Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa
Abstract A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P2 1 2 1 2 1 . A complete data set to 2.1 Å resolution has been m...
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crwiley:10.1002/pro.5560061121 2024-06-23T07:46:41+00:00 Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa Villeret, Vincent Beeumen, Jozef Van Chessa, Jean‐Pierre Gerday, Charles 1997 http://dx.doi.org/10.1002/pro.5560061121 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpro.5560061121 https://onlinelibrary.wiley.com/doi/pdf/10.1002/pro.5560061121 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Protein Science volume 6, issue 11, page 2462-2464 ISSN 0961-8368 1469-896X journal-article 1997 crwiley https://doi.org/10.1002/pro.5560061121 2024-06-04T06:48:19Z Abstract A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P2 1 2 1 2 1 . A complete data set to 2.1 Å resolution has been measured. The structure has been determined by the molecular replacement method using the coordinates of the mesophilic alkaline protease as a model. The molecular replacement solution displays a correlation coefficient of 0.39 and an R ‐factor of 0.48. Subsequent inspection of the electron density map in the active site region has confirmed the correctness of the solution. Model building and structure refinement will be initiated when the protease sequence becomes fully available. This is the second report, following one on an a‐amylase, of the preliminary crystallographic characterization of a psychrophilic enzyme. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic The Antarctic Protein Science 6 11 2462 2464 |
institution |
Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract A cold alkaline protease, isolated from an Antarctic Pseudomonas aeruginosa strain, has been purified and crystallized. Large crystals were obtained in the presence of PEG 6000 at pH 7 and pH 8. They belong to the space group P2 1 2 1 2 1 . A complete data set to 2.1 Å resolution has been measured. The structure has been determined by the molecular replacement method using the coordinates of the mesophilic alkaline protease as a model. The molecular replacement solution displays a correlation coefficient of 0.39 and an R ‐factor of 0.48. Subsequent inspection of the electron density map in the active site region has confirmed the correctness of the solution. Model building and structure refinement will be initiated when the protease sequence becomes fully available. This is the second report, following one on an a‐amylase, of the preliminary crystallographic characterization of a psychrophilic enzyme. |
format |
Article in Journal/Newspaper |
author |
Villeret, Vincent Beeumen, Jozef Van Chessa, Jean‐Pierre Gerday, Charles |
spellingShingle |
Villeret, Vincent Beeumen, Jozef Van Chessa, Jean‐Pierre Gerday, Charles Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa |
author_facet |
Villeret, Vincent Beeumen, Jozef Van Chessa, Jean‐Pierre Gerday, Charles |
author_sort |
Villeret, Vincent |
title |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa |
title_short |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa |
title_full |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa |
title_fullStr |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa |
title_full_unstemmed |
Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile pseudomonas aeruginosa |
title_sort |
preliminary crystal structure determination of the alkaline protease from the antarctic psychrophile pseudomonas aeruginosa |
publisher |
Wiley |
publishDate |
1997 |
url |
http://dx.doi.org/10.1002/pro.5560061121 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpro.5560061121 https://onlinelibrary.wiley.com/doi/pdf/10.1002/pro.5560061121 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Protein Science volume 6, issue 11, page 2462-2464 ISSN 0961-8368 1469-896X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/pro.5560061121 |
container_title |
Protein Science |
container_volume |
6 |
container_issue |
11 |
container_start_page |
2462 |
op_container_end_page |
2464 |
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1802647530699227136 |