Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania
Abstract Coxiella burnetii is an obligate intracellular bacterium that causes Q fever, a zoonotic disease of public health importance. In northern Tanzania, Q fever is a known cause of human febrile illness, but little is known about its distribution in animal hosts. We used a quantitative real‐time...
Published in: | Veterinary Medicine and Science |
---|---|
Main Authors: | , , , , , , , , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2020
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/vms3.401 https://onlinelibrary.wiley.com/doi/pdf/10.1002/vms3.401 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/vms3.401 |
id |
crwiley:10.1002/vms3.401 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/vms3.401 2024-06-02T08:13:44+00:00 Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania Theonest, Ndyetabura O. Carter, Ryan W. Kasagama, Elizabeth Keyyu, Julius D. Shirima, Gabriel M. Tarimo, Rigobert Thomas, Kate M. Wheelhouse, Nick Maro, Venance P. Haydon, Daniel T. Buza, Joram J. Allan, Kathryn J. Halliday, Jo E.B. Biotechnology and Biological Sciences Research Council Wellcome Trust Research Councils UK 2020 http://dx.doi.org/10.1002/vms3.401 https://onlinelibrary.wiley.com/doi/pdf/10.1002/vms3.401 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/vms3.401 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Veterinary Medicine and Science volume 7, issue 3, page 960-967 ISSN 2053-1095 2053-1095 journal-article 2020 crwiley https://doi.org/10.1002/vms3.401 2024-05-03T10:39:51Z Abstract Coxiella burnetii is an obligate intracellular bacterium that causes Q fever, a zoonotic disease of public health importance. In northern Tanzania, Q fever is a known cause of human febrile illness, but little is known about its distribution in animal hosts. We used a quantitative real‐time PCR (qPCR) targeting the insertion element IS1111 to determine the presence and prevalence of C . burnetii infections in small mammals trapped in 12 villages around Moshi Rural and Moshi Urban Districts, northern Tanzania. A total of 382 trapped small mammals of seven species were included in the study; Rattus rattus ( n = 317), Mus musculus ( n = 44), Mastomys natalensis ( n = 8), Acomys wilson ( n = 6), Mus minutoides ( n = 3), Paraxerus flavovottis ( n = 3) and Atelerix albiventris ( n = 1). Overall, 12 (3.1%) of 382 (95% CI: 1.6–5.4) small mammal spleens were positive for C . burnetii DNA. Coxiella burnetii DNA was detected in five of seven of the small mammal species trapped; R . rattus ( n = 7), M . musculus ( n = 1), A . wilson ( n = 2), P . flavovottis ( n = 1) and A. albiventris ( n = 1). Eleven (91.7%) of twelve (95% CI: 61.5–99.8) C . burnetii DNA positive small mammals were trapped within Moshi Urban District. These findings demonstrate that small mammals in Moshi, northern Tanzania are hosts of C . burnetii and may act as a source of C . burnetii infection to humans and other animals. This detection of C . burnetii infections in small mammals should motivate further studies into the contribution of small mammals to the transmission of C . burnetii to humans and animals in this region. Article in Journal/Newspaper Rattus rattus Wiley Online Library Veterinary Medicine and Science 7 3 960 967 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Coxiella burnetii is an obligate intracellular bacterium that causes Q fever, a zoonotic disease of public health importance. In northern Tanzania, Q fever is a known cause of human febrile illness, but little is known about its distribution in animal hosts. We used a quantitative real‐time PCR (qPCR) targeting the insertion element IS1111 to determine the presence and prevalence of C . burnetii infections in small mammals trapped in 12 villages around Moshi Rural and Moshi Urban Districts, northern Tanzania. A total of 382 trapped small mammals of seven species were included in the study; Rattus rattus ( n = 317), Mus musculus ( n = 44), Mastomys natalensis ( n = 8), Acomys wilson ( n = 6), Mus minutoides ( n = 3), Paraxerus flavovottis ( n = 3) and Atelerix albiventris ( n = 1). Overall, 12 (3.1%) of 382 (95% CI: 1.6–5.4) small mammal spleens were positive for C . burnetii DNA. Coxiella burnetii DNA was detected in five of seven of the small mammal species trapped; R . rattus ( n = 7), M . musculus ( n = 1), A . wilson ( n = 2), P . flavovottis ( n = 1) and A. albiventris ( n = 1). Eleven (91.7%) of twelve (95% CI: 61.5–99.8) C . burnetii DNA positive small mammals were trapped within Moshi Urban District. These findings demonstrate that small mammals in Moshi, northern Tanzania are hosts of C . burnetii and may act as a source of C . burnetii infection to humans and other animals. This detection of C . burnetii infections in small mammals should motivate further studies into the contribution of small mammals to the transmission of C . burnetii to humans and animals in this region. |
author2 |
Biotechnology and Biological Sciences Research Council Wellcome Trust Research Councils UK |
format |
Article in Journal/Newspaper |
author |
Theonest, Ndyetabura O. Carter, Ryan W. Kasagama, Elizabeth Keyyu, Julius D. Shirima, Gabriel M. Tarimo, Rigobert Thomas, Kate M. Wheelhouse, Nick Maro, Venance P. Haydon, Daniel T. Buza, Joram J. Allan, Kathryn J. Halliday, Jo E.B. |
spellingShingle |
Theonest, Ndyetabura O. Carter, Ryan W. Kasagama, Elizabeth Keyyu, Julius D. Shirima, Gabriel M. Tarimo, Rigobert Thomas, Kate M. Wheelhouse, Nick Maro, Venance P. Haydon, Daniel T. Buza, Joram J. Allan, Kathryn J. Halliday, Jo E.B. Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania |
author_facet |
Theonest, Ndyetabura O. Carter, Ryan W. Kasagama, Elizabeth Keyyu, Julius D. Shirima, Gabriel M. Tarimo, Rigobert Thomas, Kate M. Wheelhouse, Nick Maro, Venance P. Haydon, Daniel T. Buza, Joram J. Allan, Kathryn J. Halliday, Jo E.B. |
author_sort |
Theonest, Ndyetabura O. |
title |
Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania |
title_short |
Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania |
title_full |
Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania |
title_fullStr |
Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania |
title_full_unstemmed |
Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania |
title_sort |
molecular detection of coxiella burnetii infection in small mammals from moshi rural and urban districts, northern tanzania |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1002/vms3.401 https://onlinelibrary.wiley.com/doi/pdf/10.1002/vms3.401 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/vms3.401 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Veterinary Medicine and Science volume 7, issue 3, page 960-967 ISSN 2053-1095 2053-1095 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/vms3.401 |
container_title |
Veterinary Medicine and Science |
container_volume |
7 |
container_issue |
3 |
container_start_page |
960 |
op_container_end_page |
967 |
_version_ |
1800737329641422848 |