Rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons
Abstract Laser‐scanning confocal microscopy (LSCM), electron microcopy (EM), and cellular electrophysiology were used in combination to study the structural basis of an inhibitory synapse between two identified neurons of the same network. To achieve this, we examined the chemical inhibitory synapse...
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crwiley:10.1002/neu.10023 2024-06-02T08:07:51+00:00 Rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons Cabirol‐Pol, Marie‐Jeanne Combes, Denis Fénelon, Valérie S Simmers, John Meyrand, Pierre Conseil Régional d'Aquitaine (France) Human Frontier Science Program Ministère de l'Enseignement Supérieur et de la Recherche 2002 http://dx.doi.org/10.1002/neu.10023 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fneu.10023 https://onlinelibrary.wiley.com/doi/pdf/10.1002/neu.10023 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Neurobiology volume 50, issue 2, page 150-163 ISSN 0022-3034 1097-4695 journal-article 2002 crwiley https://doi.org/10.1002/neu.10023 2024-05-03T11:53:03Z Abstract Laser‐scanning confocal microscopy (LSCM), electron microcopy (EM), and cellular electrophysiology were used in combination to study the structural basis of an inhibitory synapse between two identified neurons of the same network. To achieve this, we examined the chemical inhibitory synapse between identified neurons belonging to the lobster ( Homarus gammarus ) pyloric network: the pyloric dilator (PD) and the lateral pyloric (LP) neurons. In order to visualize simultaneously these two neurons, we used intrasomatic injection of Lucifer Yellow (LY) in one and rhodamine/horseradish peroxydase (HRP) in the other. Under LSCM, we found only two zones of close apposition in a restricted part of the neuritic tree of the two network neurons. Then, within these two zones, the synaptic release sites were searched using EM. To this end, photoconversion of LY with immunogold and development of HRP with DAB were performed on the previously observed preparations. Structural evidence was found for only one release site per zone. To confirm this result, and because the zones of contact were always segregated in a restricted part of the dendrites, we used laser photoablation to selectively delete, either pre‐ or postsynaptically, the branches on which the release sites were located. In both cases, such restrictive ablation completely abolished the functional interaction between these neurons. Our results therefore demonstrate that an inhibitory synapse that is essential for the operation of a neural network relies on only very few sites of contact localized in a highly restricted part of each neuron's dendritic arbor. © 2002 Wiley Periodicals, Inc. J Neurobiol 50: 150–163, 2002; DOI 10.1002/neu.10023 Article in Journal/Newspaper Homarus gammarus Wiley Online Library Journal of Neurobiology 50 2 150 163 |
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English |
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Abstract Laser‐scanning confocal microscopy (LSCM), electron microcopy (EM), and cellular electrophysiology were used in combination to study the structural basis of an inhibitory synapse between two identified neurons of the same network. To achieve this, we examined the chemical inhibitory synapse between identified neurons belonging to the lobster ( Homarus gammarus ) pyloric network: the pyloric dilator (PD) and the lateral pyloric (LP) neurons. In order to visualize simultaneously these two neurons, we used intrasomatic injection of Lucifer Yellow (LY) in one and rhodamine/horseradish peroxydase (HRP) in the other. Under LSCM, we found only two zones of close apposition in a restricted part of the neuritic tree of the two network neurons. Then, within these two zones, the synaptic release sites were searched using EM. To this end, photoconversion of LY with immunogold and development of HRP with DAB were performed on the previously observed preparations. Structural evidence was found for only one release site per zone. To confirm this result, and because the zones of contact were always segregated in a restricted part of the dendrites, we used laser photoablation to selectively delete, either pre‐ or postsynaptically, the branches on which the release sites were located. In both cases, such restrictive ablation completely abolished the functional interaction between these neurons. Our results therefore demonstrate that an inhibitory synapse that is essential for the operation of a neural network relies on only very few sites of contact localized in a highly restricted part of each neuron's dendritic arbor. © 2002 Wiley Periodicals, Inc. J Neurobiol 50: 150–163, 2002; DOI 10.1002/neu.10023 |
author2 |
Conseil Régional d'Aquitaine (France) Human Frontier Science Program Ministère de l'Enseignement Supérieur et de la Recherche |
format |
Article in Journal/Newspaper |
author |
Cabirol‐Pol, Marie‐Jeanne Combes, Denis Fénelon, Valérie S Simmers, John Meyrand, Pierre |
spellingShingle |
Cabirol‐Pol, Marie‐Jeanne Combes, Denis Fénelon, Valérie S Simmers, John Meyrand, Pierre Rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons |
author_facet |
Cabirol‐Pol, Marie‐Jeanne Combes, Denis Fénelon, Valérie S Simmers, John Meyrand, Pierre |
author_sort |
Cabirol‐Pol, Marie‐Jeanne |
title |
Rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons |
title_short |
Rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons |
title_full |
Rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons |
title_fullStr |
Rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons |
title_full_unstemmed |
Rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons |
title_sort |
rare and spatially segregated release sites mediate a synaptic interaction between two identified network neurons |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1002/neu.10023 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fneu.10023 https://onlinelibrary.wiley.com/doi/pdf/10.1002/neu.10023 |
genre |
Homarus gammarus |
genre_facet |
Homarus gammarus |
op_source |
Journal of Neurobiology volume 50, issue 2, page 150-163 ISSN 0022-3034 1097-4695 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/neu.10023 |
container_title |
Journal of Neurobiology |
container_volume |
50 |
container_issue |
2 |
container_start_page |
150 |
op_container_end_page |
163 |
_version_ |
1800752983317676032 |