CO 2 induced pH i changes in the brain of polar fish: a TauCEST application
Chemical exchange saturation transfer (CEST) from taurine to water (TauCEST) can be used for in vivo mapping of taurine concentrations as well as for measurements of relative changes in intracellular pH (pH i ) at temperatures below 37°C. Therefore, TauCEST offers the opportunity to investigate acid...
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crwiley:10.1002/nbm.3955 2024-06-02T08:04:30+00:00 CO 2 induced pH i changes in the brain of polar fish: a TauCEST application Wermter, Felizitas C. Maus, Bastian Pörtner, Hans‐O. Dreher, Wolfgang Bock, Christian Deutsche Forschungsgemeinschaft 2018 http://dx.doi.org/10.1002/nbm.3955 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fnbm.3955 http://onlinelibrary.wiley.com/wol1/doi/10.1002/nbm.3955/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor NMR in Biomedicine volume 31, issue 8 ISSN 0952-3480 1099-1492 journal-article 2018 crwiley https://doi.org/10.1002/nbm.3955 2024-05-03T10:53:46Z Chemical exchange saturation transfer (CEST) from taurine to water (TauCEST) can be used for in vivo mapping of taurine concentrations as well as for measurements of relative changes in intracellular pH (pH i ) at temperatures below 37°C. Therefore, TauCEST offers the opportunity to investigate acid–base regulation and neurological disturbances of ectothermic animals living at low temperatures, and in particular to study the impact of ocean acidification (OA) on neurophysiological changes of fish. Here, we report the first in vivo application of TauCEST imaging. Thus, the study aimed to investigate the TauCEST effect in a broad range of temperatures (1–37°C) and pH (5.5–8.0), motivated by the high taurine concentration measured in the brains of polar fish. The in vitro data show that the TauCEST effect is especially detectable in the low temperature range and strictly monotonic for the relevant pH range (6.8–7.5). To investigate the specificity of TauCEST imaging for the brain of polar cod ( Boreogadus saida ) at 1.5°C simulations were carried out, indicating a taurine contribution of about 65% to the in vivo expected CEST effect, if experimental parameters are optimized. B. saida was acutely exposed to three different CO 2 concentrations in the sea water (control normocapnia; comparatively moderate hypercapnia OA m = 3300 μatm; high hypercapnia OA h = 4900 μatm). TauCEST imaging of the brain showed a significant increase in the TauCEST effect under the different CO 2 concentrations of about 1.5–3% in comparison with control measurements, indicative of changes in pH i or metabolite concentration. Consecutive recordings of 1 H MR spectra gave no support for a concentration induced change of the in vivo observed TauCEST effect. Thus, the in vivo application of TauCEST offers the possibility of mapping relative changes in pH i in the brain of polar cod during exposure to CO 2 . Article in Journal/Newspaper Boreogadus saida Ocean acidification polar cod Wiley Online Library NMR in Biomedicine 31 8 |
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Wiley Online Library |
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English |
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Chemical exchange saturation transfer (CEST) from taurine to water (TauCEST) can be used for in vivo mapping of taurine concentrations as well as for measurements of relative changes in intracellular pH (pH i ) at temperatures below 37°C. Therefore, TauCEST offers the opportunity to investigate acid–base regulation and neurological disturbances of ectothermic animals living at low temperatures, and in particular to study the impact of ocean acidification (OA) on neurophysiological changes of fish. Here, we report the first in vivo application of TauCEST imaging. Thus, the study aimed to investigate the TauCEST effect in a broad range of temperatures (1–37°C) and pH (5.5–8.0), motivated by the high taurine concentration measured in the brains of polar fish. The in vitro data show that the TauCEST effect is especially detectable in the low temperature range and strictly monotonic for the relevant pH range (6.8–7.5). To investigate the specificity of TauCEST imaging for the brain of polar cod ( Boreogadus saida ) at 1.5°C simulations were carried out, indicating a taurine contribution of about 65% to the in vivo expected CEST effect, if experimental parameters are optimized. B. saida was acutely exposed to three different CO 2 concentrations in the sea water (control normocapnia; comparatively moderate hypercapnia OA m = 3300 μatm; high hypercapnia OA h = 4900 μatm). TauCEST imaging of the brain showed a significant increase in the TauCEST effect under the different CO 2 concentrations of about 1.5–3% in comparison with control measurements, indicative of changes in pH i or metabolite concentration. Consecutive recordings of 1 H MR spectra gave no support for a concentration induced change of the in vivo observed TauCEST effect. Thus, the in vivo application of TauCEST offers the possibility of mapping relative changes in pH i in the brain of polar cod during exposure to CO 2 . |
author2 |
Deutsche Forschungsgemeinschaft |
format |
Article in Journal/Newspaper |
author |
Wermter, Felizitas C. Maus, Bastian Pörtner, Hans‐O. Dreher, Wolfgang Bock, Christian |
spellingShingle |
Wermter, Felizitas C. Maus, Bastian Pörtner, Hans‐O. Dreher, Wolfgang Bock, Christian CO 2 induced pH i changes in the brain of polar fish: a TauCEST application |
author_facet |
Wermter, Felizitas C. Maus, Bastian Pörtner, Hans‐O. Dreher, Wolfgang Bock, Christian |
author_sort |
Wermter, Felizitas C. |
title |
CO 2 induced pH i changes in the brain of polar fish: a TauCEST application |
title_short |
CO 2 induced pH i changes in the brain of polar fish: a TauCEST application |
title_full |
CO 2 induced pH i changes in the brain of polar fish: a TauCEST application |
title_fullStr |
CO 2 induced pH i changes in the brain of polar fish: a TauCEST application |
title_full_unstemmed |
CO 2 induced pH i changes in the brain of polar fish: a TauCEST application |
title_sort |
co 2 induced ph i changes in the brain of polar fish: a taucest application |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/nbm.3955 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fnbm.3955 http://onlinelibrary.wiley.com/wol1/doi/10.1002/nbm.3955/fullpdf |
genre |
Boreogadus saida Ocean acidification polar cod |
genre_facet |
Boreogadus saida Ocean acidification polar cod |
op_source |
NMR in Biomedicine volume 31, issue 8 ISSN 0952-3480 1099-1492 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/nbm.3955 |
container_title |
NMR in Biomedicine |
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31 |
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8 |
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1800749130202480640 |