Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters
Abstract Hybrid nano‐biomaterials are exploited in the design and performance of chemo‐enzymatic cascades. In this study, lipase is immobilized from Candida antarctica fraction B (CALB) and gold nanoparticles (Au NPs) on magnetic particles coated with silica (MNP@SiO 2 ) to stepwise hydrolyze and re...
Published in: | Particle & Particle Systems Characterization |
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Online Access: | http://dx.doi.org/10.1002/ppsc.202100136 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ppsc.202100136 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ppsc.202100136 |
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crwiley:10.1002/ppsc.202100136 2024-09-15T17:47:12+00:00 Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters de Barros, Heloise Ribeiro Tanaka, Lívia Yukari da Silva, Rafael Trivella Pacheco Santiago‐Arcos, Javier de Torresi, Susana I. Córdoba López‐Gallego, Fernando Fundação de Amparo à Pesquisa do Estado de São Paulo Fundação de Amparo à Pesquisa do Estado de São Paulo Fundação de Amparo à Pesquisa do Estado de São Paulo Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Agencia Estatal de Investigación 2021 http://dx.doi.org/10.1002/ppsc.202100136 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ppsc.202100136 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ppsc.202100136 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Particle & Particle Systems Characterization volume 38, issue 10 ISSN 0934-0866 1521-4117 journal-article 2021 crwiley https://doi.org/10.1002/ppsc.202100136 2024-08-01T04:23:21Z Abstract Hybrid nano‐biomaterials are exploited in the design and performance of chemo‐enzymatic cascades. In this study, lipase is immobilized from Candida antarctica fraction B (CALB) and gold nanoparticles (Au NPs) on magnetic particles coated with silica (MNP@SiO 2 ) to stepwise hydrolyze and reduce p‐nitrophenyl esters in tandem reaction. The assembly of the two catalysts at the interface of the MNP@SiO 2 particles and the temporal control of the reaction turns out to be the most determinant parameters for the cascade kinetics. When both CALB and Au NPs are co‐immobilized at the MNP@SiO 2 particle, the tandem reactions take place significantly faster than when both catalysts are physically segregated by their immobilization on different MNP@SiO 2 particles. Herein, it is demonstrated that the co‐immobilization of biocatalysts and nanocatalysts in solid materials creates hybrid interfaces that accelerated chemo‐enzymatic tandem reactions. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Particle & Particle Systems Characterization 38 10 |
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Open Polar |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract Hybrid nano‐biomaterials are exploited in the design and performance of chemo‐enzymatic cascades. In this study, lipase is immobilized from Candida antarctica fraction B (CALB) and gold nanoparticles (Au NPs) on magnetic particles coated with silica (MNP@SiO 2 ) to stepwise hydrolyze and reduce p‐nitrophenyl esters in tandem reaction. The assembly of the two catalysts at the interface of the MNP@SiO 2 particles and the temporal control of the reaction turns out to be the most determinant parameters for the cascade kinetics. When both CALB and Au NPs are co‐immobilized at the MNP@SiO 2 particle, the tandem reactions take place significantly faster than when both catalysts are physically segregated by their immobilization on different MNP@SiO 2 particles. Herein, it is demonstrated that the co‐immobilization of biocatalysts and nanocatalysts in solid materials creates hybrid interfaces that accelerated chemo‐enzymatic tandem reactions. |
author2 |
Fundação de Amparo à Pesquisa do Estado de São Paulo Fundação de Amparo à Pesquisa do Estado de São Paulo Fundação de Amparo à Pesquisa do Estado de São Paulo Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Agencia Estatal de Investigación |
format |
Article in Journal/Newspaper |
author |
de Barros, Heloise Ribeiro Tanaka, Lívia Yukari da Silva, Rafael Trivella Pacheco Santiago‐Arcos, Javier de Torresi, Susana I. Córdoba López‐Gallego, Fernando |
spellingShingle |
de Barros, Heloise Ribeiro Tanaka, Lívia Yukari da Silva, Rafael Trivella Pacheco Santiago‐Arcos, Javier de Torresi, Susana I. Córdoba López‐Gallego, Fernando Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters |
author_facet |
de Barros, Heloise Ribeiro Tanaka, Lívia Yukari da Silva, Rafael Trivella Pacheco Santiago‐Arcos, Javier de Torresi, Susana I. Córdoba López‐Gallego, Fernando |
author_sort |
de Barros, Heloise Ribeiro |
title |
Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters |
title_short |
Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters |
title_full |
Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters |
title_fullStr |
Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters |
title_full_unstemmed |
Assembly of Nano‐Biocatalyst for the Tandem Hydrolysis and Reduction of p‐Nitrophenol Esters |
title_sort |
assembly of nano‐biocatalyst for the tandem hydrolysis and reduction of p‐nitrophenol esters |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1002/ppsc.202100136 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ppsc.202100136 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ppsc.202100136 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Particle & Particle Systems Characterization volume 38, issue 10 ISSN 0934-0866 1521-4117 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/ppsc.202100136 |
container_title |
Particle & Particle Systems Characterization |
container_volume |
38 |
container_issue |
10 |
_version_ |
1810496041692168192 |