A microscale test to measure petroleum oil toxicity to mummichog embryos
Abstract A test was developed to compare the toxicity of different petroleum oils to mummichog ( Fundulus heteroclitus ) embryos. Fertilized eggs were incubated for 11 days at 22.5°C directly on the surface of oil‐contaminated sand without a superficial water layer. The mortality rates, the stage of...
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crwiley:10.1002/tox.10049 2024-04-28T07:53:28+00:00 A microscale test to measure petroleum oil toxicity to mummichog embryos Couillard, C. M. 2002 http://dx.doi.org/10.1002/tox.10049 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Ftox.10049 https://onlinelibrary.wiley.com/doi/pdf/10.1002/tox.10049 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Toxicology volume 17, issue 3, page 195-202 ISSN 1520-4081 1522-7278 Health, Toxicology and Mutagenesis Management, Monitoring, Policy and Law Toxicology General Medicine journal-article 2002 crwiley https://doi.org/10.1002/tox.10049 2024-04-02T08:47:19Z Abstract A test was developed to compare the toxicity of different petroleum oils to mummichog ( Fundulus heteroclitus ) embryos. Fertilized eggs were incubated for 11 days at 22.5°C directly on the surface of oil‐contaminated sand without a superficial water layer. The mortality rates, the stage of development, and the prevalence of malformations were determined. No effect was found in controls incubated on sand with water and mineral oil as compared with controls on sand with water alone. Two weathered oils, an Alaska North Slope crude oil (ANCO) and a Mesa light crude oil (MLCO), produced similar symptoms of toxicity: retarded growth and development, pericardial edema, hemostasis, hemorrhages, and spinal deformities. These symptoms are consistent with those observed in other fish species exposed to petroleum oils, suggesting that the results of the bioassay would be applicable to other species. MLCO was more embryotoxic than ANCO. The minimal oil concentrations causing a significant reduction in body length were 4.5 μg oil/g sand for MLCO and 12.7 μg oil/g for ANCO, indicating the assay is sensitive. The slopes and the intercepts of the relationships between concentration and growth did not differ in three dose–response experiments conducted with each oil, indicating that the assay is reliable. Finally, the bioassay is less costly than other available options to assess the toxicity of petroleum oils to marine fish embryos. Further work to improve the standardization of the assay will involve comparison of the toxicity of petroleum oils with reference toxicants and selection of a standard substrate. © 2002 Wiley Periodicals, Inc. Environ Toxicol 17: 195–202, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/tox.10049 Article in Journal/Newspaper Alaska North Slope north slope Alaska Wiley Online Library Environmental Toxicology 17 3 195 202 |
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Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Health, Toxicology and Mutagenesis Management, Monitoring, Policy and Law Toxicology General Medicine |
spellingShingle |
Health, Toxicology and Mutagenesis Management, Monitoring, Policy and Law Toxicology General Medicine Couillard, C. M. A microscale test to measure petroleum oil toxicity to mummichog embryos |
topic_facet |
Health, Toxicology and Mutagenesis Management, Monitoring, Policy and Law Toxicology General Medicine |
description |
Abstract A test was developed to compare the toxicity of different petroleum oils to mummichog ( Fundulus heteroclitus ) embryos. Fertilized eggs were incubated for 11 days at 22.5°C directly on the surface of oil‐contaminated sand without a superficial water layer. The mortality rates, the stage of development, and the prevalence of malformations were determined. No effect was found in controls incubated on sand with water and mineral oil as compared with controls on sand with water alone. Two weathered oils, an Alaska North Slope crude oil (ANCO) and a Mesa light crude oil (MLCO), produced similar symptoms of toxicity: retarded growth and development, pericardial edema, hemostasis, hemorrhages, and spinal deformities. These symptoms are consistent with those observed in other fish species exposed to petroleum oils, suggesting that the results of the bioassay would be applicable to other species. MLCO was more embryotoxic than ANCO. The minimal oil concentrations causing a significant reduction in body length were 4.5 μg oil/g sand for MLCO and 12.7 μg oil/g for ANCO, indicating the assay is sensitive. The slopes and the intercepts of the relationships between concentration and growth did not differ in three dose–response experiments conducted with each oil, indicating that the assay is reliable. Finally, the bioassay is less costly than other available options to assess the toxicity of petroleum oils to marine fish embryos. Further work to improve the standardization of the assay will involve comparison of the toxicity of petroleum oils with reference toxicants and selection of a standard substrate. © 2002 Wiley Periodicals, Inc. Environ Toxicol 17: 195–202, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/tox.10049 |
format |
Article in Journal/Newspaper |
author |
Couillard, C. M. |
author_facet |
Couillard, C. M. |
author_sort |
Couillard, C. M. |
title |
A microscale test to measure petroleum oil toxicity to mummichog embryos |
title_short |
A microscale test to measure petroleum oil toxicity to mummichog embryos |
title_full |
A microscale test to measure petroleum oil toxicity to mummichog embryos |
title_fullStr |
A microscale test to measure petroleum oil toxicity to mummichog embryos |
title_full_unstemmed |
A microscale test to measure petroleum oil toxicity to mummichog embryos |
title_sort |
microscale test to measure petroleum oil toxicity to mummichog embryos |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1002/tox.10049 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Ftox.10049 https://onlinelibrary.wiley.com/doi/pdf/10.1002/tox.10049 |
genre |
Alaska North Slope north slope Alaska |
genre_facet |
Alaska North Slope north slope Alaska |
op_source |
Environmental Toxicology volume 17, issue 3, page 195-202 ISSN 1520-4081 1522-7278 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/tox.10049 |
container_title |
Environmental Toxicology |
container_volume |
17 |
container_issue |
3 |
container_start_page |
195 |
op_container_end_page |
202 |
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1797572024851234816 |