Distribution of acetylated tubulin in cultured cells and tissues from the Atlantic cod ( Gadus morhua). Role of acetylation in cold adaptation and drug stability
Abstract The Atlantic cod (Gadus morhua) is a poikilothermic animal living at temperatures between 2‐15°C. Isolated cod brain tubulin is, in contrast to mammalian brain tubulin, posttranslationally modified by acetylation to a high extent. To investigate the role of acetylation in cold adaptation, m...
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Online Access: | http://dx.doi.org/10.1006/cbir.1995.1126 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1006%2Fcbir.1995.1126 https://onlinelibrary.wiley.com/doi/pdf/10.1006/cbir.1995.1126 |
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crwiley:10.1006/cbir.1995.1126 2024-06-02T08:03:09+00:00 Distribution of acetylated tubulin in cultured cells and tissues from the Atlantic cod ( Gadus morhua). Role of acetylation in cold adaptation and drug stability Rutberg, M. Billger, M. Modig, C. Wallin, M. 1995 http://dx.doi.org/10.1006/cbir.1995.1126 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1006%2Fcbir.1995.1126 https://onlinelibrary.wiley.com/doi/pdf/10.1006/cbir.1995.1126 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Cell Biology International volume 19, issue 9, page 749-758 ISSN 1065-6995 1095-8355 journal-article 1995 crwiley https://doi.org/10.1006/cbir.1995.1126 2024-05-03T11:02:52Z Abstract The Atlantic cod (Gadus morhua) is a poikilothermic animal living at temperatures between 2‐15°C. Isolated cod brain tubulin is, in contrast to mammalian brain tubulin, posttranslationally modified by acetylation to a high extent. To investigate the role of acetylation in cold adaptation, microtubules were isolated by a taxol‐dependent procedure from different organs of the cod, and cells from different tissues were cultured. All cells from skin and brain were able to grow between 4°C and room temperature. Microtubules in the cultured cells were sometimes severed near the periphery of the cells. Microtubules in brain cells were in general more stable to vinblastine and colchicine, when compared to skin cells. Acetylated microtubules were found only in brain cells, in peripheral nerves on scales and in nerves of the intestinal tract and in microtubules isolated from neuronal tissue. Our results show that acetylated microtubules are found both in the central and peripheral nervous system, but that there is no correlation between acetylation and cold‐adaptation. Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library Cell Biology International 19 9 749 758 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The Atlantic cod (Gadus morhua) is a poikilothermic animal living at temperatures between 2‐15°C. Isolated cod brain tubulin is, in contrast to mammalian brain tubulin, posttranslationally modified by acetylation to a high extent. To investigate the role of acetylation in cold adaptation, microtubules were isolated by a taxol‐dependent procedure from different organs of the cod, and cells from different tissues were cultured. All cells from skin and brain were able to grow between 4°C and room temperature. Microtubules in the cultured cells were sometimes severed near the periphery of the cells. Microtubules in brain cells were in general more stable to vinblastine and colchicine, when compared to skin cells. Acetylated microtubules were found only in brain cells, in peripheral nerves on scales and in nerves of the intestinal tract and in microtubules isolated from neuronal tissue. Our results show that acetylated microtubules are found both in the central and peripheral nervous system, but that there is no correlation between acetylation and cold‐adaptation. |
format |
Article in Journal/Newspaper |
author |
Rutberg, M. Billger, M. Modig, C. Wallin, M. |
spellingShingle |
Rutberg, M. Billger, M. Modig, C. Wallin, M. Distribution of acetylated tubulin in cultured cells and tissues from the Atlantic cod ( Gadus morhua). Role of acetylation in cold adaptation and drug stability |
author_facet |
Rutberg, M. Billger, M. Modig, C. Wallin, M. |
author_sort |
Rutberg, M. |
title |
Distribution of acetylated tubulin in cultured cells and tissues from the Atlantic cod ( Gadus morhua). Role of acetylation in cold adaptation and drug stability |
title_short |
Distribution of acetylated tubulin in cultured cells and tissues from the Atlantic cod ( Gadus morhua). Role of acetylation in cold adaptation and drug stability |
title_full |
Distribution of acetylated tubulin in cultured cells and tissues from the Atlantic cod ( Gadus morhua). Role of acetylation in cold adaptation and drug stability |
title_fullStr |
Distribution of acetylated tubulin in cultured cells and tissues from the Atlantic cod ( Gadus morhua). Role of acetylation in cold adaptation and drug stability |
title_full_unstemmed |
Distribution of acetylated tubulin in cultured cells and tissues from the Atlantic cod ( Gadus morhua). Role of acetylation in cold adaptation and drug stability |
title_sort |
distribution of acetylated tubulin in cultured cells and tissues from the atlantic cod ( gadus morhua). role of acetylation in cold adaptation and drug stability |
publisher |
Wiley |
publishDate |
1995 |
url |
http://dx.doi.org/10.1006/cbir.1995.1126 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1006%2Fcbir.1995.1126 https://onlinelibrary.wiley.com/doi/pdf/10.1006/cbir.1995.1126 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Cell Biology International volume 19, issue 9, page 749-758 ISSN 1065-6995 1095-8355 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1006/cbir.1995.1126 |
container_title |
Cell Biology International |
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19 |
container_issue |
9 |
container_start_page |
749 |
op_container_end_page |
758 |
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1800747615622529024 |