A novel saccharifying α‐amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization
A DNA segment encoding a novel α‐amylase was cloned from the Antarctic psychrotolerant fungi Geomyces pannorum . The gene has an open reading frame of 1761 bp, deduced 586 amino acids, revealing a relatively low homology to α‐amylases from diverse fungal genera. The α‐amylase gene was overexpressed...
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crwiley:10.1002/star.201500077 2024-06-02T07:57:09+00:00 A novel saccharifying α‐amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization Gao, Bei Mao, Youzhi Zhang, Lujia He, Lei Wei, Dongzhi National Natural Foundation of China Fundamental Research Funds for the Central Universities National High Technology Research and Development Program of China 2015 http://dx.doi.org/10.1002/star.201500077 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fstar.201500077 https://onlinelibrary.wiley.com/doi/pdf/10.1002/star.201500077 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Starch - Stärke volume 68, issue 1-2, page 20-28 ISSN 0038-9056 1521-379X journal-article 2015 crwiley https://doi.org/10.1002/star.201500077 2024-05-03T11:33:58Z A DNA segment encoding a novel α‐amylase was cloned from the Antarctic psychrotolerant fungi Geomyces pannorum . The gene has an open reading frame of 1761 bp, deduced 586 amino acids, revealing a relatively low homology to α‐amylases from diverse fungal genera. The α‐amylase gene was overexpressed in Pichia pastoris , and the recombinant α‐amylase was purified and characterized. Enzymatic activity was optimal at pH 6.0 and 70°C, and the specific activity was 9.78 × 10 3 U/mg when using soluble starch as substrate. Moreover, the enzyme retained more than 90% of its maximum activity over a wide pH range (6.0–9.0). Exceptionally, the main hydrolysis products of soluble starch catalyzed by the enzyme were glucose and maltose, of which glucose reached a concentration of 68.8% (w/w). This is the first report on identifying thermophilic fungal amylase from psychrotolerant fungi. Also, the preliminary study on the high‐glucose‐producing α‐amylase with detailed enzymatic properties shows a potential application prospect in syrup industry. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic Starch - Stärke 68 1-2 20 28 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
A DNA segment encoding a novel α‐amylase was cloned from the Antarctic psychrotolerant fungi Geomyces pannorum . The gene has an open reading frame of 1761 bp, deduced 586 amino acids, revealing a relatively low homology to α‐amylases from diverse fungal genera. The α‐amylase gene was overexpressed in Pichia pastoris , and the recombinant α‐amylase was purified and characterized. Enzymatic activity was optimal at pH 6.0 and 70°C, and the specific activity was 9.78 × 10 3 U/mg when using soluble starch as substrate. Moreover, the enzyme retained more than 90% of its maximum activity over a wide pH range (6.0–9.0). Exceptionally, the main hydrolysis products of soluble starch catalyzed by the enzyme were glucose and maltose, of which glucose reached a concentration of 68.8% (w/w). This is the first report on identifying thermophilic fungal amylase from psychrotolerant fungi. Also, the preliminary study on the high‐glucose‐producing α‐amylase with detailed enzymatic properties shows a potential application prospect in syrup industry. |
author2 |
National Natural Foundation of China Fundamental Research Funds for the Central Universities National High Technology Research and Development Program of China |
format |
Article in Journal/Newspaper |
author |
Gao, Bei Mao, Youzhi Zhang, Lujia He, Lei Wei, Dongzhi |
spellingShingle |
Gao, Bei Mao, Youzhi Zhang, Lujia He, Lei Wei, Dongzhi A novel saccharifying α‐amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization |
author_facet |
Gao, Bei Mao, Youzhi Zhang, Lujia He, Lei Wei, Dongzhi |
author_sort |
Gao, Bei |
title |
A novel saccharifying α‐amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization |
title_short |
A novel saccharifying α‐amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization |
title_full |
A novel saccharifying α‐amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization |
title_fullStr |
A novel saccharifying α‐amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization |
title_full_unstemmed |
A novel saccharifying α‐amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization |
title_sort |
novel saccharifying α‐amylase of antarctic psychrotolerant fungi geomyces pannorum: gene cloning, functional expression, and characterization |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1002/star.201500077 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fstar.201500077 https://onlinelibrary.wiley.com/doi/pdf/10.1002/star.201500077 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Starch - Stärke volume 68, issue 1-2, page 20-28 ISSN 0038-9056 1521-379X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/star.201500077 |
container_title |
Starch - Stärke |
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68 |
container_issue |
1-2 |
container_start_page |
20 |
op_container_end_page |
28 |
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1800739242231463936 |