Evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios
Rationale Stable isotope analysis integrates diet information over a time period specific to the type of tissue sampled. For metabolically active skin of free‐ranging cetaceans, cells are generated at the basal layer of the skin and migrate outward until they eventually slough off, suggesting potent...
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crwiley:10.1002/rcm.8168 2024-09-15T18:37:32+00:00 Evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios Wild, Lauren A. Chenoweth, Ellen M. Mueter, Franz J. Straley, Janice M. National Institute of General Medical Sciences 2018 http://dx.doi.org/10.1002/rcm.8168 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.8168 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.8168 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Rapid Communications in Mass Spectrometry volume 32, issue 16, page 1425-1438 ISSN 0951-4198 1097-0231 journal-article 2018 crwiley https://doi.org/10.1002/rcm.8168 2024-08-09T04:21:58Z Rationale Stable isotope analysis integrates diet information over a time period specific to the type of tissue sampled. For metabolically active skin of free‐ranging cetaceans, cells are generated at the basal layer of the skin and migrate outward until they eventually slough off, suggesting potential for a dietary time series. Methods Skin samples from cetaceans were analyzed using continuous‐flow elemental analyzer isotope ratio mass spectrometry. We used ANOVAs to compare the variability of δ 13 C and δ 15 N values within and among layers and columns (“cores”) of the skin of a fin, humpback, and sperm whale. We then used mixed‐effects models to analyze isotopic variability among layers of 28 sperm whale skin samples, over the course of a season and among years. Results We found layer to be a significant predictor of δ 13 C values in the sperm whale's skin, and δ 15 N values in the humpback whale's skin. There was no evidence for significant differences in δ 15 N or δ 13 C values among cores for any species. Mixed‐effects models selected layer and day of the year as significant predictors of δ 13 C and δ 15 N values in sperm whale skin across individuals sampled during the summer months in the Gulf of Alaska. Conclusions These results suggest that skin samples from cetaceans may be subsampled to reflect diet during a narrower time period; specifically different layers of skin may contain a dietary time series. This underscores the importance of selecting an appropriate portion of skin to analyze based on the species and objectives of the study. Article in Journal/Newspaper Sperm whale Alaska Wiley Online Library Rapid Communications in Mass Spectrometry 32 16 1425 1438 |
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English |
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Rationale Stable isotope analysis integrates diet information over a time period specific to the type of tissue sampled. For metabolically active skin of free‐ranging cetaceans, cells are generated at the basal layer of the skin and migrate outward until they eventually slough off, suggesting potential for a dietary time series. Methods Skin samples from cetaceans were analyzed using continuous‐flow elemental analyzer isotope ratio mass spectrometry. We used ANOVAs to compare the variability of δ 13 C and δ 15 N values within and among layers and columns (“cores”) of the skin of a fin, humpback, and sperm whale. We then used mixed‐effects models to analyze isotopic variability among layers of 28 sperm whale skin samples, over the course of a season and among years. Results We found layer to be a significant predictor of δ 13 C values in the sperm whale's skin, and δ 15 N values in the humpback whale's skin. There was no evidence for significant differences in δ 15 N or δ 13 C values among cores for any species. Mixed‐effects models selected layer and day of the year as significant predictors of δ 13 C and δ 15 N values in sperm whale skin across individuals sampled during the summer months in the Gulf of Alaska. Conclusions These results suggest that skin samples from cetaceans may be subsampled to reflect diet during a narrower time period; specifically different layers of skin may contain a dietary time series. This underscores the importance of selecting an appropriate portion of skin to analyze based on the species and objectives of the study. |
author2 |
National Institute of General Medical Sciences |
format |
Article in Journal/Newspaper |
author |
Wild, Lauren A. Chenoweth, Ellen M. Mueter, Franz J. Straley, Janice M. |
spellingShingle |
Wild, Lauren A. Chenoweth, Ellen M. Mueter, Franz J. Straley, Janice M. Evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios |
author_facet |
Wild, Lauren A. Chenoweth, Ellen M. Mueter, Franz J. Straley, Janice M. |
author_sort |
Wild, Lauren A. |
title |
Evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios |
title_short |
Evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios |
title_full |
Evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios |
title_fullStr |
Evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios |
title_full_unstemmed |
Evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios |
title_sort |
evidence for dietary time series in layers of cetacean skin using stable carbon and nitrogen isotope ratios |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/rcm.8168 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.8168 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.8168 |
genre |
Sperm whale Alaska |
genre_facet |
Sperm whale Alaska |
op_source |
Rapid Communications in Mass Spectrometry volume 32, issue 16, page 1425-1438 ISSN 0951-4198 1097-0231 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/rcm.8168 |
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Rapid Communications in Mass Spectrometry |
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32 |
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16 |
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1425 |
op_container_end_page |
1438 |
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1810481910249422848 |