Analysis of organochlorines in harbor seal ( Phoca vitulina) tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique
Abstract A gas chromatography/ion trap mass spectrometry (GC/ITMS) method was developed for the determination of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polychlorinated naphthalenes (PCNs) in harbor seal ( Phoca vitulina ) tissues. Tissue samples were homogenized, lyop...
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crwiley:10.1002/rcm.1990 2024-06-02T08:07:46+00:00 Analysis of organochlorines in harbor seal ( Phoca vitulina) tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique Wang, Dongli Atkinson, Shannon Hoover‐Miller, Anne Li, Qing X. 2005 http://dx.doi.org/10.1002/rcm.1990 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.1990 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.1990 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Rapid Communications in Mass Spectrometry volume 19, issue 13, page 1815-1821 ISSN 0951-4198 1097-0231 journal-article 2005 crwiley https://doi.org/10.1002/rcm.1990 2024-05-03T10:33:17Z Abstract A gas chromatography/ion trap mass spectrometry (GC/ITMS) method was developed for the determination of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polychlorinated naphthalenes (PCNs) in harbor seal ( Phoca vitulina ) tissues. Tissue samples were homogenized, lyophilized and fortified with 13 C‐PCBs 28, 123, 169 and 170, and then extracted with an accelerated solvent extractor with a mixture of hexane and methylene chloride (1:1, v/v). After lipid removal using a 40% H 2 SO 4 ‐modified silica gel column, all organochlorines were collected in one fraction and further fractionated with an activated carbon/silica gel (1:20) column into a first fraction containing OCPs, non‐coplanar PCBs and 13 C‐PCBs 28, 123 and 170, and a second containing PCNs, coplanar PCBs and 13 C‐PCB 169. Prior to GC/MS/MS analysis, 13 C‐PCB 169 was added into the first fraction as an injection standard and 13 C‐PCB 170 into the second fraction to calibrate the recoveries of the fortified internal standards. This method can effectively eliminate matrix interferences, and has high selectivity and sensitivity. Recoveries averaged 45–86% for OCPs with relative standard deviations (RSDs) of 2–14%, 52–137% for PCBs with RSDs of 3–29% and 36–152% for PCNs with RSDs of 7–29% from lard and chicken heart samples, which were used as alternative matrices to harbor seal samples in recovery studies. The limits of detection for OCPs, PCBs and PCNs were 0.7–1.9, 1.5–8.9 and 0.5–10 pg/g dry weight, respectively. This method can be used to analyze OCPs, PCBs and PCNs in harbor seal blubber, liver and kidney samples. Copyright © 2005 John Wiley & Sons, Ltd. Article in Journal/Newspaper harbor seal Phoca vitulina Alaska Wiley Online Library Rapid Communications in Mass Spectrometry 19 13 1815 1821 |
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Abstract A gas chromatography/ion trap mass spectrometry (GC/ITMS) method was developed for the determination of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polychlorinated naphthalenes (PCNs) in harbor seal ( Phoca vitulina ) tissues. Tissue samples were homogenized, lyophilized and fortified with 13 C‐PCBs 28, 123, 169 and 170, and then extracted with an accelerated solvent extractor with a mixture of hexane and methylene chloride (1:1, v/v). After lipid removal using a 40% H 2 SO 4 ‐modified silica gel column, all organochlorines were collected in one fraction and further fractionated with an activated carbon/silica gel (1:20) column into a first fraction containing OCPs, non‐coplanar PCBs and 13 C‐PCBs 28, 123 and 170, and a second containing PCNs, coplanar PCBs and 13 C‐PCB 169. Prior to GC/MS/MS analysis, 13 C‐PCB 169 was added into the first fraction as an injection standard and 13 C‐PCB 170 into the second fraction to calibrate the recoveries of the fortified internal standards. This method can effectively eliminate matrix interferences, and has high selectivity and sensitivity. Recoveries averaged 45–86% for OCPs with relative standard deviations (RSDs) of 2–14%, 52–137% for PCBs with RSDs of 3–29% and 36–152% for PCNs with RSDs of 7–29% from lard and chicken heart samples, which were used as alternative matrices to harbor seal samples in recovery studies. The limits of detection for OCPs, PCBs and PCNs were 0.7–1.9, 1.5–8.9 and 0.5–10 pg/g dry weight, respectively. This method can be used to analyze OCPs, PCBs and PCNs in harbor seal blubber, liver and kidney samples. Copyright © 2005 John Wiley & Sons, Ltd. |
format |
Article in Journal/Newspaper |
author |
Wang, Dongli Atkinson, Shannon Hoover‐Miller, Anne Li, Qing X. |
spellingShingle |
Wang, Dongli Atkinson, Shannon Hoover‐Miller, Anne Li, Qing X. Analysis of organochlorines in harbor seal ( Phoca vitulina) tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique |
author_facet |
Wang, Dongli Atkinson, Shannon Hoover‐Miller, Anne Li, Qing X. |
author_sort |
Wang, Dongli |
title |
Analysis of organochlorines in harbor seal ( Phoca vitulina) tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique |
title_short |
Analysis of organochlorines in harbor seal ( Phoca vitulina) tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique |
title_full |
Analysis of organochlorines in harbor seal ( Phoca vitulina) tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique |
title_fullStr |
Analysis of organochlorines in harbor seal ( Phoca vitulina) tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique |
title_full_unstemmed |
Analysis of organochlorines in harbor seal ( Phoca vitulina) tissue samples from Alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique |
title_sort |
analysis of organochlorines in harbor seal ( phoca vitulina) tissue samples from alaska using gas chromatography/ion trap mass spectrometry by an isotopic dilution technique |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1002/rcm.1990 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.1990 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.1990 |
genre |
harbor seal Phoca vitulina Alaska |
genre_facet |
harbor seal Phoca vitulina Alaska |
op_source |
Rapid Communications in Mass Spectrometry volume 19, issue 13, page 1815-1821 ISSN 0951-4198 1097-0231 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/rcm.1990 |
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Rapid Communications in Mass Spectrometry |
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19 |
container_issue |
13 |
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1815 |
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1821 |
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1800752886415622144 |