Prospects for analyzing ancient RNA in preserved materials
An ever increasing wealth of ancient biological material is providing opportunities to study biomolecules. Animal, plant, and microbial samples dating back hundreds, thousands, and even millions of years have been preserved in a dry state under climatic conditions ranging from the arctic to hot dese...
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crwiley:10.1002/wrna.1199 2024-06-02T08:02:24+00:00 Prospects for analyzing ancient RNA in preserved materials Guy, Paul L. University of Otago Brenda Shore Trust 2013 http://dx.doi.org/10.1002/wrna.1199 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fwrna.1199 https://wires.onlinelibrary.wiley.com/doi/pdf/10.1002/wrna.1199 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor WIREs RNA volume 5, issue 1, page 87-94 ISSN 1757-7004 1757-7012 journal-article 2013 crwiley https://doi.org/10.1002/wrna.1199 2024-05-03T11:54:45Z An ever increasing wealth of ancient biological material is providing opportunities to study biomolecules. Animal, plant, and microbial samples dating back hundreds, thousands, and even millions of years have been preserved in a dry state under climatic conditions ranging from the arctic to hot deserts. Various small molecules, often crystalized or polymerized, have improved preservation. Modern methods such as polymerase chain reaction ( PCR ), mass spectrometry, and shotgun sequencing have detected and characterized ancient biomolecules. Modern sequencing has the capacity not only to assemble the whole genome of the target host but also those of the host's parasites, mutualists, and commensals. The study of ancient RNA has barely begun. Several studies show that RNA has been preserved for decades to hundreds of years and the germination of ancient seeds implies that messenger RNA can be preserved for thousands of years. This review briefly examines the types of ancient materials available and assesses their suitability for the study of ancient RNA . Sequencing RNA from this material has the potential not only to illuminate the target host's transcriptome and small RNAs but also to characterize the host's RNA parasites: viruses and viroids. WIREs RNA 2014, 5:87–94. doi: 10.1002/wrna.1199 This article is categorized under: RNA Evolution and Genomics > RNA and Ribonucleoprotein Evolution RNA Methods > RNA Analyses In Vitro and In Silico Article in Journal/Newspaper Arctic Wiley Online Library Arctic WIREs RNA 5 1 87 94 |
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An ever increasing wealth of ancient biological material is providing opportunities to study biomolecules. Animal, plant, and microbial samples dating back hundreds, thousands, and even millions of years have been preserved in a dry state under climatic conditions ranging from the arctic to hot deserts. Various small molecules, often crystalized or polymerized, have improved preservation. Modern methods such as polymerase chain reaction ( PCR ), mass spectrometry, and shotgun sequencing have detected and characterized ancient biomolecules. Modern sequencing has the capacity not only to assemble the whole genome of the target host but also those of the host's parasites, mutualists, and commensals. The study of ancient RNA has barely begun. Several studies show that RNA has been preserved for decades to hundreds of years and the germination of ancient seeds implies that messenger RNA can be preserved for thousands of years. This review briefly examines the types of ancient materials available and assesses their suitability for the study of ancient RNA . Sequencing RNA from this material has the potential not only to illuminate the target host's transcriptome and small RNAs but also to characterize the host's RNA parasites: viruses and viroids. WIREs RNA 2014, 5:87–94. doi: 10.1002/wrna.1199 This article is categorized under: RNA Evolution and Genomics > RNA and Ribonucleoprotein Evolution RNA Methods > RNA Analyses In Vitro and In Silico |
author2 |
University of Otago Brenda Shore Trust |
format |
Article in Journal/Newspaper |
author |
Guy, Paul L. |
spellingShingle |
Guy, Paul L. Prospects for analyzing ancient RNA in preserved materials |
author_facet |
Guy, Paul L. |
author_sort |
Guy, Paul L. |
title |
Prospects for analyzing ancient RNA in preserved materials |
title_short |
Prospects for analyzing ancient RNA in preserved materials |
title_full |
Prospects for analyzing ancient RNA in preserved materials |
title_fullStr |
Prospects for analyzing ancient RNA in preserved materials |
title_full_unstemmed |
Prospects for analyzing ancient RNA in preserved materials |
title_sort |
prospects for analyzing ancient rna in preserved materials |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1002/wrna.1199 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fwrna.1199 https://wires.onlinelibrary.wiley.com/doi/pdf/10.1002/wrna.1199 |
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Arctic |
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Arctic |
genre |
Arctic |
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Arctic |
op_source |
WIREs RNA volume 5, issue 1, page 87-94 ISSN 1757-7004 1757-7012 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/wrna.1199 |
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