T‐RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica
ABSTRACT The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice‐free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial communi...
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Online Access: | http://dx.doi.org/10.1002/ppp.1749 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fppp.1749 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ppp.1749 |
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crwiley:10.1002/ppp.1749 2024-06-02T07:58:35+00:00 T‐RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica Abramovich, Reut Sorek Pomati, Francesco Jungblut, Anne D. Guglielmin, Mauro Neilan, Brett A. 2012 http://dx.doi.org/10.1002/ppp.1749 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fppp.1749 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ppp.1749 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Permafrost and Periglacial Processes volume 23, issue 3, page 244-248 ISSN 1045-6740 1099-1530 journal-article 2012 crwiley https://doi.org/10.1002/ppp.1749 2024-05-03T11:18:08Z ABSTRACT The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice‐free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial community profiles within ice and permafrost can be established for these environments, and then whether glaciological differences between the two areas would be reflected in the bacterial community composition. In order to gather first evidence for the bacterial communities in these glacial zones, we carried out terminal‐restriction fragment length polymorphism (T‐RFLP) analysis on the 16S rRNA gene using a universal bacterial amplification protocol on two permafrost cores. The DNA yields from ice‐core samples ranged from 0.29 ng μL ‐1 in Amorphous Glacier to 88 ng μL ‐1 in Boulder Clay. Bray‐Curtis cluster analysis suggested Boulder Clay bacterial profiles were similar to each other, but cluster separately from the Amorphous Glacier bacterial profile. Copyright © 2012 John Wiley & Sons, Ltd. Article in Journal/Newspaper Antarc* Antarctica Ice ice core permafrost Permafrost and Periglacial Processes Victoria Land Wiley Online Library Amorphous Glacier ENVELOPE(163.933,163.933,-74.700,-74.700) Bray ENVELOPE(-114.067,-114.067,-74.833,-74.833) Victoria Land Permafrost and Periglacial Processes 23 3 244 248 |
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Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
ABSTRACT The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice‐free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial community profiles within ice and permafrost can be established for these environments, and then whether glaciological differences between the two areas would be reflected in the bacterial community composition. In order to gather first evidence for the bacterial communities in these glacial zones, we carried out terminal‐restriction fragment length polymorphism (T‐RFLP) analysis on the 16S rRNA gene using a universal bacterial amplification protocol on two permafrost cores. The DNA yields from ice‐core samples ranged from 0.29 ng μL ‐1 in Amorphous Glacier to 88 ng μL ‐1 in Boulder Clay. Bray‐Curtis cluster analysis suggested Boulder Clay bacterial profiles were similar to each other, but cluster separately from the Amorphous Glacier bacterial profile. Copyright © 2012 John Wiley & Sons, Ltd. |
format |
Article in Journal/Newspaper |
author |
Abramovich, Reut Sorek Pomati, Francesco Jungblut, Anne D. Guglielmin, Mauro Neilan, Brett A. |
spellingShingle |
Abramovich, Reut Sorek Pomati, Francesco Jungblut, Anne D. Guglielmin, Mauro Neilan, Brett A. T‐RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
author_facet |
Abramovich, Reut Sorek Pomati, Francesco Jungblut, Anne D. Guglielmin, Mauro Neilan, Brett A. |
author_sort |
Abramovich, Reut Sorek |
title |
T‐RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_short |
T‐RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_full |
T‐RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_fullStr |
T‐RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_full_unstemmed |
T‐RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_sort |
t‐rflp fingerprinting analysis of bacterial communities in debris cones, northern victoria land, antarctica |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1002/ppp.1749 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fppp.1749 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ppp.1749 |
long_lat |
ENVELOPE(163.933,163.933,-74.700,-74.700) ENVELOPE(-114.067,-114.067,-74.833,-74.833) |
geographic |
Amorphous Glacier Bray Victoria Land |
geographic_facet |
Amorphous Glacier Bray Victoria Land |
genre |
Antarc* Antarctica Ice ice core permafrost Permafrost and Periglacial Processes Victoria Land |
genre_facet |
Antarc* Antarctica Ice ice core permafrost Permafrost and Periglacial Processes Victoria Land |
op_source |
Permafrost and Periglacial Processes volume 23, issue 3, page 244-248 ISSN 1045-6740 1099-1530 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/ppp.1749 |
container_title |
Permafrost and Periglacial Processes |
container_volume |
23 |
container_issue |
3 |
container_start_page |
244 |
op_container_end_page |
248 |
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1800741990550208512 |