Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon ( Salmo salar L.) males
Abstract The germ cells are essential for sexual reproduction by giving rise to the gametes, but the importance of germ cells for gonadal somatic functions varies among vertebrates. The RNA‐binding dead end (Dnd) protein is necessary for the specification and migration of primordial germ cells to th...
Published in: | Molecular Reproduction and Development |
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Online Access: | http://dx.doi.org/10.1002/mrd.23635 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.23635 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/mrd.23635 |
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crwiley:10.1002/mrd.23635 2024-06-23T07:51:14+00:00 Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon ( Salmo salar L.) males Tveiten, Helge Karlsen, Kristian Thesslund, Tina Johansson, Gunhild S. Thiyagarajan, Dhivya B. Andersen, Øivind 2022 http://dx.doi.org/10.1002/mrd.23635 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.23635 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/mrd.23635 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Molecular Reproduction and Development volume 89, issue 10, page 471-484 ISSN 1040-452X 1098-2795 journal-article 2022 crwiley https://doi.org/10.1002/mrd.23635 2024-05-31T08:13:32Z Abstract The germ cells are essential for sexual reproduction by giving rise to the gametes, but the importance of germ cells for gonadal somatic functions varies among vertebrates. The RNA‐binding dead end (Dnd) protein is necessary for the specification and migration of primordial germ cells to the future reproductive organs. Here, we ablated the gametes in Atlantic salmon males and females by microinjecting dnd antisense gapmer oligonucleotides at the zygotic stage. Precocious maturation was induced in above 50% of both germ cell‐depleted and intact fertile males, but not in females, by exposure to an off‐season photoperiod regime. Sterile and fertile males showed similar body growth, but maturing fish tended to be heavier than their immature counterparts. Pituitary fshβ messenger RNA levels strongly increased in maturing sterile and fertile males concomitant with the upregulated expression of Sertoli and Leydig cell markers. Plasma concentrations of 11‐ketotestosterone and testosterone in maturing sterile males were significantly higher than the basal levels in immature fish, but lower than those in maturing fertile males. The study demonstrates that germ cells are not a prerequisite for the activation of the brain–pituitary–gonad axis and sex steroidogenesis in Atlantic salmon males, but may be important for the maintenance of gonadal somatic functions. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Molecular Reproduction and Development |
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English |
description |
Abstract The germ cells are essential for sexual reproduction by giving rise to the gametes, but the importance of germ cells for gonadal somatic functions varies among vertebrates. The RNA‐binding dead end (Dnd) protein is necessary for the specification and migration of primordial germ cells to the future reproductive organs. Here, we ablated the gametes in Atlantic salmon males and females by microinjecting dnd antisense gapmer oligonucleotides at the zygotic stage. Precocious maturation was induced in above 50% of both germ cell‐depleted and intact fertile males, but not in females, by exposure to an off‐season photoperiod regime. Sterile and fertile males showed similar body growth, but maturing fish tended to be heavier than their immature counterparts. Pituitary fshβ messenger RNA levels strongly increased in maturing sterile and fertile males concomitant with the upregulated expression of Sertoli and Leydig cell markers. Plasma concentrations of 11‐ketotestosterone and testosterone in maturing sterile males were significantly higher than the basal levels in immature fish, but lower than those in maturing fertile males. The study demonstrates that germ cells are not a prerequisite for the activation of the brain–pituitary–gonad axis and sex steroidogenesis in Atlantic salmon males, but may be important for the maintenance of gonadal somatic functions. |
format |
Article in Journal/Newspaper |
author |
Tveiten, Helge Karlsen, Kristian Thesslund, Tina Johansson, Gunhild S. Thiyagarajan, Dhivya B. Andersen, Øivind |
spellingShingle |
Tveiten, Helge Karlsen, Kristian Thesslund, Tina Johansson, Gunhild S. Thiyagarajan, Dhivya B. Andersen, Øivind Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon ( Salmo salar L.) males |
author_facet |
Tveiten, Helge Karlsen, Kristian Thesslund, Tina Johansson, Gunhild S. Thiyagarajan, Dhivya B. Andersen, Øivind |
author_sort |
Tveiten, Helge |
title |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon ( Salmo salar L.) males |
title_short |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon ( Salmo salar L.) males |
title_full |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon ( Salmo salar L.) males |
title_fullStr |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon ( Salmo salar L.) males |
title_full_unstemmed |
Impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious Atlantic salmon ( Salmo salar L.) males |
title_sort |
impact of germ cell ablation on the activation of the brain–pituitary–gonadal axis in precocious atlantic salmon ( salmo salar l.) males |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1002/mrd.23635 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.23635 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/mrd.23635 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Molecular Reproduction and Development volume 89, issue 10, page 471-484 ISSN 1040-452X 1098-2795 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1002/mrd.23635 |
container_title |
Molecular Reproduction and Development |
_version_ |
1802642264857509888 |