Effects of Reaction Operation Policies on Properties of Core–Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts
Abstract Core–shell polymer supports with different morphological features and compositions are prepared through combined suspension and emulsion polymerizations. It is shown that proper manipulation of the divinylbenzene (DVB) feed content allows for maximization of specific areas, porosities, and...
Published in: | Macromolecular Reaction Engineering |
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crwiley:10.1002/mren.201800055 2024-09-15T17:46:13+00:00 Effects of Reaction Operation Policies on Properties of Core–Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts Pinto, Martina Costa Cerqueira de Souza e Castro, Nathany Lisbôa Cipolatti, Eliane Pereira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Freire, Denise Maria Guimarães Pinto, José Carlos Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 2018 http://dx.doi.org/10.1002/mren.201800055 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmren.201800055 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mren.201800055 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Macromolecular Reaction Engineering volume 13, issue 1 ISSN 1862-832X 1862-8338 journal-article 2018 crwiley https://doi.org/10.1002/mren.201800055 2024-07-23T04:13:27Z Abstract Core–shell polymer supports with different morphological features and compositions are prepared through combined suspension and emulsion polymerizations. It is shown that proper manipulation of the divinylbenzene (DVB) feed content allows for maximization of specific areas, porosities, and mechanical resistances. Additionally, it is shown that feeding of previously prepared miniemulsions leads to core–shell particles with smaller specific areas, due to less efficient coating of the cores. Particularly, the combined manipulation of polymerization times and DVB feed compositions allows for production of particles with pronounced specific area (50 m 2 g −1 ) and porosity (0.30 cm 3 g −1 ). Produced core–shell polymer particles are employed as supports for the immobilization of lipase B from Candida antarctica , and the obtained enzymatic activities for both hydrolysis ( A hyd ) and esterification ( A est ) reactions are very high ( A hyd = 34.7 ± 3.8 U/g; A est = 3564.6 ± 581 U/g), even when compared to activities obtained using the reference commercial biocatalyst Novozym 435 ( A hyd = 7.6 ± 1.8 U/g, A est = 2384.7 ± 307.2 U/g). Finally, biocatalysts prepared with the core–shell supports present higher enzymatic activities than biocatalysts prepared with supports of higher specific area obtained through conventional emulsion polymerizations, indicating that the porous structure of the shell can be beneficial for the immobilization and activity of the enzymes. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Macromolecular Reaction Engineering 13 1 |
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English |
description |
Abstract Core–shell polymer supports with different morphological features and compositions are prepared through combined suspension and emulsion polymerizations. It is shown that proper manipulation of the divinylbenzene (DVB) feed content allows for maximization of specific areas, porosities, and mechanical resistances. Additionally, it is shown that feeding of previously prepared miniemulsions leads to core–shell particles with smaller specific areas, due to less efficient coating of the cores. Particularly, the combined manipulation of polymerization times and DVB feed compositions allows for production of particles with pronounced specific area (50 m 2 g −1 ) and porosity (0.30 cm 3 g −1 ). Produced core–shell polymer particles are employed as supports for the immobilization of lipase B from Candida antarctica , and the obtained enzymatic activities for both hydrolysis ( A hyd ) and esterification ( A est ) reactions are very high ( A hyd = 34.7 ± 3.8 U/g; A est = 3564.6 ± 581 U/g), even when compared to activities obtained using the reference commercial biocatalyst Novozym 435 ( A hyd = 7.6 ± 1.8 U/g, A est = 2384.7 ± 307.2 U/g). Finally, biocatalysts prepared with the core–shell supports present higher enzymatic activities than biocatalysts prepared with supports of higher specific area obtained through conventional emulsion polymerizations, indicating that the porous structure of the shell can be beneficial for the immobilization and activity of the enzymes. |
author2 |
Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior |
format |
Article in Journal/Newspaper |
author |
Pinto, Martina Costa Cerqueira de Souza e Castro, Nathany Lisbôa Cipolatti, Eliane Pereira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Freire, Denise Maria Guimarães Pinto, José Carlos |
spellingShingle |
Pinto, Martina Costa Cerqueira de Souza e Castro, Nathany Lisbôa Cipolatti, Eliane Pereira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Freire, Denise Maria Guimarães Pinto, José Carlos Effects of Reaction Operation Policies on Properties of Core–Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts |
author_facet |
Pinto, Martina Costa Cerqueira de Souza e Castro, Nathany Lisbôa Cipolatti, Eliane Pereira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Freire, Denise Maria Guimarães Pinto, José Carlos |
author_sort |
Pinto, Martina Costa Cerqueira |
title |
Effects of Reaction Operation Policies on Properties of Core–Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts |
title_short |
Effects of Reaction Operation Policies on Properties of Core–Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts |
title_full |
Effects of Reaction Operation Policies on Properties of Core–Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts |
title_fullStr |
Effects of Reaction Operation Policies on Properties of Core–Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts |
title_full_unstemmed |
Effects of Reaction Operation Policies on Properties of Core–Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts |
title_sort |
effects of reaction operation policies on properties of core–shell polymer supports used for preparation of highly active biocatalysts |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/mren.201800055 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmren.201800055 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mren.201800055 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Macromolecular Reaction Engineering volume 13, issue 1 ISSN 1862-832X 1862-8338 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/mren.201800055 |
container_title |
Macromolecular Reaction Engineering |
container_volume |
13 |
container_issue |
1 |
_version_ |
1810494205052583936 |