Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution
Abstract Fluorescence anisotropy parameter of [p‐(6‐phenyl)‐1,3,5‐hexatrienyl]phenyl‐propionic acid (DPH‐PA) and 1‐(4‐trimethylammoniumphenyl)‐6‐phenyl‐1,3,5‐hexatriene (TMA‐DPH) embedded in synaptic plasma membranes prepared from brains of cold (5°C) and warm (22°C) adapted fish ( Cyprinus carpio L...
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crwiley:10.1007/bf02522461 2024-09-15T18:00:21+00:00 Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution Kitajka, K. Buda, Cs. Fodor, E. Halver, J. E. Farkas, T. 1996 http://dx.doi.org/10.1007/bf02522461 https://onlinelibrary.wiley.com/doi/full/10.1007/BF02522461 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Lipids volume 31, issue 10, page 1045-1050 ISSN 0024-4201 1558-9307 journal-article 1996 crwiley https://doi.org/10.1007/bf02522461 2024-07-23T04:10:28Z Abstract Fluorescence anisotropy parameter of [p‐(6‐phenyl)‐1,3,5‐hexatrienyl]phenyl‐propionic acid (DPH‐PA) and 1‐(4‐trimethylammoniumphenyl)‐6‐phenyl‐1,3,5‐hexatriene (TMA‐DPH) embedded in synaptic plasma membranes prepared from brains of cold (5°C) and warm (22°C) adapted fish ( Cyprinus carpio L.), rat ( Rattus norvegicus ) and bird ( Branta canadensis ), was studied. Fatty acid composition of total lipids as well as molecular species composition of diacyl phosphatidylcholines and phosphatidylethanolamines was also determined. The amount of long‐chain polyunsaturated fatty acids decreased with increasing body temperature. There was a nearcomplete compensation of membrane structural order for environmental/body tempeature over the evolutionary scale as seen by DPH‐PA. Using TMA‐DPH, the compensation was partial with rat and bird. Since DPH‐PA and TMA‐DPH differ in their charges, it is proposed, that the former reported membrane regions rich in cationic or zwitterionic (neutral) phospholipids and the latter, membrane regions rich in negatively charged phospholipids in the synaptic plasma membranes. Many different molecular species (20–25) of diacyl phosphatidylcholines and diacyl phosphatidylethanolamines were identified. The level of 16:0/22:6 phosphatidylcholine decreased while disaturated phosphatidylcholines increased with increase of environmental/body temperature from the fish through the bird. Level of 1‐monoenoic 2‐polyenoic phosphatidylethanolamines also decreased with an increase in environmental/body temperature. Experiments using vesicles made of mixed synthetic phosphatidylcholine vesicles (16:0/16:0, 16:0/18:1, 16:0/22:6 in various proportions) showed that increase in disaturated phosphatidylcholine species does not explain the observed complete adjustment of membrane structural order in synaptic plasma membranes. Change in level of 1‐monoenoic, 2‐polyenoic phosphatidylethanolamines might be one of the factors involved in controlling the biophysical properties of the membrane according to the ... Article in Journal/Newspaper Branta canadensis Wiley Online Library Lipids 31 10 1045 1050 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Fluorescence anisotropy parameter of [p‐(6‐phenyl)‐1,3,5‐hexatrienyl]phenyl‐propionic acid (DPH‐PA) and 1‐(4‐trimethylammoniumphenyl)‐6‐phenyl‐1,3,5‐hexatriene (TMA‐DPH) embedded in synaptic plasma membranes prepared from brains of cold (5°C) and warm (22°C) adapted fish ( Cyprinus carpio L.), rat ( Rattus norvegicus ) and bird ( Branta canadensis ), was studied. Fatty acid composition of total lipids as well as molecular species composition of diacyl phosphatidylcholines and phosphatidylethanolamines was also determined. The amount of long‐chain polyunsaturated fatty acids decreased with increasing body temperature. There was a nearcomplete compensation of membrane structural order for environmental/body tempeature over the evolutionary scale as seen by DPH‐PA. Using TMA‐DPH, the compensation was partial with rat and bird. Since DPH‐PA and TMA‐DPH differ in their charges, it is proposed, that the former reported membrane regions rich in cationic or zwitterionic (neutral) phospholipids and the latter, membrane regions rich in negatively charged phospholipids in the synaptic plasma membranes. Many different molecular species (20–25) of diacyl phosphatidylcholines and diacyl phosphatidylethanolamines were identified. The level of 16:0/22:6 phosphatidylcholine decreased while disaturated phosphatidylcholines increased with increase of environmental/body temperature from the fish through the bird. Level of 1‐monoenoic 2‐polyenoic phosphatidylethanolamines also decreased with an increase in environmental/body temperature. Experiments using vesicles made of mixed synthetic phosphatidylcholine vesicles (16:0/16:0, 16:0/18:1, 16:0/22:6 in various proportions) showed that increase in disaturated phosphatidylcholine species does not explain the observed complete adjustment of membrane structural order in synaptic plasma membranes. Change in level of 1‐monoenoic, 2‐polyenoic phosphatidylethanolamines might be one of the factors involved in controlling the biophysical properties of the membrane according to the ... |
format |
Article in Journal/Newspaper |
author |
Kitajka, K. Buda, Cs. Fodor, E. Halver, J. E. Farkas, T. |
spellingShingle |
Kitajka, K. Buda, Cs. Fodor, E. Halver, J. E. Farkas, T. Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution |
author_facet |
Kitajka, K. Buda, Cs. Fodor, E. Halver, J. E. Farkas, T. |
author_sort |
Kitajka, K. |
title |
Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution |
title_short |
Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution |
title_full |
Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution |
title_fullStr |
Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution |
title_full_unstemmed |
Involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution |
title_sort |
involvement of phospholipid molecular species in controlling structural order of vertebrate brain synaptic membranes during thermal evolution |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1007/bf02522461 https://onlinelibrary.wiley.com/doi/full/10.1007/BF02522461 |
genre |
Branta canadensis |
genre_facet |
Branta canadensis |
op_source |
Lipids volume 31, issue 10, page 1045-1050 ISSN 0024-4201 1558-9307 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1007/bf02522461 |
container_title |
Lipids |
container_volume |
31 |
container_issue |
10 |
container_start_page |
1045 |
op_container_end_page |
1050 |
_version_ |
1810437522279366656 |