Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the antarctic psychrophile Alteromonas haloplanctis A23
Abstract A cold‐active α‐amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 °C. In order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold‐adapted enzyme have been ini...
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crwiley:10.1002/pro.5560051021 2024-09-15T17:46:03+00:00 Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the antarctic psychrophile Alteromonas haloplanctis A23 Aghajari, Nushin Haser, Richard Feller, Georges Gerday, Charles 1996 http://dx.doi.org/10.1002/pro.5560051021 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpro.5560051021 https://onlinelibrary.wiley.com/doi/pdf/10.1002/pro.5560051021 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Protein Science volume 5, issue 10, page 2128-2129 ISSN 0961-8368 1469-896X journal-article 1996 crwiley https://doi.org/10.1002/pro.5560051021 2024-08-01T04:23:14Z Abstract A cold‐active α‐amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 °C. In order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold‐adapted enzyme have been initiated because a three‐dimensional structure of a mesophilic counterpart, pig pancreatic α‐amylase, already exists. α‐Amylase from A. haloplanctis , which shares 53% sequence identity with pig pancreatic α‐amylase, has been crystallized and data to 1.85 Å have been collected. The space group is found to be C222 1 with a = 71.40 Å, b = 138.88 Å, and c = 115.66 Å. Until now, a three‐dimensional structure of a psychrophilic enzyme is lacking. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Protein Science 5 10 2128 2129 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract A cold‐active α‐amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 °C. In order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold‐adapted enzyme have been initiated because a three‐dimensional structure of a mesophilic counterpart, pig pancreatic α‐amylase, already exists. α‐Amylase from A. haloplanctis , which shares 53% sequence identity with pig pancreatic α‐amylase, has been crystallized and data to 1.85 Å have been collected. The space group is found to be C222 1 with a = 71.40 Å, b = 138.88 Å, and c = 115.66 Å. Until now, a three‐dimensional structure of a psychrophilic enzyme is lacking. |
format |
Article in Journal/Newspaper |
author |
Aghajari, Nushin Haser, Richard Feller, Georges Gerday, Charles |
spellingShingle |
Aghajari, Nushin Haser, Richard Feller, Georges Gerday, Charles Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the antarctic psychrophile Alteromonas haloplanctis A23 |
author_facet |
Aghajari, Nushin Haser, Richard Feller, Georges Gerday, Charles |
author_sort |
Aghajari, Nushin |
title |
Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the antarctic psychrophile Alteromonas haloplanctis A23 |
title_short |
Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the antarctic psychrophile Alteromonas haloplanctis A23 |
title_full |
Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the antarctic psychrophile Alteromonas haloplanctis A23 |
title_fullStr |
Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the antarctic psychrophile Alteromonas haloplanctis A23 |
title_full_unstemmed |
Crystallization and preliminary X‐ray diffraction studies of α‐amylase from the antarctic psychrophile Alteromonas haloplanctis A23 |
title_sort |
crystallization and preliminary x‐ray diffraction studies of α‐amylase from the antarctic psychrophile alteromonas haloplanctis a23 |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1002/pro.5560051021 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpro.5560051021 https://onlinelibrary.wiley.com/doi/pdf/10.1002/pro.5560051021 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Protein Science volume 5, issue 10, page 2128-2129 ISSN 0961-8368 1469-896X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/pro.5560051021 |
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Protein Science |
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5 |
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10 |
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2128 |
op_container_end_page |
2129 |
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1810494003082166272 |