Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa
Abstract Background Coxiella burnetii is a bacterial pathogen that causes query fever and coxiellosis in humans and animals, respectively. There is a scarcity of studies on the prevalence of C. burnetii infections in rats and chickens in South Africa. Objective The aim of this study was to determine...
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crwiley:10.1002/vms3.1192 2024-06-02T08:13:43+00:00 Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa Ramatla, Tsepo Khumalo, Zamantungwa T. H. Matshotshi, Asiashu Lekota, Kgaugelo E. Taioe, Moeti O. Thekisoe, Oriel 2023 http://dx.doi.org/10.1002/vms3.1192 https://onlinelibrary.wiley.com/doi/pdf/10.1002/vms3.1192 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Veterinary Medicine and Science volume 9, issue 5, page 2185-2191 ISSN 2053-1095 2053-1095 journal-article 2023 crwiley https://doi.org/10.1002/vms3.1192 2024-05-03T11:46:17Z Abstract Background Coxiella burnetii is a bacterial pathogen that causes query fever and coxiellosis in humans and animals, respectively. There is a scarcity of studies on the prevalence of C. burnetii infections in rats and chickens in South Africa. Objective The aim of this study was to determine the occurrence of C. burnetii in rats and chickens sampled from poultry farms in the North West Province of South Africa. Methods DNA was extracted from rodent kidneys ( n = 68) and chicken faeces ( n = 52). Two rodent pest species, namely Rattus rattus and Rattus tanezumi , were identified by analysis of CO1 gene sequences. Detection of C. burnetii was carried out using polymerase chain reaction assays targeting 23S rRNA , 16S rRNA and IS111 markers. Results C. burnetii was detected in 16.2%, 8.8% and 25% of R. rattus , R. tanezumi and chickens, respectively. Conclusions The findings in this study demonstrate that rodents and chickens are harbouring C. burnetii at sampled poultry farms. There should be frequent screening for C. burnetii in poultry operations. The likelihood of future transmission between rodents and chickens, including humans, also needs to be investigated. Article in Journal/Newspaper Rattus rattus Wiley Online Library Veterinary Medicine and Science 9 5 2185 2191 |
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Abstract Background Coxiella burnetii is a bacterial pathogen that causes query fever and coxiellosis in humans and animals, respectively. There is a scarcity of studies on the prevalence of C. burnetii infections in rats and chickens in South Africa. Objective The aim of this study was to determine the occurrence of C. burnetii in rats and chickens sampled from poultry farms in the North West Province of South Africa. Methods DNA was extracted from rodent kidneys ( n = 68) and chicken faeces ( n = 52). Two rodent pest species, namely Rattus rattus and Rattus tanezumi , were identified by analysis of CO1 gene sequences. Detection of C. burnetii was carried out using polymerase chain reaction assays targeting 23S rRNA , 16S rRNA and IS111 markers. Results C. burnetii was detected in 16.2%, 8.8% and 25% of R. rattus , R. tanezumi and chickens, respectively. Conclusions The findings in this study demonstrate that rodents and chickens are harbouring C. burnetii at sampled poultry farms. There should be frequent screening for C. burnetii in poultry operations. The likelihood of future transmission between rodents and chickens, including humans, also needs to be investigated. |
format |
Article in Journal/Newspaper |
author |
Ramatla, Tsepo Khumalo, Zamantungwa T. H. Matshotshi, Asiashu Lekota, Kgaugelo E. Taioe, Moeti O. Thekisoe, Oriel |
spellingShingle |
Ramatla, Tsepo Khumalo, Zamantungwa T. H. Matshotshi, Asiashu Lekota, Kgaugelo E. Taioe, Moeti O. Thekisoe, Oriel Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa |
author_facet |
Ramatla, Tsepo Khumalo, Zamantungwa T. H. Matshotshi, Asiashu Lekota, Kgaugelo E. Taioe, Moeti O. Thekisoe, Oriel |
author_sort |
Ramatla, Tsepo |
title |
Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa |
title_short |
Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa |
title_full |
Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa |
title_fullStr |
Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa |
title_full_unstemmed |
Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa |
title_sort |
molecular detection of coxiella burnetii and coxiella species in rats and chickens from poultry farms in north west province, south africa |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/vms3.1192 https://onlinelibrary.wiley.com/doi/pdf/10.1002/vms3.1192 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Veterinary Medicine and Science volume 9, issue 5, page 2185-2191 ISSN 2053-1095 2053-1095 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/vms3.1192 |
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Veterinary Medicine and Science |
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9 |
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5 |
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2185 |
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2191 |
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1800737318598868992 |