Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods?
Rationale Stable isotope analysis (SIA) is a powerful tool to estimate dietary links between polar zooplankton. However, the presence of highly variable 12 C‐rich lipids may skew estimations as they are depleted in 13 C relative to proteins and carbohydrates, consequently masking carbon signals from...
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crwiley:10.1002/rcm.8384 2024-06-02T07:58:38+00:00 Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? Weldrick, Christine K. Trebilco, Rowan Swadling, Kerrie M. Australian Antarctic Division Australian Nuclear Science and Technology Organisation Ecological Society of Australia Holsworth Wildlife Research Endowment 2019 http://dx.doi.org/10.1002/rcm.8384 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.8384 http://onlinelibrary.wiley.com/wol1/doi/10.1002/rcm.8384/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Rapid Communications in Mass Spectrometry volume 33, issue 6, page 569-578 ISSN 0951-4198 1097-0231 journal-article 2019 crwiley https://doi.org/10.1002/rcm.8384 2024-05-03T10:57:03Z Rationale Stable isotope analysis (SIA) is a powerful tool to estimate dietary links between polar zooplankton. However, the presence of highly variable 12 C‐rich lipids may skew estimations as they are depleted in 13 C relative to proteins and carbohydrates, consequently masking carbon signals from food sources. Lipid effects on pteropod‐specific values requires examining, since accounting for lipids is rarely conducted among the few existing pteropod‐related SIA studies. It is currently unclear whether lipid correction is necessary prior to SIA of pteropods. Methods Whole bodies of three species of pteropods ( Clio pyramidata f. sulcata , Clione limacina antarctica , and Spongiobranchaea australis ) sampled from the Southern Ocean were lipid‐extracted chemically to test the effects on δ 13 C and δ 15 N values ( n = 38 individuals in total). We determined the average change in δ 13 C values for each treatment, and compared this offset with those from published normalization models. We tested lipid correction effects on isotopic niche dispersion metrics to compare interpretations surrounding food web dynamics. Results Pteropods with lipids removed had δ 13 C values up to 4.5‰ higher than bulk samples. However, lipid extraction also produced higher δ 15 N values than bulk samples. Isotopic niche overlaps between untreated pteropods and their potential food sources were significantly different from overlaps generated between lipid‐corrected pteropods and their potential food sources. Data converted using several published normalization models did not reveal significant differences among various calculated niche metrics, including standard ellipse and total area. Conclusions We recommend accounting for lipids via chemical extraction or mathematical normalization before applying SIA to calculate ecological niche metrics, particularly for organisms with moderate to high lipid content such as polar pteropods. Failure to account for lipids may result in misinterpretations of niche dimensions and overlap and, ... Article in Journal/Newspaper Antarc* Antarctica Clione limacina Limacina antarctica Southern Ocean Wiley Online Library Southern Ocean Rapid Communications in Mass Spectrometry 33 6 569 578 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Rationale Stable isotope analysis (SIA) is a powerful tool to estimate dietary links between polar zooplankton. However, the presence of highly variable 12 C‐rich lipids may skew estimations as they are depleted in 13 C relative to proteins and carbohydrates, consequently masking carbon signals from food sources. Lipid effects on pteropod‐specific values requires examining, since accounting for lipids is rarely conducted among the few existing pteropod‐related SIA studies. It is currently unclear whether lipid correction is necessary prior to SIA of pteropods. Methods Whole bodies of three species of pteropods ( Clio pyramidata f. sulcata , Clione limacina antarctica , and Spongiobranchaea australis ) sampled from the Southern Ocean were lipid‐extracted chemically to test the effects on δ 13 C and δ 15 N values ( n = 38 individuals in total). We determined the average change in δ 13 C values for each treatment, and compared this offset with those from published normalization models. We tested lipid correction effects on isotopic niche dispersion metrics to compare interpretations surrounding food web dynamics. Results Pteropods with lipids removed had δ 13 C values up to 4.5‰ higher than bulk samples. However, lipid extraction also produced higher δ 15 N values than bulk samples. Isotopic niche overlaps between untreated pteropods and their potential food sources were significantly different from overlaps generated between lipid‐corrected pteropods and their potential food sources. Data converted using several published normalization models did not reveal significant differences among various calculated niche metrics, including standard ellipse and total area. Conclusions We recommend accounting for lipids via chemical extraction or mathematical normalization before applying SIA to calculate ecological niche metrics, particularly for organisms with moderate to high lipid content such as polar pteropods. Failure to account for lipids may result in misinterpretations of niche dimensions and overlap and, ... |
author2 |
Australian Antarctic Division Australian Nuclear Science and Technology Organisation Ecological Society of Australia Holsworth Wildlife Research Endowment |
format |
Article in Journal/Newspaper |
author |
Weldrick, Christine K. Trebilco, Rowan Swadling, Kerrie M. |
spellingShingle |
Weldrick, Christine K. Trebilco, Rowan Swadling, Kerrie M. Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
author_facet |
Weldrick, Christine K. Trebilco, Rowan Swadling, Kerrie M. |
author_sort |
Weldrick, Christine K. |
title |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_short |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_full |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_fullStr |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_full_unstemmed |
Can lipid removal affect interpretation of resource partitioning from stable isotopes in Southern Ocean pteropods? |
title_sort |
can lipid removal affect interpretation of resource partitioning from stable isotopes in southern ocean pteropods? |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1002/rcm.8384 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.8384 http://onlinelibrary.wiley.com/wol1/doi/10.1002/rcm.8384/fullpdf |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Antarc* Antarctica Clione limacina Limacina antarctica Southern Ocean |
genre_facet |
Antarc* Antarctica Clione limacina Limacina antarctica Southern Ocean |
op_source |
Rapid Communications in Mass Spectrometry volume 33, issue 6, page 569-578 ISSN 0951-4198 1097-0231 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/rcm.8384 |
container_title |
Rapid Communications in Mass Spectrometry |
container_volume |
33 |
container_issue |
6 |
container_start_page |
569 |
op_container_end_page |
578 |
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1800742055401488384 |