High‐throughput simultaneous determination of plasma water deuterium and 18‐oxygen enrichment using a high‐temperature conversion elemental analyzer with isotope ratio mass spectrometry

• Published in: Rapid Communications in Mass Spectrometry
• Main Authors: Richelle, M., Darimont, C., Piguet‐Welsch, C., Fay, L. B.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2004
• Subjects: Greenland; antartic*; GISP; Ice Sheet
• Online Access: http://dx.doi.org/10.1002/rcm.1406; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.1406; https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.1406

Abstract This paper presents a high‐throughput method for the simultaneous determination of deuterium and oxygen‐18 ( 18 O) enrichment of water samples isolated from blood. This analytical method enables rapid and simple determination of these enrichments of microgram quantities of water. Water is converted into hydrogen and carbon monoxide gases by the use of a high‐temperature conversion elemental analyzer (TC‐EA), that are then transferred on‐line into the isotope ratio mass spectrometer. Accuracy determined with the standard light Antartic precipitation (SLAP) and Greenland ice sheet precipitation (GISP) is reliable for deuterium and 18 O enrichments. The range of linearity is from 0 up to 0.09 atom percent excess (APE, i.e. −78 up to 5725 delta per mil (dpm)) for deuterium enrichment and from 0 up to 0.17 APE (−11 up to 890 dpm) for 18 O enrichment. Memory effects do exist but can be avoided by analyzing the biological samples in quintuplet. This method allows the determination of 1440 samples per week, i.e. 288 biological samples per week. Copyright © 2004 John Wiley & Sons, Ltd.