Amino acid δ 13 C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons
Abstract We report a novel method for the chromatographic separation and measurement of stable carbon isotope ratios ( δ 13 C) of individual amino acids in hair proteins and bone collagen using the LC‐IsoLink system, which interfaces liquid chromatography (LC) with isotope ratio mass spectrometry (I...
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crwiley:10.1002/rcm.4398 2024-09-30T14:35:59+00:00 Amino acid δ 13 C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons Raghavan, Maanasa McCullagh, James S. O. Lynnerup, Niels Hedges, Robert E. M. 2010 http://dx.doi.org/10.1002/rcm.4398 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.4398 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.4398 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Rapid Communications in Mass Spectrometry volume 24, issue 5, page 541-548 ISSN 0951-4198 1097-0231 journal-article 2010 crwiley https://doi.org/10.1002/rcm.4398 2024-09-17T04:51:18Z Abstract We report a novel method for the chromatographic separation and measurement of stable carbon isotope ratios ( δ 13 C) of individual amino acids in hair proteins and bone collagen using the LC‐IsoLink system, which interfaces liquid chromatography (LC) with isotope ratio mass spectrometry (IRMS). This paper provides baseline separation of 15 and 13 of the 18 amino acids in bone collagen and hair proteins, respectively. We also describe an approach to analysing small hair samples for compound‐specific analysis of segmental hair sections. The LC/IRMS method is applied in a historical context by the δ 13 C analysis of hair proteins and bone collagen recovered from six individuals from Uummannaq in Greenland. The analysis of hair and bone amino acids from the same individual, compared for the first time in this study, is of importance in palaeodietary reconstruction. If hair proteins can be used as a proxy for bone collagen at the amino acid level, this validates compound‐specific isotope studies using hair as a model for palaeodietary reconstruction. Our results suggest that a small offset observed in the bulk δ 13 C values of the hair and bone samples may be attributed to two factors: (i) amino acid compositional differences between hair and bone proteins, and (ii) differential turnover rates of the tissues and the amino acid pools contributing to their synthesis. This application proposes that hair may be a useful complementary or alternative source of compound‐specific paleodietary information. Copyright © 2010 John Wiley & Sons, Ltd. Article in Journal/Newspaper Greenland Uummannaq Wiley Online Library Greenland Rapid Communications in Mass Spectrometry 24 5 541 548 |
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Wiley Online Library |
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English |
description |
Abstract We report a novel method for the chromatographic separation and measurement of stable carbon isotope ratios ( δ 13 C) of individual amino acids in hair proteins and bone collagen using the LC‐IsoLink system, which interfaces liquid chromatography (LC) with isotope ratio mass spectrometry (IRMS). This paper provides baseline separation of 15 and 13 of the 18 amino acids in bone collagen and hair proteins, respectively. We also describe an approach to analysing small hair samples for compound‐specific analysis of segmental hair sections. The LC/IRMS method is applied in a historical context by the δ 13 C analysis of hair proteins and bone collagen recovered from six individuals from Uummannaq in Greenland. The analysis of hair and bone amino acids from the same individual, compared for the first time in this study, is of importance in palaeodietary reconstruction. If hair proteins can be used as a proxy for bone collagen at the amino acid level, this validates compound‐specific isotope studies using hair as a model for palaeodietary reconstruction. Our results suggest that a small offset observed in the bulk δ 13 C values of the hair and bone samples may be attributed to two factors: (i) amino acid compositional differences between hair and bone proteins, and (ii) differential turnover rates of the tissues and the amino acid pools contributing to their synthesis. This application proposes that hair may be a useful complementary or alternative source of compound‐specific paleodietary information. Copyright © 2010 John Wiley & Sons, Ltd. |
format |
Article in Journal/Newspaper |
author |
Raghavan, Maanasa McCullagh, James S. O. Lynnerup, Niels Hedges, Robert E. M. |
spellingShingle |
Raghavan, Maanasa McCullagh, James S. O. Lynnerup, Niels Hedges, Robert E. M. Amino acid δ 13 C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons |
author_facet |
Raghavan, Maanasa McCullagh, James S. O. Lynnerup, Niels Hedges, Robert E. M. |
author_sort |
Raghavan, Maanasa |
title |
Amino acid δ 13 C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons |
title_short |
Amino acid δ 13 C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons |
title_full |
Amino acid δ 13 C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons |
title_fullStr |
Amino acid δ 13 C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons |
title_full_unstemmed |
Amino acid δ 13 C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons |
title_sort |
amino acid δ 13 c analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra‐individual comparisons |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1002/rcm.4398 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.4398 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.4398 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Uummannaq |
genre_facet |
Greenland Uummannaq |
op_source |
Rapid Communications in Mass Spectrometry volume 24, issue 5, page 541-548 ISSN 0951-4198 1097-0231 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/rcm.4398 |
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Rapid Communications in Mass Spectrometry |
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24 |
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5 |
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541 |
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548 |
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1811639172629266432 |