Carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( Cystophora cristata): Application for diet analysis
Rationale Intrinsic biogeochemical markers, such as stable isotope ratios of carbon, nitrogen and sulphur, are increasingly used to trace the trophic ecology of marine top predators. However, insufficient knowledge of fractionation processes in tissues continues to hamper the use of these markers. M...
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crwiley:10.1002/rcm.7955 2024-06-23T07:52:20+00:00 Carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( Cystophora cristata): Application for diet analysis Pinzone, Marianna Acquarone, Mario Huyghebaert, Loreen Sturaro, Nicolas Michel, Loïc N. Siebert, Ursula Das, Krishna Fonds pour la Formation à la Recherche dans l’Industrie et dans l’Agriculture 2017 http://dx.doi.org/10.1002/rcm.7955 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.7955 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.7955 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Rapid Communications in Mass Spectrometry volume 31, issue 20, page 1720-1728 ISSN 0951-4198 1097-0231 journal-article 2017 crwiley https://doi.org/10.1002/rcm.7955 2024-06-13T04:24:09Z Rationale Intrinsic biogeochemical markers, such as stable isotope ratios of carbon, nitrogen and sulphur, are increasingly used to trace the trophic ecology of marine top predators. However, insufficient knowledge of fractionation processes in tissues continues to hamper the use of these markers. Methods We performed a controlled feeding experiment with eight juvenile hooded seals ( Cystophora cristata ) that were held on a herring‐based diet ( Clupea harengus ) for two years. Stable isotope ratios were measured via isotope ratio mass spectrometry in three of their tissues and related to values of these markers in their diet. Results Diet‐tissue isotope enrichment (trophic enrichment factor, TEF) values between dietary herring and seal tissues for carbon (Δ 13 C) were +0.7 ‰ for red blood cells, +1.9 ‰ for hair and +1.1 ‰ for muscle. The TEFs for nitrogen trophic (Δ 15 N) were +3.3 ‰ for red blood cells, +3.6 ‰ for hair and +4.3 ‰ for muscle. For sulphur, the Δ 34 S values were +1.1 ‰ for red blood cells, +1.0 ‰ for hair and +0.9 ‰ for muscle. Conclusions These enrichment values were greater than those previously measured in adult seals. This increase may be related to the higher rate of protein synthesis and catabolism in growing animals. This study is the first report on sulphur isotope enrichment values for a marine mammal species. Article in Journal/Newspaper Cystophora cristata hooded seal Wiley Online Library Rapid Communications in Mass Spectrometry 31 20 1720 1728 |
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Wiley Online Library |
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English |
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Rationale Intrinsic biogeochemical markers, such as stable isotope ratios of carbon, nitrogen and sulphur, are increasingly used to trace the trophic ecology of marine top predators. However, insufficient knowledge of fractionation processes in tissues continues to hamper the use of these markers. Methods We performed a controlled feeding experiment with eight juvenile hooded seals ( Cystophora cristata ) that were held on a herring‐based diet ( Clupea harengus ) for two years. Stable isotope ratios were measured via isotope ratio mass spectrometry in three of their tissues and related to values of these markers in their diet. Results Diet‐tissue isotope enrichment (trophic enrichment factor, TEF) values between dietary herring and seal tissues for carbon (Δ 13 C) were +0.7 ‰ for red blood cells, +1.9 ‰ for hair and +1.1 ‰ for muscle. The TEFs for nitrogen trophic (Δ 15 N) were +3.3 ‰ for red blood cells, +3.6 ‰ for hair and +4.3 ‰ for muscle. For sulphur, the Δ 34 S values were +1.1 ‰ for red blood cells, +1.0 ‰ for hair and +0.9 ‰ for muscle. Conclusions These enrichment values were greater than those previously measured in adult seals. This increase may be related to the higher rate of protein synthesis and catabolism in growing animals. This study is the first report on sulphur isotope enrichment values for a marine mammal species. |
author2 |
Fonds pour la Formation à la Recherche dans l’Industrie et dans l’Agriculture |
format |
Article in Journal/Newspaper |
author |
Pinzone, Marianna Acquarone, Mario Huyghebaert, Loreen Sturaro, Nicolas Michel, Loïc N. Siebert, Ursula Das, Krishna |
spellingShingle |
Pinzone, Marianna Acquarone, Mario Huyghebaert, Loreen Sturaro, Nicolas Michel, Loïc N. Siebert, Ursula Das, Krishna Carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( Cystophora cristata): Application for diet analysis |
author_facet |
Pinzone, Marianna Acquarone, Mario Huyghebaert, Loreen Sturaro, Nicolas Michel, Loïc N. Siebert, Ursula Das, Krishna |
author_sort |
Pinzone, Marianna |
title |
Carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( Cystophora cristata): Application for diet analysis |
title_short |
Carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( Cystophora cristata): Application for diet analysis |
title_full |
Carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( Cystophora cristata): Application for diet analysis |
title_fullStr |
Carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( Cystophora cristata): Application for diet analysis |
title_full_unstemmed |
Carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( Cystophora cristata): Application for diet analysis |
title_sort |
carbon, nitrogen and sulphur isotopic fractionation in captive juvenile hooded seal ( cystophora cristata): application for diet analysis |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1002/rcm.7955 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.7955 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.7955 |
genre |
Cystophora cristata hooded seal |
genre_facet |
Cystophora cristata hooded seal |
op_source |
Rapid Communications in Mass Spectrometry volume 31, issue 20, page 1720-1728 ISSN 0951-4198 1097-0231 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/rcm.7955 |
container_title |
Rapid Communications in Mass Spectrometry |
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31 |
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20 |
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1720 |
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1728 |
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1802643606092120064 |