+00:00 Enzymatic synthesis of structured lipids: Transesterification of triolein and caprylic acid ethyl ester Huang, Kuan‐Hsiang Akoh, Casimir C. 1996 http://dx.doi.org/10.1007/bf02523903 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1007%2FBF02523903 https://onlinelibrary.wiley.com/doi/full/10.1007/BF02523903 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the American Oil Chemists' Society volume 73, issue 2, page 245-250 ISSN 0003-021X 1558-9331 journal-article 1996 crwiley https://doi.org/10.1007/bf02523903 2024-05-03T11:30:55Z Abstract Structured lipids were successfully synthesized by lipase‐catalyzed transesterification (ester interchange) of caprylic acid ethyl ester and triolein. The transesterification reaction was carried out in organic solvent as reaction media. Eight commercially‐available lipases (10% w/w substrates) were screened for their ability to synthesize structured lipid by incubating with 100 mg triolein and 78.0 mg caprylic acid ethyl ester in 3 mL hexane at 45°C for 24 h. The products were analyzed by reverse‐phase high‐performance liquid chromatography with evaporative light‐scattering detector. Immobilized lipase IM60 from Rhizomucor miehei converted most triolein into structured lipids (41.7% dicapryloolein, 46.0% monocapryloolein, and 12.3% unreacted triolein). However, lipase SP435 from Candida antarctica had a higher activity at higher temperature. The reaction catalyzed by lipase SP435 yielded 62.0% dicapryloolein, 33.5% monocapryloolein, and 4.5% unreacted triolein at 55°C. Time course, incubation media, added water, and substrate concentration were also investigated in this study. The results suggest that lipase‐catalyzed transesterification of long‐chain triglycerides and medium‐chain fatty acid ethyl ester is feasible to synthesize structured lipids. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of the American Oil Chemists' Society 73 2 245 250 |
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Wiley Online Library |
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English |
description |
Abstract Structured lipids were successfully synthesized by lipase‐catalyzed transesterification (ester interchange) of caprylic acid ethyl ester and triolein. The transesterification reaction was carried out in organic solvent as reaction media. Eight commercially‐available lipases (10% w/w substrates) were screened for their ability to synthesize structured lipid by incubating with 100 mg triolein and 78.0 mg caprylic acid ethyl ester in 3 mL hexane at 45°C for 24 h. The products were analyzed by reverse‐phase high‐performance liquid chromatography with evaporative light‐scattering detector. Immobilized lipase IM60 from Rhizomucor miehei converted most triolein into structured lipids (41.7% dicapryloolein, 46.0% monocapryloolein, and 12.3% unreacted triolein). However, lipase SP435 from Candida antarctica had a higher activity at higher temperature. The reaction catalyzed by lipase SP435 yielded 62.0% dicapryloolein, 33.5% monocapryloolein, and 4.5% unreacted triolein at 55°C. Time course, incubation media, added water, and substrate concentration were also investigated in this study. The results suggest that lipase‐catalyzed transesterification of long‐chain triglycerides and medium‐chain fatty acid ethyl ester is feasible to synthesize structured lipids. |
format |
Article in Journal/Newspaper |
author |
Huang, Kuan‐Hsiang Akoh, Casimir C. |
spellingShingle |
Huang, Kuan‐Hsiang Akoh, Casimir C. Enzymatic synthesis of structured lipids: Transesterification of triolein and caprylic acid ethyl ester |
author_facet |
Huang, Kuan‐Hsiang Akoh, Casimir C. |
author_sort |
Huang, Kuan‐Hsiang |
title |
Enzymatic synthesis of structured lipids: Transesterification of triolein and caprylic acid ethyl ester |
title_short |
Enzymatic synthesis of structured lipids: Transesterification of triolein and caprylic acid ethyl ester |
title_full |
Enzymatic synthesis of structured lipids: Transesterification of triolein and caprylic acid ethyl ester |
title_fullStr |
Enzymatic synthesis of structured lipids: Transesterification of triolein and caprylic acid ethyl ester |
title_full_unstemmed |
Enzymatic synthesis of structured lipids: Transesterification of triolein and caprylic acid ethyl ester |
title_sort |
enzymatic synthesis of structured lipids: transesterification of triolein and caprylic acid ethyl ester |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1007/bf02523903 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1007%2FBF02523903 https://onlinelibrary.wiley.com/doi/full/10.1007/BF02523903 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of the American Oil Chemists' Society volume 73, issue 2, page 245-250 ISSN 0003-021X 1558-9331 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/bf02523903 |
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Journal of the American Oil Chemists' Society |
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73 |
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2 |
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245 |
op_container_end_page |
250 |
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1800738535877115904 |