Sex Identification of Four Penguin Species Using Locus‐Specific PCR
Traditional methods for sex identification are not applicable to sexually monomorphic species, leading to difficulties in the management of their breeding programs. To identify sex in sexually monomorphic birds, molecular methods have been established. Two established primer pairs (2550F/2718R and p...
Published in: | Zoo Biology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2012
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/zoo.21005 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fzoo.21005 https://onlinelibrary.wiley.com/doi/pdf/10.1002/zoo.21005 |
id |
crwiley:10.1002/zoo.21005 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/zoo.21005 2024-06-02T08:09:52+00:00 Sex Identification of Four Penguin Species Using Locus‐Specific PCR Zhang, Peijun Han, Jiabo Liu, Quansheng Zhang, Junxin Zhang, Xianfeng 2012 http://dx.doi.org/10.1002/zoo.21005 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fzoo.21005 https://onlinelibrary.wiley.com/doi/pdf/10.1002/zoo.21005 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Zoo Biology volume 32, issue 3, page 257-261 ISSN 0733-3188 1098-2361 journal-article 2012 crwiley https://doi.org/10.1002/zoo.21005 2024-05-03T10:59:05Z Traditional methods for sex identification are not applicable to sexually monomorphic species, leading to difficulties in the management of their breeding programs. To identify sex in sexually monomorphic birds, molecular methods have been established. Two established primer pairs (2550F/2718R and p8/p2) amplify the CHD1 gene region from both the Z and W chromosomes. Here, we evaluated the use of these primers for sex identification in four sexually monomorphic penguin species: king penguins ( Aptenodytes patagonicus ), rockhopper penguins ( Eudyptes chrysocome ), gentoo penguins ( Pygoscelis papua ), and Magellanic penguins ( Spheniscus magellanicus ). For all species except rockhopper penguins, primer pair 2550F/2718R resulted in two distinct CHD1Z and CHD1W PCR bands, allowing for sex identification. For rockhopper penguins, only primer pair p8/p2 yielded different CHD1Z and CHD1W bands, which were faint and similar in size making them difficult to distinguish. As a result, we designed a new primer pair (PL/PR) that efficiently determined the gender of individuals from all four penguin species. Sequencing of the PCR products confirmed that they were from the CHD1 gene region. Primer pair PL/PR can be evaluated for use in sexing other penguin species, which will be crucial for the management of new penguin breeding programs. Zoo Biol 32:257–261, 2013. © 2012 Wiley Periodicals, Inc. Article in Journal/Newspaper King Penguins Pygoscelis papua Wiley Online Library Zoo Biology 32 3 257 261 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Traditional methods for sex identification are not applicable to sexually monomorphic species, leading to difficulties in the management of their breeding programs. To identify sex in sexually monomorphic birds, molecular methods have been established. Two established primer pairs (2550F/2718R and p8/p2) amplify the CHD1 gene region from both the Z and W chromosomes. Here, we evaluated the use of these primers for sex identification in four sexually monomorphic penguin species: king penguins ( Aptenodytes patagonicus ), rockhopper penguins ( Eudyptes chrysocome ), gentoo penguins ( Pygoscelis papua ), and Magellanic penguins ( Spheniscus magellanicus ). For all species except rockhopper penguins, primer pair 2550F/2718R resulted in two distinct CHD1Z and CHD1W PCR bands, allowing for sex identification. For rockhopper penguins, only primer pair p8/p2 yielded different CHD1Z and CHD1W bands, which were faint and similar in size making them difficult to distinguish. As a result, we designed a new primer pair (PL/PR) that efficiently determined the gender of individuals from all four penguin species. Sequencing of the PCR products confirmed that they were from the CHD1 gene region. Primer pair PL/PR can be evaluated for use in sexing other penguin species, which will be crucial for the management of new penguin breeding programs. Zoo Biol 32:257–261, 2013. © 2012 Wiley Periodicals, Inc. |
format |
Article in Journal/Newspaper |
author |
Zhang, Peijun Han, Jiabo Liu, Quansheng Zhang, Junxin Zhang, Xianfeng |
spellingShingle |
Zhang, Peijun Han, Jiabo Liu, Quansheng Zhang, Junxin Zhang, Xianfeng Sex Identification of Four Penguin Species Using Locus‐Specific PCR |
author_facet |
Zhang, Peijun Han, Jiabo Liu, Quansheng Zhang, Junxin Zhang, Xianfeng |
author_sort |
Zhang, Peijun |
title |
Sex Identification of Four Penguin Species Using Locus‐Specific PCR |
title_short |
Sex Identification of Four Penguin Species Using Locus‐Specific PCR |
title_full |
Sex Identification of Four Penguin Species Using Locus‐Specific PCR |
title_fullStr |
Sex Identification of Four Penguin Species Using Locus‐Specific PCR |
title_full_unstemmed |
Sex Identification of Four Penguin Species Using Locus‐Specific PCR |
title_sort |
sex identification of four penguin species using locus‐specific pcr |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1002/zoo.21005 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fzoo.21005 https://onlinelibrary.wiley.com/doi/pdf/10.1002/zoo.21005 |
genre |
King Penguins Pygoscelis papua |
genre_facet |
King Penguins Pygoscelis papua |
op_source |
Zoo Biology volume 32, issue 3, page 257-261 ISSN 0733-3188 1098-2361 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/zoo.21005 |
container_title |
Zoo Biology |
container_volume |
32 |
container_issue |
3 |
container_start_page |
257 |
op_container_end_page |
261 |
_version_ |
1800755660878512128 |