Ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, Orcinus orca
Abstract This study aimed to validate the measurements of body temperature and fecal progesterone concentrations as minimally invasive techniques for assessing ovarian cycle in a single sexually mature female killer whale. Rectal temperature data, fecal and blood samples were collected in the dorsal...
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crwiley:10.1002/zoo.20336 2024-06-02T08:09:50+00:00 Ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, Orcinus orca Kusuda, Satoshi Kakizoe, Yuka Kanda, Koji Sengoku, Tomoko Fukumoto, Yohei Adachi, Itsuki Watanabe, Yoko Doi, Osamu 2011 http://dx.doi.org/10.1002/zoo.20336 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fzoo.20336 https://onlinelibrary.wiley.com/doi/pdf/10.1002/zoo.20336 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Zoo Biology volume 30, issue 3, page 285-295 ISSN 0733-3188 1098-2361 journal-article 2011 crwiley https://doi.org/10.1002/zoo.20336 2024-05-03T11:20:42Z Abstract This study aimed to validate the measurements of body temperature and fecal progesterone concentrations as minimally invasive techniques for assessing ovarian cycle in a single sexually mature female killer whale. Rectal temperature data, fecal and blood samples were collected in the dorsal position using routine husbandry training on a voluntary basis. The correlations between rectal temperature and plasma progesterone concentration and between fecal and plasma progesterone concentrations were investigated. Fecal progesterone metabolites were identified by a combination of high‐performance liquid chromatography and enzyme immunoassay. Plasma progesterone concentrations (range: 0.2–18.6 ng/ml) and rectal temperature (range: 35.3–35.9°C) changed cyclically, and cycle lengths were an average (±SD) of 44.9±4.0 days (nine cycles) and 44.6±5.9 days (nine cycles), respectively. Rectal temperature positively correlated with the plasma progesterone concentrations ( r =0.641, P <0.01). There was a visual trend for fecal progesterone profiles to be similar to circulating plasma progesterone profiles. Fecal immunoreactive progestagen analysis resulted in a marked immunoreactive peak of progesterone. The data from the single killer whale indicate that the measurement of rectal temperature is suitable for minimally invasive assessment of the estrous cycle and monitoring the fecal progesterone concentration is useful to assess ovarian luteal activity. Zoo Biol 30:285–295, 2011. © 2010 Wiley‐Liss, Inc. Article in Journal/Newspaper Killer Whale Orca Orcinus orca Killer whale Wiley Online Library Zoo Biology 30 3 285 295 |
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Wiley Online Library |
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English |
description |
Abstract This study aimed to validate the measurements of body temperature and fecal progesterone concentrations as minimally invasive techniques for assessing ovarian cycle in a single sexually mature female killer whale. Rectal temperature data, fecal and blood samples were collected in the dorsal position using routine husbandry training on a voluntary basis. The correlations between rectal temperature and plasma progesterone concentration and between fecal and plasma progesterone concentrations were investigated. Fecal progesterone metabolites were identified by a combination of high‐performance liquid chromatography and enzyme immunoassay. Plasma progesterone concentrations (range: 0.2–18.6 ng/ml) and rectal temperature (range: 35.3–35.9°C) changed cyclically, and cycle lengths were an average (±SD) of 44.9±4.0 days (nine cycles) and 44.6±5.9 days (nine cycles), respectively. Rectal temperature positively correlated with the plasma progesterone concentrations ( r =0.641, P <0.01). There was a visual trend for fecal progesterone profiles to be similar to circulating plasma progesterone profiles. Fecal immunoreactive progestagen analysis resulted in a marked immunoreactive peak of progesterone. The data from the single killer whale indicate that the measurement of rectal temperature is suitable for minimally invasive assessment of the estrous cycle and monitoring the fecal progesterone concentration is useful to assess ovarian luteal activity. Zoo Biol 30:285–295, 2011. © 2010 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Kusuda, Satoshi Kakizoe, Yuka Kanda, Koji Sengoku, Tomoko Fukumoto, Yohei Adachi, Itsuki Watanabe, Yoko Doi, Osamu |
spellingShingle |
Kusuda, Satoshi Kakizoe, Yuka Kanda, Koji Sengoku, Tomoko Fukumoto, Yohei Adachi, Itsuki Watanabe, Yoko Doi, Osamu Ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, Orcinus orca |
author_facet |
Kusuda, Satoshi Kakizoe, Yuka Kanda, Koji Sengoku, Tomoko Fukumoto, Yohei Adachi, Itsuki Watanabe, Yoko Doi, Osamu |
author_sort |
Kusuda, Satoshi |
title |
Ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, Orcinus orca |
title_short |
Ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, Orcinus orca |
title_full |
Ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, Orcinus orca |
title_fullStr |
Ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, Orcinus orca |
title_full_unstemmed |
Ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, Orcinus orca |
title_sort |
ovarian cycle approach by rectal temperature and fecal progesterone in a female killer whale, orcinus orca |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1002/zoo.20336 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fzoo.20336 https://onlinelibrary.wiley.com/doi/pdf/10.1002/zoo.20336 |
genre |
Killer Whale Orca Orcinus orca Killer whale |
genre_facet |
Killer Whale Orca Orcinus orca Killer whale |
op_source |
Zoo Biology volume 30, issue 3, page 285-295 ISSN 0733-3188 1098-2361 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/zoo.20336 |
container_title |
Zoo Biology |
container_volume |
30 |
container_issue |
3 |
container_start_page |
285 |
op_container_end_page |
295 |
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1800755626896261120 |