Phe‐46(CD4) orients the distal histidine for hydrogen bonding to bound ligands in sperm whale myoglobin

• Published in: Proteins: Structure, Function, and Genetics
• Main Authors: Lai, Henry H., Li, Tiansheng, Lyons, Daniel S., Phillips, George N., Olson, John S., Gibson, Quentin H.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1995
• Subjects: Sperm whale
• Online Access: https://doi.org/10.1002/prot.340220404; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fprot.340220404; https://onlinelibrary.wiley.com/doi/pdf/10.1002/prot.340220404

Abstract The role of Phe‐46(CD4) in modulating the functional properties of sperm whale myoglobin was investigated by replacing this residue with Leu, Ile, Val, Ala, Trp, Tyr, and Glu. This highly conserved amino acid almost makes direct contact with the distal histidine and has been postulated to affect ligand binding. The overall association rate constants for CO, O 2 , and NO binding were little affected by decreasing the size of residue 46 step‐wise from Phe to Leu to Val to Ala. In contrast, the rates of CO, O 2 , and NO dissociation increased 4‐, 10‐, and 25‐fold, respectively, for the same series of mutants, causing large decreases in the affinity of myoglobin for all three diatomic gases. The rates of autooxidation at 37°C, pH 7.0 increased dramatically from ∼0.1–0.3 h −1 for wild‐type, Tyr‐46, and Trp‐46 myoglobins to 1.5, 5.2, 4.9, and 5.0 h −1 for the Leu‐46, Ile‐46, Val‐46, and Ala‐46 mutants, respectively. Rates of NO and O 2 geminate recombination were measured using 35 ps and 9 ns laser excitation pulses. Decreasing the size of residue 46 causes significant decreases in the extent of both picosecond and nanosecond rebinding processes. High resolution structures of Leu‐46 and Val‐46 metmyoglobins, Val‐46 CO‐myoglobin, and Val‐46 deoxymyoglobin were determined by X‐ray crystallography. When Phe‐46 is replaced by Val, the loss of internal packing volume is compensated by (1) contraction of the CD corner toward the core of the protein, (2) movement of the E‐helix toward the mutation site, (3) greater exposure of the distal pocket to intruding solvent molecules, and (4) large disorder in the position of the side chain of the distal histidine (His‐64). In wild‐type myoglobin, the van der Waals contact between C ζ of Phe‐46 and C β of His‐64 appears to restrict rotation of the imidazole side chain. Insertion of Val at position 46 relieves this steric restriction, allowing the imidazole side chain to rotate about the C α –C β bond toward the surface of the globin and about the C β –C γ bond toward the ...