Crystal structure of myoglobin form a synthetic gene
Abstract Crystal have been grown of myoglobin produced in Escherichia coli from a synthetic gene, and the structure has been solved to 1.9 Å resolution. The space group of the crystals is P6, which is different from previously solved myoglobin crystal forms. The synthetic myoglobin is essentially id...
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crwiley:10.1002/prot.340070407 2024-06-02T08:14:52+00:00 Crystal structure of myoglobin form a synthetic gene Phillips, George N. Arduini, Robert M. Springer, Barry A. Sligar, Stephen G. 1990 http://dx.doi.org/10.1002/prot.340070407 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fprot.340070407 https://onlinelibrary.wiley.com/doi/pdf/10.1002/prot.340070407 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Proteins: Structure, Function, and Bioinformatics volume 7, issue 4, page 358-365 ISSN 0887-3585 1097-0134 journal-article 1990 crwiley https://doi.org/10.1002/prot.340070407 2024-05-03T10:35:07Z Abstract Crystal have been grown of myoglobin produced in Escherichia coli from a synthetic gene, and the structure has been solved to 1.9 Å resolution. The space group of the crystals is P6, which is different from previously solved myoglobin crystal forms. The synthetic myoglobin is essentially identical to myoglobin isolated from sperm whale tissue, except for the retention of the initiator methionine at the N‐terminus and the substitution of asparagine for aspartic acid at position 122. Superposition of the coordinates of native and synthetic sperm whale myoglobins reveals only minor changes in the positions of main chain atoms and roeientation of some surface side chains. Crystals of variant of the “synthetic” myoglobin have also been grown for structural analysis of the role of key amino acid residues in ligand and specificity. Article in Journal/Newspaper Sperm whale Wiley Online Library Proteins: Structure, Function, and Bioinformatics 7 4 358 365 |
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Wiley Online Library |
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language |
English |
description |
Abstract Crystal have been grown of myoglobin produced in Escherichia coli from a synthetic gene, and the structure has been solved to 1.9 Å resolution. The space group of the crystals is P6, which is different from previously solved myoglobin crystal forms. The synthetic myoglobin is essentially identical to myoglobin isolated from sperm whale tissue, except for the retention of the initiator methionine at the N‐terminus and the substitution of asparagine for aspartic acid at position 122. Superposition of the coordinates of native and synthetic sperm whale myoglobins reveals only minor changes in the positions of main chain atoms and roeientation of some surface side chains. Crystals of variant of the “synthetic” myoglobin have also been grown for structural analysis of the role of key amino acid residues in ligand and specificity. |
format |
Article in Journal/Newspaper |
author |
Phillips, George N. Arduini, Robert M. Springer, Barry A. Sligar, Stephen G. |
spellingShingle |
Phillips, George N. Arduini, Robert M. Springer, Barry A. Sligar, Stephen G. Crystal structure of myoglobin form a synthetic gene |
author_facet |
Phillips, George N. Arduini, Robert M. Springer, Barry A. Sligar, Stephen G. |
author_sort |
Phillips, George N. |
title |
Crystal structure of myoglobin form a synthetic gene |
title_short |
Crystal structure of myoglobin form a synthetic gene |
title_full |
Crystal structure of myoglobin form a synthetic gene |
title_fullStr |
Crystal structure of myoglobin form a synthetic gene |
title_full_unstemmed |
Crystal structure of myoglobin form a synthetic gene |
title_sort |
crystal structure of myoglobin form a synthetic gene |
publisher |
Wiley |
publishDate |
1990 |
url |
http://dx.doi.org/10.1002/prot.340070407 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fprot.340070407 https://onlinelibrary.wiley.com/doi/pdf/10.1002/prot.340070407 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Proteins: Structure, Function, and Bioinformatics volume 7, issue 4, page 358-365 ISSN 0887-3585 1097-0134 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/prot.340070407 |
container_title |
Proteins: Structure, Function, and Bioinformatics |
container_volume |
7 |
container_issue |
4 |
container_start_page |
358 |
op_container_end_page |
365 |
_version_ |
1800738861347766272 |