Gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated C 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: A comparative study
Rationale The efficiency of selected ion monitoring (SIM) and selected reaction monitoring (SRM) analyses for the quantification of three mono‐, di‐ and tri‐unsaturated highly branched isoprenoid (HBI) alkenes (IP 25 , IPSO 25 and HBI III, respectively), often used as proxies for the occurrence of A...
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crwiley:10.1002/rcm.9704 2024-06-02T07:58:16+00:00 Gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated C 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: A comparative study Rontani, Jean‐François Smik, Lukas Divine, Dmitry Husum, Katrine Belt, Simon T. European Regional Development Fund 2024 http://dx.doi.org/10.1002/rcm.9704 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Rapid Communications in Mass Spectrometry volume 38, issue 6 ISSN 0951-4198 1097-0231 journal-article 2024 crwiley https://doi.org/10.1002/rcm.9704 2024-05-03T10:41:56Z Rationale The efficiency of selected ion monitoring (SIM) and selected reaction monitoring (SRM) analyses for the quantification of three mono‐, di‐ and tri‐unsaturated highly branched isoprenoid (HBI) alkenes (IP 25 , IPSO 25 and HBI III, respectively), often used as proxies for the occurrence of Arctic and Antarctic sea ice or the adjacent open waters, was compared. Methods Gas chromatography (GC)–mass spectrometry (MS)/SIM and GC/MS/MS/SRM analyses were carried out on dilute solutions made from purified standards of these three HBIs, and then on hydrocarbon fractions of several sediment and sea ice sample extracts. More efficient and specific SRM transitions were selected after collision‐induced dissociation of each precursor ion at different collision energies. Results SRM analysis avoided any overestimation of IP 25 resulting from the contribution of the coeluting 13 C mass isotopomer of IPSO 25 (M + ˙ + 2) to the SIM target ion. In contrast, SRM analysis is less reliable for IPSO 25 quantification in cases where several regio‐isomers are present, likely due to intense double bond migrations following electron impact. In the case of HBI III, SRM analysis constitutes a potentially suitable alternative to SIM analysis, especially in terms of improving limit of detection. Conclusions Despite the intense migrations of HBI double bonds under electron ionization, the selected SRM transitions should be more suitable than SIM target ions for IP 25 and HBI III quantification in complex hydrocarbon fractions of natural samples. However, the advantage is less evident for IPSO 25 due to the presence of numerous regio‐isomers. Article in Journal/Newspaper Antarc* Antarctic Arctic Sea ice Wiley Online Library Antarctic Arctic Rapid Communications in Mass Spectrometry 38 6 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Rationale The efficiency of selected ion monitoring (SIM) and selected reaction monitoring (SRM) analyses for the quantification of three mono‐, di‐ and tri‐unsaturated highly branched isoprenoid (HBI) alkenes (IP 25 , IPSO 25 and HBI III, respectively), often used as proxies for the occurrence of Arctic and Antarctic sea ice or the adjacent open waters, was compared. Methods Gas chromatography (GC)–mass spectrometry (MS)/SIM and GC/MS/MS/SRM analyses were carried out on dilute solutions made from purified standards of these three HBIs, and then on hydrocarbon fractions of several sediment and sea ice sample extracts. More efficient and specific SRM transitions were selected after collision‐induced dissociation of each precursor ion at different collision energies. Results SRM analysis avoided any overestimation of IP 25 resulting from the contribution of the coeluting 13 C mass isotopomer of IPSO 25 (M + ˙ + 2) to the SIM target ion. In contrast, SRM analysis is less reliable for IPSO 25 quantification in cases where several regio‐isomers are present, likely due to intense double bond migrations following electron impact. In the case of HBI III, SRM analysis constitutes a potentially suitable alternative to SIM analysis, especially in terms of improving limit of detection. Conclusions Despite the intense migrations of HBI double bonds under electron ionization, the selected SRM transitions should be more suitable than SIM target ions for IP 25 and HBI III quantification in complex hydrocarbon fractions of natural samples. However, the advantage is less evident for IPSO 25 due to the presence of numerous regio‐isomers. |
author2 |
European Regional Development Fund |
format |
Article in Journal/Newspaper |
author |
Rontani, Jean‐François Smik, Lukas Divine, Dmitry Husum, Katrine Belt, Simon T. |
spellingShingle |
Rontani, Jean‐François Smik, Lukas Divine, Dmitry Husum, Katrine Belt, Simon T. Gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated C 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: A comparative study |
author_facet |
Rontani, Jean‐François Smik, Lukas Divine, Dmitry Husum, Katrine Belt, Simon T. |
author_sort |
Rontani, Jean‐François |
title |
Gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated C 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: A comparative study |
title_short |
Gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated C 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: A comparative study |
title_full |
Gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated C 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: A comparative study |
title_fullStr |
Gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated C 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: A comparative study |
title_full_unstemmed |
Gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated C 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: A comparative study |
title_sort |
gas chromatography–mass spectrometry selected ion monitoring and gas chromatography–tandem mass spectrometry selected reaction monitoring analyses of mono‐, di‐ and tri‐unsaturated c 25 highly branched isoprenoid alkene biomarkers in sea ice and sediment samples: a comparative study |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1002/rcm.9704 |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic Sea ice |
genre_facet |
Antarc* Antarctic Arctic Sea ice |
op_source |
Rapid Communications in Mass Spectrometry volume 38, issue 6 ISSN 0951-4198 1097-0231 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/rcm.9704 |
container_title |
Rapid Communications in Mass Spectrometry |
container_volume |
38 |
container_issue |
6 |
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1800741551552331776 |